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Epidermolysis bullosa acquisita sera react with distinct epitopes on the NC1 and NC2 domains of type VII collagen: study using immunoblotting of domain-specific recombinant proteins and postembedding immunoelectron microscopy.
Br J Dermatol. 2004 May; 150(5):843-51.BJ

Abstract

BACKGROUND

The sera of epidermolysis bullosa acquisita (EBA) react with type VII collagen, a major component of anchoring fibrils, in which the major epitopes have been considered to be present in the N-terminal noncollagenous (NC) 1 domain.

OBJECTIVES

To determine whether there are also epitopes in the C-terminal NC2 domain, and to determine their ultrastructural localization.

METHODS

Immunoblotting using recombinant proteins of the NC1 and NC2 domains of type VII collagen, and postembedding immunoelectron microscopy.

RESULTS

Twenty of 28 EBA sera tested reacted with the NC1 domain and eight sera reacted with the NC2 domain. The sera that reacted with the NC1 domain showed immunoreactivity within the lamina densa and the sera that reacted with the NC2 domain showed immunoreactivity in the dermis 300-360 nm below the lamina densa.

CONCLUSIONS

This study clearly identified the presence of epitopes in the NC2 domain, and showed that the epitope in the NC1 domain is present in the lamina densa and that the epitope in the NC2 domain is in the dermis below the lamina densa.

Authors+Show Affiliations

Department of Dermatology, Kurume University School of Medicine, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15149495

Citation

Ishii, N, et al. "Epidermolysis Bullosa Acquisita Sera React With Distinct Epitopes On the NC1 and NC2 Domains of Type VII Collagen: Study Using Immunoblotting of Domain-specific Recombinant Proteins and Postembedding Immunoelectron Microscopy." The British Journal of Dermatology, vol. 150, no. 5, 2004, pp. 843-51.
Ishii N, Yoshida M, Hisamatsu Y, et al. Epidermolysis bullosa acquisita sera react with distinct epitopes on the NC1 and NC2 domains of type VII collagen: study using immunoblotting of domain-specific recombinant proteins and postembedding immunoelectron microscopy. Br J Dermatol. 2004;150(5):843-51.
Ishii, N., Yoshida, M., Hisamatsu, Y., Ishida-Yamamoto, A., Nakane, H., Iizuka, H., Tanaka, T., & Hashimoto, T. (2004). Epidermolysis bullosa acquisita sera react with distinct epitopes on the NC1 and NC2 domains of type VII collagen: study using immunoblotting of domain-specific recombinant proteins and postembedding immunoelectron microscopy. The British Journal of Dermatology, 150(5), 843-51.
Ishii N, et al. Epidermolysis Bullosa Acquisita Sera React With Distinct Epitopes On the NC1 and NC2 Domains of Type VII Collagen: Study Using Immunoblotting of Domain-specific Recombinant Proteins and Postembedding Immunoelectron Microscopy. Br J Dermatol. 2004;150(5):843-51. PubMed PMID: 15149495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epidermolysis bullosa acquisita sera react with distinct epitopes on the NC1 and NC2 domains of type VII collagen: study using immunoblotting of domain-specific recombinant proteins and postembedding immunoelectron microscopy. AU - Ishii,N, AU - Yoshida,M, AU - Hisamatsu,Y, AU - Ishida-Yamamoto,A, AU - Nakane,H, AU - Iizuka,H, AU - Tanaka,T, AU - Hashimoto,T, PY - 2004/5/20/pubmed PY - 2004/7/16/medline PY - 2004/5/20/entrez SP - 843 EP - 51 JF - The British journal of dermatology JO - Br J Dermatol VL - 150 IS - 5 N2 - BACKGROUND: The sera of epidermolysis bullosa acquisita (EBA) react with type VII collagen, a major component of anchoring fibrils, in which the major epitopes have been considered to be present in the N-terminal noncollagenous (NC) 1 domain. OBJECTIVES: To determine whether there are also epitopes in the C-terminal NC2 domain, and to determine their ultrastructural localization. METHODS: Immunoblotting using recombinant proteins of the NC1 and NC2 domains of type VII collagen, and postembedding immunoelectron microscopy. RESULTS: Twenty of 28 EBA sera tested reacted with the NC1 domain and eight sera reacted with the NC2 domain. The sera that reacted with the NC1 domain showed immunoreactivity within the lamina densa and the sera that reacted with the NC2 domain showed immunoreactivity in the dermis 300-360 nm below the lamina densa. CONCLUSIONS: This study clearly identified the presence of epitopes in the NC2 domain, and showed that the epitope in the NC1 domain is present in the lamina densa and that the epitope in the NC2 domain is in the dermis below the lamina densa. SN - 0007-0963 UR - https://www.unboundmedicine.com/medline/citation/15149495/Epidermolysis_bullosa_acquisita_sera_react_with_distinct_epitopes_on_the_NC1_and_NC2_domains_of_type_VII_collagen:_study_using_immunoblotting_of_domain_specific_recombinant_proteins_and_postembedding_immunoelectron_microscopy_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-0963&date=2004&volume=150&issue=5&spage=843 DB - PRIME DP - Unbound Medicine ER -