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Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen.
Arthritis Res Ther. 2004; 6(3):R213-9.AR

Abstract

Induction of oral tolerance has long been considered a promising approach to the treatment of chronic autoimmune diseases, including rheumatoid arthritis (RA). Oral administration of type II collagen (CII) has been proven to improve signs and symptoms in RA patients without troublesome toxicity. To investigate the mechanism of immune suppression mediated by orally administered antigen, we examined changes in serum IgG subtypes and T-cell proliferative responses to CII, and generation of IL-10-producing CD4+CD25+ T-cell subsets in an animal model of collagen-induced arthritis (CIA). We found that joint inflammation in CIA mice peaked at 5 weeks after primary immunization with CII, which was significantly less in mice tolerized by repeated oral feeding of CII before CIA induction. Mice that had been fed with CII also exhibited increased serum IgG1 and decreased serum IgG2a as compared with nontolerized CIA animals. The T-cell proliferative response to CII was suppressed in lymph nodes of tolerized mice also. Production of IL-10 and of transforming growth factor-beta from mononuclear lymphocytes was increased in the tolerized animals, and CD4+ T cells isolated from tolerized mice did not respond with induction of IFN-gamma when stimulated in vitro with CII. We also observed greater induction of IL-10-producing CD4+CD25+ subsets among CII-stimulated splenic T cells from tolerized mice. These data suggest that when these IL-10-producing CD4+CD25+ T cells encounter CII antigen in affected joints they become activated to exert an anti-inflammatory effect.

Authors+Show Affiliations

Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15142267

Citation

Min, So-Youn, et al. "Induction of IL-10-producing CD4+CD25+ T Cells in Animal Model of Collagen-induced Arthritis By Oral Administration of Type II Collagen." Arthritis Research & Therapy, vol. 6, no. 3, 2004, pp. R213-9.
Min SY, Hwang SY, Park KS, et al. Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen. Arthritis Res Ther. 2004;6(3):R213-9.
Min, S. Y., Hwang, S. Y., Park, K. S., Lee, J. S., Lee, K. E., Kim, K. W., Jung, Y. O., Koh, H. J., Do, J. H., Kim, H., & Kim, H. Y. (2004). Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen. Arthritis Research & Therapy, 6(3), R213-9.
Min SY, et al. Induction of IL-10-producing CD4+CD25+ T Cells in Animal Model of Collagen-induced Arthritis By Oral Administration of Type II Collagen. Arthritis Res Ther. 2004;6(3):R213-9. PubMed PMID: 15142267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen. AU - Min,So-Youn, AU - Hwang,Sue-Yun, AU - Park,Kyung-Su, AU - Lee,Jae-Sun, AU - Lee,Kang-Eun, AU - Kim,Kyung-Wun, AU - Jung,Young-Ok, AU - Koh,Hyunk-Jae, AU - Do,Ju-Ho, AU - Kim,Haerim, AU - Kim,Ho-Youn, Y1 - 2004/03/11/ PY - 2003/10/22/received PY - 2004/02/10/revised PY - 2004/02/26/accepted PY - 2004/5/15/pubmed PY - 2005/10/14/medline PY - 2004/5/15/entrez SP - R213 EP - 9 JF - Arthritis research & therapy JO - Arthritis Res. Ther. VL - 6 IS - 3 N2 - Induction of oral tolerance has long been considered a promising approach to the treatment of chronic autoimmune diseases, including rheumatoid arthritis (RA). Oral administration of type II collagen (CII) has been proven to improve signs and symptoms in RA patients without troublesome toxicity. To investigate the mechanism of immune suppression mediated by orally administered antigen, we examined changes in serum IgG subtypes and T-cell proliferative responses to CII, and generation of IL-10-producing CD4+CD25+ T-cell subsets in an animal model of collagen-induced arthritis (CIA). We found that joint inflammation in CIA mice peaked at 5 weeks after primary immunization with CII, which was significantly less in mice tolerized by repeated oral feeding of CII before CIA induction. Mice that had been fed with CII also exhibited increased serum IgG1 and decreased serum IgG2a as compared with nontolerized CIA animals. The T-cell proliferative response to CII was suppressed in lymph nodes of tolerized mice also. Production of IL-10 and of transforming growth factor-beta from mononuclear lymphocytes was increased in the tolerized animals, and CD4+ T cells isolated from tolerized mice did not respond with induction of IFN-gamma when stimulated in vitro with CII. We also observed greater induction of IL-10-producing CD4+CD25+ subsets among CII-stimulated splenic T cells from tolerized mice. These data suggest that when these IL-10-producing CD4+CD25+ T cells encounter CII antigen in affected joints they become activated to exert an anti-inflammatory effect. SN - 1478-6362 UR - https://www.unboundmedicine.com/medline/citation/15142267/Induction_of_IL_10_producing_CD4+CD25+_T_cells_in_animal_model_of_collagen_induced_arthritis_by_oral_administration_of_type_II_collagen_ L2 - https://arthritis-research.biomedcentral.com/articles/10.1186/ar1169 DB - PRIME DP - Unbound Medicine ER -