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Glial cell expression of hepatocyte growth factor in vitreoretinal proliferative disease.
Lab Invest. 2004 Aug; 84(8):963-72.LI

Abstract

The hepatocyte growth factor (HGF) has been crucially implicated in the development of proliferative retinal diseases; however, it is unclear whether retinal glial cells express or respond to HGF. Therefore, we examined the expression of HGF and of the receptor for HGF, c-Met, by immunohistochemical costaining with glial fibrillary acidic protein (GFAP) in epiretinal membranes of patients with proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR), respectively. Furthermore, it was determined whether cells of the human retinal glial cell line, MIO-M1, secrete HGF protein, and whether HGF stimulates proliferation and chemotaxis, and secretion of the vascular endothelial growth factor (VEGF). Neuroretinas of patients with PVR express elevated mRNA level for HGF in comparison to control retinas. In epiretinal membranes of patients with PVR or PDR, immunoreactivity for HGF and for c-Met, respectively, partially colocalized with immunoreactivity for GFAP. Fetal bovine serum and basic fibroblast growth factor, but not heparin-binding epidermal or platelet-derived growth factors, evoked HGF secretion by cultured retinal glial cells. HGF displayed only a marginal effect on cell proliferation while it stimulated chemotaxis. HGF promoted the secretion of VEGF, via activation of the phosphatidylinositol-3 kinase. It is concluded that glial cells in epiretinal membranes express both HGF protein and c-Met receptors. The results suggest an autocrine/paracrine role of HGF in glial cell responses during proliferative vitreoretinal disorders as well as in retinal neovascularization, by stimulating of VEGF release.

Authors+Show Affiliations

Department of Ophthalmology, University Eye Clinic, Leipzig, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15156160

Citation

Hollborn, Margrit, et al. "Glial Cell Expression of Hepatocyte Growth Factor in Vitreoretinal Proliferative Disease." Laboratory Investigation; a Journal of Technical Methods and Pathology, vol. 84, no. 8, 2004, pp. 963-72.
Hollborn M, Krausse C, Iandiev I, et al. Glial cell expression of hepatocyte growth factor in vitreoretinal proliferative disease. Lab Invest. 2004;84(8):963-72.
Hollborn, M., Krausse, C., Iandiev, I., Yafai, Y., Tenckhoff, S., Bigl, M., Schnurrbusch, U. E., Limb, G. A., Reichenbach, A., Kohen, L., Wolf, S., Wiedemann, P., & Bringmann, A. (2004). Glial cell expression of hepatocyte growth factor in vitreoretinal proliferative disease. Laboratory Investigation; a Journal of Technical Methods and Pathology, 84(8), 963-72.
Hollborn M, et al. Glial Cell Expression of Hepatocyte Growth Factor in Vitreoretinal Proliferative Disease. Lab Invest. 2004;84(8):963-72. PubMed PMID: 15156160.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glial cell expression of hepatocyte growth factor in vitreoretinal proliferative disease. AU - Hollborn,Margrit, AU - Krausse,Christian, AU - Iandiev,Ianors, AU - Yafai,Yousef, AU - Tenckhoff,Solveig, AU - Bigl,Marina, AU - Schnurrbusch,Ute E K, AU - Limb,G Astrid, AU - Reichenbach,Andreas, AU - Kohen,Leon, AU - Wolf,Sebastian, AU - Wiedemann,Peter, AU - Bringmann,Andreas, PY - 2004/5/25/pubmed PY - 2004/9/3/medline PY - 2004/5/25/entrez SP - 963 EP - 72 JF - Laboratory investigation; a journal of technical methods and pathology JO - Lab. Invest. VL - 84 IS - 8 N2 - The hepatocyte growth factor (HGF) has been crucially implicated in the development of proliferative retinal diseases; however, it is unclear whether retinal glial cells express or respond to HGF. Therefore, we examined the expression of HGF and of the receptor for HGF, c-Met, by immunohistochemical costaining with glial fibrillary acidic protein (GFAP) in epiretinal membranes of patients with proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR), respectively. Furthermore, it was determined whether cells of the human retinal glial cell line, MIO-M1, secrete HGF protein, and whether HGF stimulates proliferation and chemotaxis, and secretion of the vascular endothelial growth factor (VEGF). Neuroretinas of patients with PVR express elevated mRNA level for HGF in comparison to control retinas. In epiretinal membranes of patients with PVR or PDR, immunoreactivity for HGF and for c-Met, respectively, partially colocalized with immunoreactivity for GFAP. Fetal bovine serum and basic fibroblast growth factor, but not heparin-binding epidermal or platelet-derived growth factors, evoked HGF secretion by cultured retinal glial cells. HGF displayed only a marginal effect on cell proliferation while it stimulated chemotaxis. HGF promoted the secretion of VEGF, via activation of the phosphatidylinositol-3 kinase. It is concluded that glial cells in epiretinal membranes express both HGF protein and c-Met receptors. The results suggest an autocrine/paracrine role of HGF in glial cell responses during proliferative vitreoretinal disorders as well as in retinal neovascularization, by stimulating of VEGF release. SN - 0023-6837 UR - https://www.unboundmedicine.com/medline/citation/15156160/Glial_cell_expression_of_hepatocyte_growth_factor_in_vitreoretinal_proliferative_disease_ L2 - http://dx.doi.org/10.1038/labinvest.3700121 DB - PRIME DP - Unbound Medicine ER -