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Effects of mercury and selenite on delta-aminolevulinate dehydratase activity and on selected oxidative stress parameters in rats.
Environ Res. 2004 Jun; 95(2):166-73.ER

Abstract

The present study evaluates the effects of Na(2)SeO(3) and HgCl(2) on kidney and liver of adult rats. In vivo, HgCl(2) (17 micromol/kg, sc) reduced ascorbic acid levels in liver (approximately 15%), whereas in kidney it reduced ALA-D activity (approximately 60%) and ascorbic acid levels (approximately 35%) and increased TBARS content (approximately 50%). Na(2)SeO(3) (17 micromol/kg, sc) exposure increased the content of nonprotein thiol groups in liver (35-60%) and kidney (approximately 50-160%), partially prevented mercury-induced ALA-D inhibition in kidney, and completely prevented a mercury-induced increase of TBARS content and decrease of ascorbic acid levels in kidney. In vitro, HgCl(2) and Na(2)SeO(3) inhibited renal and hepatic ALA-D, while HgCl(2) increased TBARS in renal and hepatic tissue preparations. Na(2)SeO(3) increased the rate of glutathione oxidation in vitro. Results indicated that Na(2)SeO(3) protected against HgCl(2) effects in vivo (prevention of mercury interaction with thiol groups and of mercury-induced oxidative damage). In vitro, Na(2)SeO(3) did not prevent mercury effects, but potentiated ALA-D inhibition by mercury, probably due to its ability to oxidize thiol groups.

Authors+Show Affiliations

Master Science Course in Biochemical Toxicology, Department of Chemistry, Center of Nature and Exacts Sciences, Federal University of Santa Maria, 97105-900 Santa Maria, RS, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15147922

Citation

Perottoni, Juliano, et al. "Effects of Mercury and Selenite On Delta-aminolevulinate Dehydratase Activity and On Selected Oxidative Stress Parameters in Rats." Environmental Research, vol. 95, no. 2, 2004, pp. 166-73.
Perottoni J, Lobato LP, Silveira A, et al. Effects of mercury and selenite on delta-aminolevulinate dehydratase activity and on selected oxidative stress parameters in rats. Environ Res. 2004;95(2):166-73.
Perottoni, J., Lobato, L. P., Silveira, A., Rocha, J. B., & Emanuelli, T. (2004). Effects of mercury and selenite on delta-aminolevulinate dehydratase activity and on selected oxidative stress parameters in rats. Environmental Research, 95(2), 166-73.
Perottoni J, et al. Effects of Mercury and Selenite On Delta-aminolevulinate Dehydratase Activity and On Selected Oxidative Stress Parameters in Rats. Environ Res. 2004;95(2):166-73. PubMed PMID: 15147922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of mercury and selenite on delta-aminolevulinate dehydratase activity and on selected oxidative stress parameters in rats. AU - Perottoni,Juliano, AU - Lobato,Luciana P, AU - Silveira,Aline, AU - Rocha,João Batista Teixeira, AU - Emanuelli,Tatiana, PY - 2003/04/10/received PY - 2003/08/14/revised PY - 2003/08/29/accepted PY - 2004/5/19/pubmed PY - 2004/7/29/medline PY - 2004/5/19/entrez SP - 166 EP - 73 JF - Environmental research JO - Environ Res VL - 95 IS - 2 N2 - The present study evaluates the effects of Na(2)SeO(3) and HgCl(2) on kidney and liver of adult rats. In vivo, HgCl(2) (17 micromol/kg, sc) reduced ascorbic acid levels in liver (approximately 15%), whereas in kidney it reduced ALA-D activity (approximately 60%) and ascorbic acid levels (approximately 35%) and increased TBARS content (approximately 50%). Na(2)SeO(3) (17 micromol/kg, sc) exposure increased the content of nonprotein thiol groups in liver (35-60%) and kidney (approximately 50-160%), partially prevented mercury-induced ALA-D inhibition in kidney, and completely prevented a mercury-induced increase of TBARS content and decrease of ascorbic acid levels in kidney. In vitro, HgCl(2) and Na(2)SeO(3) inhibited renal and hepatic ALA-D, while HgCl(2) increased TBARS in renal and hepatic tissue preparations. Na(2)SeO(3) increased the rate of glutathione oxidation in vitro. Results indicated that Na(2)SeO(3) protected against HgCl(2) effects in vivo (prevention of mercury interaction with thiol groups and of mercury-induced oxidative damage). In vitro, Na(2)SeO(3) did not prevent mercury effects, but potentiated ALA-D inhibition by mercury, probably due to its ability to oxidize thiol groups. SN - 0013-9351 UR - https://www.unboundmedicine.com/medline/citation/15147922/Effects_of_mercury_and_selenite_on_delta_aminolevulinate_dehydratase_activity_and_on_selected_oxidative_stress_parameters_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0013935103001683 DB - PRIME DP - Unbound Medicine ER -