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Identification of the pollen determinant of S-RNase-mediated self-incompatibility.
Nature. 2004 May 20; 429(6989):302-5.Nat

Abstract

Many flowering plants have adopted self-incompatibility mechanisms to prevent inbreeding and promote out-crosses. In the Solanaceae, Rosaceae and Scrophulariaceae, two separate genes at the highly polymorphic S-locus control self-incompatibility interactions: the S-RNase gene encodes the pistil determinant and the previously unidentified S-gene encodes the pollen determinant. S-RNases interact with pollen S-allele products to inhibit the growth of self-pollen tubes in the style. Pollen-expressed F-box genes showing allelic sequence polymorphism have recently been identified near to the S-RNase gene in members of the Rosaceae and Scrophulariaceae; but until now have not been directly shown to encode the pollen determinant. Here we report the identification and characterization of PiSLF, an S-locus F-box gene of Petunia inflata (Solanaceae). We show that transformation of S1S1, S1S2 and S2S3 plants with the S2-allele of PiSLF causes breakdown of their pollen function in self-incompatibility. This breakdown of pollen function is consistent with 'competitive interaction', in which pollen carrying two different pollen S-alleles fails to function in self-incompatibility. We conclude that PiSLF encodes the pollen self-incompatibility determinant.

Authors+Show Affiliations

Integrative Biosciences Graduate Degree Program, The Pennsylvania State University, 403 Althouse Laboratory, University Park, Pennsylvania 16802, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15152253

Citation

Sijacic, Paja, et al. "Identification of the Pollen Determinant of S-RNase-mediated Self-incompatibility." Nature, vol. 429, no. 6989, 2004, pp. 302-5.
Sijacic P, Wang X, Skirpan AL, et al. Identification of the pollen determinant of S-RNase-mediated self-incompatibility. Nature. 2004;429(6989):302-5.
Sijacic, P., Wang, X., Skirpan, A. L., Wang, Y., Dowd, P. E., McCubbin, A. G., Huang, S., & Kao, T. H. (2004). Identification of the pollen determinant of S-RNase-mediated self-incompatibility. Nature, 429(6989), 302-5.
Sijacic P, et al. Identification of the Pollen Determinant of S-RNase-mediated Self-incompatibility. Nature. 2004 May 20;429(6989):302-5. PubMed PMID: 15152253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of the pollen determinant of S-RNase-mediated self-incompatibility. AU - Sijacic,Paja, AU - Wang,Xi, AU - Skirpan,Andrea L, AU - Wang,Yan, AU - Dowd,Peter E, AU - McCubbin,Andrew G, AU - Huang,Shihshieh, AU - Kao,Teh-Hui, PY - 2003/12/17/received PY - 2004/03/30/accepted PY - 2004/5/21/pubmed PY - 2004/5/29/medline PY - 2004/5/21/entrez SP - 302 EP - 5 JF - Nature JO - Nature VL - 429 IS - 6989 N2 - Many flowering plants have adopted self-incompatibility mechanisms to prevent inbreeding and promote out-crosses. In the Solanaceae, Rosaceae and Scrophulariaceae, two separate genes at the highly polymorphic S-locus control self-incompatibility interactions: the S-RNase gene encodes the pistil determinant and the previously unidentified S-gene encodes the pollen determinant. S-RNases interact with pollen S-allele products to inhibit the growth of self-pollen tubes in the style. Pollen-expressed F-box genes showing allelic sequence polymorphism have recently been identified near to the S-RNase gene in members of the Rosaceae and Scrophulariaceae; but until now have not been directly shown to encode the pollen determinant. Here we report the identification and characterization of PiSLF, an S-locus F-box gene of Petunia inflata (Solanaceae). We show that transformation of S1S1, S1S2 and S2S3 plants with the S2-allele of PiSLF causes breakdown of their pollen function in self-incompatibility. This breakdown of pollen function is consistent with 'competitive interaction', in which pollen carrying two different pollen S-alleles fails to function in self-incompatibility. We conclude that PiSLF encodes the pollen self-incompatibility determinant. SN - 1476-4687 UR - https://www.unboundmedicine.com/medline/citation/15152253/Identification_of_the_pollen_determinant_of_S_RNase_mediated_self_incompatibility_ L2 - https://doi.org/10.1038/nature02523 DB - PRIME DP - Unbound Medicine ER -