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Hydrogen peroxide in the Burkitt's lymphoma cell line Raji provides protection against arsenic trioxide-induced apoptosis via the phosphoinositide-3 kinase signalling pathway.
Br J Haematol 2004; 125(4):512-20BJ

Abstract

Many anticarcinogenic drugs kill tumour cells by inducing apoptosis. We examined the effects of hydrogen peroxide (H(2)O(2)) on arsenic trioxide (As(2)O(3))-induced cell killing. Low concentrations of H(2)O(2) (200 micromol/l) inhibited the ability of As(2)O(3) to induce apoptosis in the Burkitt's lymphoma cell line Raji. H(2)O(2) altered the form of cell death from apoptosis to pyknosis/necrosis and also lowered the degree of cell killing by As(2)O(3). H(2)O(2) was capable of preventing caspase-3 activation induced by As(2)O(3) in Raji cells. Incubation of cells with a phosphoinositide-3 kinase (PI-3K) inhibitor, wortmannin (100 nmol/l), blocked the effects of H(2)O(2) on As(2)O(3)-induced caspase-3 activation. In addition, the PI-3K inhibitor partially blocked the effects of H(2)O(2) on up-regulation of Bcl-2 and Bcl-X(L) protein expression, down-regulation of Bax protein expression, and phosphorylation of Bcl-2 and IkappaBalpha. This investigation demonstrated for the first time that low concentrations of H(2)O(2) provide protection against the in vivo of As(2)O(3)-induced apoptosis. PI-3K plays a crucial role in enhancing cell survival during H(2)O(2), inhibiting As(2)O(3)-induced apoptosis in the Burkitt's lymphoma cells. As(2)O(3)-induced cancer cell apoptosis may be enhanced by certain antioxidants in the treatment protocol.

Authors+Show Affiliations

Department of Cell Biology, The First Military Medical University, Guangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15142122

Citation

Lu, Di, et al. "Hydrogen Peroxide in the Burkitt's Lymphoma Cell Line Raji Provides Protection Against Arsenic Trioxide-induced Apoptosis Via the Phosphoinositide-3 Kinase Signalling Pathway." British Journal of Haematology, vol. 125, no. 4, 2004, pp. 512-20.
Lu D, Bai XC, Gui L, et al. Hydrogen peroxide in the Burkitt's lymphoma cell line Raji provides protection against arsenic trioxide-induced apoptosis via the phosphoinositide-3 kinase signalling pathway. Br J Haematol. 2004;125(4):512-20.
Lu, D., Bai, X. C., Gui, L., Su, Y. C., Deng, F., Liu, B., ... Luo, S. Q. (2004). Hydrogen peroxide in the Burkitt's lymphoma cell line Raji provides protection against arsenic trioxide-induced apoptosis via the phosphoinositide-3 kinase signalling pathway. British Journal of Haematology, 125(4), pp. 512-20.
Lu D, et al. Hydrogen Peroxide in the Burkitt's Lymphoma Cell Line Raji Provides Protection Against Arsenic Trioxide-induced Apoptosis Via the Phosphoinositide-3 Kinase Signalling Pathway. Br J Haematol. 2004;125(4):512-20. PubMed PMID: 15142122.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrogen peroxide in the Burkitt's lymphoma cell line Raji provides protection against arsenic trioxide-induced apoptosis via the phosphoinositide-3 kinase signalling pathway. AU - Lu,Di, AU - Bai,Xiao-Chun, AU - Gui,Li, AU - Su,Yong-Chun, AU - Deng,Fan, AU - Liu,Bin, AU - Li,Xiu-Mei, AU - Zeng,Wei-Sen, AU - Cheng,Bao-Luan, AU - Luo,Shen-Qiu, PY - 2004/5/15/pubmed PY - 2004/8/31/medline PY - 2004/5/15/entrez SP - 512 EP - 20 JF - British journal of haematology JO - Br. J. Haematol. VL - 125 IS - 4 N2 - Many anticarcinogenic drugs kill tumour cells by inducing apoptosis. We examined the effects of hydrogen peroxide (H(2)O(2)) on arsenic trioxide (As(2)O(3))-induced cell killing. Low concentrations of H(2)O(2) (200 micromol/l) inhibited the ability of As(2)O(3) to induce apoptosis in the Burkitt's lymphoma cell line Raji. H(2)O(2) altered the form of cell death from apoptosis to pyknosis/necrosis and also lowered the degree of cell killing by As(2)O(3). H(2)O(2) was capable of preventing caspase-3 activation induced by As(2)O(3) in Raji cells. Incubation of cells with a phosphoinositide-3 kinase (PI-3K) inhibitor, wortmannin (100 nmol/l), blocked the effects of H(2)O(2) on As(2)O(3)-induced caspase-3 activation. In addition, the PI-3K inhibitor partially blocked the effects of H(2)O(2) on up-regulation of Bcl-2 and Bcl-X(L) protein expression, down-regulation of Bax protein expression, and phosphorylation of Bcl-2 and IkappaBalpha. This investigation demonstrated for the first time that low concentrations of H(2)O(2) provide protection against the in vivo of As(2)O(3)-induced apoptosis. PI-3K plays a crucial role in enhancing cell survival during H(2)O(2), inhibiting As(2)O(3)-induced apoptosis in the Burkitt's lymphoma cells. As(2)O(3)-induced cancer cell apoptosis may be enhanced by certain antioxidants in the treatment protocol. SN - 0007-1048 UR - https://www.unboundmedicine.com/medline/citation/15142122/Hydrogen_peroxide_in_the_Burkitt's_lymphoma_cell_line_Raji_provides_protection_against_arsenic_trioxide_induced_apoptosis_via_the_phosphoinositide_3_kinase_signalling_pathway_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-1048&date=2004&volume=125&issue=4&spage=512 DB - PRIME DP - Unbound Medicine ER -