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PAR-3 is required for epithelial cell polarity in the distal spermatheca of C. elegans.
Development. 2004 Jun; 131(12):2865-74.D

Abstract

PAR-3 is localized asymmetrically in epithelial cells in a variety of animals from Caenorhabditis elegans to mammals. Although C. elegans PAR-3 is known to act in early blastomeres to polarize the embryo, a role for PAR-3 in epithelial cells of C. elegans has not been established. Using RNA interference to deplete PAR-3 in developing larvae, we discovered a requirement for PAR-3 in spermathecal development. Spermathecal precursor cells are born during larval development and differentiate into an epithelium that forms a tube for the storage of sperm. Eggs must enter the spermatheca to complete ovulation. PAR-3-depleted worms exhibit defects in ovulation. Consistent with this phenotype, PAR-3 is transiently expressed and localized asymmetrically in the developing somatic gonad, including the spermathecal precursor cells of L4 larvae. We found that the defect in ovulation can be partially suppressed by a mutation in IPP-5, an inositol polyphosphate 5-phosphatase, indicating that one effect of PAR-3 depletion is disruption of signaling between oocyte and spermatheca. Microscopy revealed that the distribution of AJM-1, an apical junction marker, and apical microfilaments are severely affected in the distal spermatheca of PAR-3-depleted worms. We propose that PAR-3 activity is required for the proper polarization of spermathecal cells and that defective ovulation results from defective distal spermathecal development.

Authors+Show Affiliations

Department of Molecular Biology and Genetics, Cornell University, 107 Biotechnology Building, Ithaca, NY 14853, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15151982

Citation

Aono, Shinya, et al. "PAR-3 Is Required for Epithelial Cell Polarity in the Distal Spermatheca of C. Elegans." Development (Cambridge, England), vol. 131, no. 12, 2004, pp. 2865-74.
Aono S, Legouis R, Hoose WA, et al. PAR-3 is required for epithelial cell polarity in the distal spermatheca of C. elegans. Development. 2004;131(12):2865-74.
Aono, S., Legouis, R., Hoose, W. A., & Kemphues, K. J. (2004). PAR-3 is required for epithelial cell polarity in the distal spermatheca of C. elegans. Development (Cambridge, England), 131(12), 2865-74.
Aono S, et al. PAR-3 Is Required for Epithelial Cell Polarity in the Distal Spermatheca of C. Elegans. Development. 2004;131(12):2865-74. PubMed PMID: 15151982.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PAR-3 is required for epithelial cell polarity in the distal spermatheca of C. elegans. AU - Aono,Shinya, AU - Legouis,Renaud, AU - Hoose,Wendy A, AU - Kemphues,Kenneth J, Y1 - 2004/05/19/ PY - 2004/5/21/pubmed PY - 2004/8/11/medline PY - 2004/5/21/entrez SP - 2865 EP - 74 JF - Development (Cambridge, England) JO - Development VL - 131 IS - 12 N2 - PAR-3 is localized asymmetrically in epithelial cells in a variety of animals from Caenorhabditis elegans to mammals. Although C. elegans PAR-3 is known to act in early blastomeres to polarize the embryo, a role for PAR-3 in epithelial cells of C. elegans has not been established. Using RNA interference to deplete PAR-3 in developing larvae, we discovered a requirement for PAR-3 in spermathecal development. Spermathecal precursor cells are born during larval development and differentiate into an epithelium that forms a tube for the storage of sperm. Eggs must enter the spermatheca to complete ovulation. PAR-3-depleted worms exhibit defects in ovulation. Consistent with this phenotype, PAR-3 is transiently expressed and localized asymmetrically in the developing somatic gonad, including the spermathecal precursor cells of L4 larvae. We found that the defect in ovulation can be partially suppressed by a mutation in IPP-5, an inositol polyphosphate 5-phosphatase, indicating that one effect of PAR-3 depletion is disruption of signaling between oocyte and spermatheca. Microscopy revealed that the distribution of AJM-1, an apical junction marker, and apical microfilaments are severely affected in the distal spermatheca of PAR-3-depleted worms. We propose that PAR-3 activity is required for the proper polarization of spermathecal cells and that defective ovulation results from defective distal spermathecal development. SN - 0950-1991 UR - https://www.unboundmedicine.com/medline/citation/15151982/PAR_3_is_required_for_epithelial_cell_polarity_in_the_distal_spermatheca_of_C__elegans_ L2 - http://dev.biologists.org/cgi/pmidlookup?view=long&pmid=15151982 DB - PRIME DP - Unbound Medicine ER -