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Relationship between uptake capacity and differential toxicity of the herbicide atrazine in selected microalgal species.
Aquat Toxicol. 2004 Jun 10; 68(2):121-8.AT

Abstract

Microalgal species vary in their sensitivity to the triazine herbicide, atrazine. This study examined both atrazine uptake and cellular characteristics of microalgae to determine if either can be used to predict algal sensitivity. Standard toxicity tests were performed on five microalgal species, each representing a different algal division or habitat. Test species listed in order of increasing sensitivity were: Isochrysis galbana, Dunaliella tertiolecta, Phaeodactylum tricornutum, Pseudokirchneriella subcapitata, and Synechococcus sp. Each species was exposed to 14C-atrazine at its growth rate EC50 concentration (44-91 microg/L). At five time-points over 96 h, samples were filtered to collect algae and washed with unlabeled atrazine to displace labeled atrazine loosely absorbed to the cell surface. Radioactivity present on filters and in the growth medium was measured by liquid scintillation counting. Relationships between algal species-sensitivity to atrazine and compound uptake, cell dry weight, cell volume, and cell surface area were determined by linear regression analysis. Cell size measurements (based on dry weight, biovolume, and surface area) were significantly correlated with atrazine uptake (R2 > 0.45, P-value < 0.05). There was a significant correlation between atrazine uptake and species-sensitivity to atrazine (R2 = 0.5413 , P-value = 0.0012). These results indicate that smaller cells with greater surface area to volume ratios will incorporate more atrazine, and in general, will be more sensitive to atrazine exposure. However, I. galbana, with small cell size and relatively high atrazine uptake was the least sensitive species tested. This species and others may have mechanisms to compensate for atrazine stress that make predicting responses of microalgal communities difficult.

Authors+Show Affiliations

Weiner JA
Medical University of South Carolina, Marine Biomedicine and Environmental Sciences, 221 Fort Johnson Rd., Charleston, SC 29412, USA.
DeLorenzo ME
No affiliation info available
Fulton MH
No affiliation info available

MeSH

AtrazineBiomassCarbon RadioisotopesEukaryotaHerbicidesLinear ModelsScintillation CountingTime FactorsToxicity TestsWater Pollutants, Chemical

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15145222

Citation

Weiner, Jeannette A., et al. "Relationship Between Uptake Capacity and Differential Toxicity of the Herbicide Atrazine in Selected Microalgal Species." Aquatic Toxicology (Amsterdam, Netherlands), vol. 68, no. 2, 2004, pp. 121-8.
Weiner JA, DeLorenzo ME, Fulton MH. Relationship between uptake capacity and differential toxicity of the herbicide atrazine in selected microalgal species. Aquat Toxicol. 2004;68(2):121-8.
Weiner, J. A., DeLorenzo, M. E., & Fulton, M. H. (2004). Relationship between uptake capacity and differential toxicity of the herbicide atrazine in selected microalgal species. Aquatic Toxicology (Amsterdam, Netherlands), 68(2), 121-8.
Weiner JA, DeLorenzo ME, Fulton MH. Relationship Between Uptake Capacity and Differential Toxicity of the Herbicide Atrazine in Selected Microalgal Species. Aquat Toxicol. 2004 Jun 10;68(2):121-8. PubMed PMID: 15145222.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship between uptake capacity and differential toxicity of the herbicide atrazine in selected microalgal species. AU - Weiner,Jeannette A, AU - DeLorenzo,Marie E, AU - Fulton,Michael H, PY - 2003/03/07/received PY - 2004/02/20/revised PY - 2004/03/03/accepted PY - 2004/5/18/pubmed PY - 2004/8/26/medline PY - 2004/5/18/entrez SP - 121 EP - 8 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 68 IS - 2 N2 - Microalgal species vary in their sensitivity to the triazine herbicide, atrazine. This study examined both atrazine uptake and cellular characteristics of microalgae to determine if either can be used to predict algal sensitivity. Standard toxicity tests were performed on five microalgal species, each representing a different algal division or habitat. Test species listed in order of increasing sensitivity were: Isochrysis galbana, Dunaliella tertiolecta, Phaeodactylum tricornutum, Pseudokirchneriella subcapitata, and Synechococcus sp. Each species was exposed to 14C-atrazine at its growth rate EC50 concentration (44-91 microg/L). At five time-points over 96 h, samples were filtered to collect algae and washed with unlabeled atrazine to displace labeled atrazine loosely absorbed to the cell surface. Radioactivity present on filters and in the growth medium was measured by liquid scintillation counting. Relationships between algal species-sensitivity to atrazine and compound uptake, cell dry weight, cell volume, and cell surface area were determined by linear regression analysis. Cell size measurements (based on dry weight, biovolume, and surface area) were significantly correlated with atrazine uptake (R2 > 0.45, P-value < 0.05). There was a significant correlation between atrazine uptake and species-sensitivity to atrazine (R2 = 0.5413 , P-value = 0.0012). These results indicate that smaller cells with greater surface area to volume ratios will incorporate more atrazine, and in general, will be more sensitive to atrazine exposure. However, I. galbana, with small cell size and relatively high atrazine uptake was the least sensitive species tested. This species and others may have mechanisms to compensate for atrazine stress that make predicting responses of microalgal communities difficult. SN - 0166-445X UR - https://www.unboundmedicine.com/medline/citation/15145222/Relationship_between_uptake_capacity_and_differential_toxicity_of_the_herbicide_atrazine_in_selected_microalgal_species_ DB - PRIME DP - Unbound Medicine ER -