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A novel and effective approach of developing aggressive experimental autoimmune gastritis in neonatal thymectomized BALB/c mouse by polyinosinic:polycytidylic acid.
Clin Exp Immunol 2004; 136(3):423-31CE

Abstract

Neonatal thymectomy induces autoimmune gastritis (AIG) in 40-70% of BALB/c mice. We presumed that induction of autoimmunity by polyinosinic:polycytidylic acid (poly I:C) might allow development of a more aggressive model of AIG. A group of BALB/c mice were thymectomized on day 3 after birth. Neonatal thymectomized mice were either injected with poly I:C or phosphate-buffered saline (PBS). Non thymectomized neonatal BALB/c mice were injected with only poly I:C. All neonatal thymectomized mice injected with poly I:C developed 3 cardinal features of AIG: (1) moderate to severe degree gastritis (2) presence of autoantibody to H(+)/K(+) ATPase and (3) loss of parietal cells. However, only 70% of the PBS-treated neonatal thymectomized BALB/c mice developed some, but not, all features of AIG. A mild degree of AIG was seen in 12 of 31 nonthymectomized BALB/c mice administered with only poly I:C. Administration of poly I:C in neonatal thymectomized BALB/c mice in the first and second week appeared to be the most effective for induction of aggressive AIG. The levels of interleukin (IL)-6, IL-12p70, interferon-gamma and tumour necrosis factor-alpha were significantly higher in poly I:C-injected thymectomized mice compared to PBS-injected neonatal thymectomized mice (P < 0.05). The frequencies of CD4(+)CD25(+) regulatory T cells in the spleen were significantly decreased in neonatal thymectomized mice administered with poly I:C compared to PBS-treated neonatal thymectomized mice (P < 0.01). Taken together, these results suggest that induction of inflammatory cytokines and reduction of regulatory T cells by poly I:C might contribute to the development of an aggressive model of AIG in neonatal thymectomized BALB/c mice.

Authors+Show Affiliations

Third Department of Internal Medicine, Ehime University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15147343

Citation

Kobayashi, Y, et al. "A Novel and Effective Approach of Developing Aggressive Experimental Autoimmune Gastritis in Neonatal Thymectomized BALB/c Mouse By Polyinosinic:polycytidylic Acid." Clinical and Experimental Immunology, vol. 136, no. 3, 2004, pp. 423-31.
Kobayashi Y, Murakami H, Akbar SM, et al. A novel and effective approach of developing aggressive experimental autoimmune gastritis in neonatal thymectomized BALB/c mouse by polyinosinic:polycytidylic acid. Clin Exp Immunol. 2004;136(3):423-31.
Kobayashi, Y., Murakami, H., Akbar, S. M., Matsui, H., & Onji, M. (2004). A novel and effective approach of developing aggressive experimental autoimmune gastritis in neonatal thymectomized BALB/c mouse by polyinosinic:polycytidylic acid. Clinical and Experimental Immunology, 136(3), pp. 423-31.
Kobayashi Y, et al. A Novel and Effective Approach of Developing Aggressive Experimental Autoimmune Gastritis in Neonatal Thymectomized BALB/c Mouse By Polyinosinic:polycytidylic Acid. Clin Exp Immunol. 2004;136(3):423-31. PubMed PMID: 15147343.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel and effective approach of developing aggressive experimental autoimmune gastritis in neonatal thymectomized BALB/c mouse by polyinosinic:polycytidylic acid. AU - Kobayashi,Y, AU - Murakami,H, AU - Akbar,S M F, AU - Matsui,H, AU - Onji,M, PY - 2004/5/19/pubmed PY - 2004/6/26/medline PY - 2004/5/19/entrez SP - 423 EP - 31 JF - Clinical and experimental immunology JO - Clin. Exp. Immunol. VL - 136 IS - 3 N2 - Neonatal thymectomy induces autoimmune gastritis (AIG) in 40-70% of BALB/c mice. We presumed that induction of autoimmunity by polyinosinic:polycytidylic acid (poly I:C) might allow development of a more aggressive model of AIG. A group of BALB/c mice were thymectomized on day 3 after birth. Neonatal thymectomized mice were either injected with poly I:C or phosphate-buffered saline (PBS). Non thymectomized neonatal BALB/c mice were injected with only poly I:C. All neonatal thymectomized mice injected with poly I:C developed 3 cardinal features of AIG: (1) moderate to severe degree gastritis (2) presence of autoantibody to H(+)/K(+) ATPase and (3) loss of parietal cells. However, only 70% of the PBS-treated neonatal thymectomized BALB/c mice developed some, but not, all features of AIG. A mild degree of AIG was seen in 12 of 31 nonthymectomized BALB/c mice administered with only poly I:C. Administration of poly I:C in neonatal thymectomized BALB/c mice in the first and second week appeared to be the most effective for induction of aggressive AIG. The levels of interleukin (IL)-6, IL-12p70, interferon-gamma and tumour necrosis factor-alpha were significantly higher in poly I:C-injected thymectomized mice compared to PBS-injected neonatal thymectomized mice (P < 0.05). The frequencies of CD4(+)CD25(+) regulatory T cells in the spleen were significantly decreased in neonatal thymectomized mice administered with poly I:C compared to PBS-treated neonatal thymectomized mice (P < 0.01). Taken together, these results suggest that induction of inflammatory cytokines and reduction of regulatory T cells by poly I:C might contribute to the development of an aggressive model of AIG in neonatal thymectomized BALB/c mice. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/15147343/A_novel_and_effective_approach_of_developing_aggressive_experimental_autoimmune_gastritis_in_neonatal_thymectomized_BALB/c_mouse_by_polyinosinic:polycytidylic_acid_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=0009-9104&amp;date=2004&amp;volume=136&amp;issue=3&amp;spage=423 DB - PRIME DP - Unbound Medicine ER -