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Postsynaptic localization of gamma-aminobutyric acid transporters and receptors in the outer plexiform layer of the goldfish retina: An ultrastructural study.
J Comp Neurol 2004; 474(1):58-74JC

Abstract

The gamma-aminobutyric acid (GABA)-ergic system in the outer plexiform layer (OPL) of the goldfish retina was studied via light and electron immunohistochemistry. The subcellular distributions of immunoreactivity (-IR) of plasma membrane GABA transporters GAT2 and GAT3, the alpha1 and alpha3 subunits of the ionotropic GABA(A) receptor, and the rho1 subunit of the ionotropic GABA(C) receptor were determined. The localization of the GAT2-IR and GAT3-IR to horizontal cell dendrites at the base of the cone synaptic complex was the main characteristic at the ultrastructural level. Very rarely, GAT2-IR and GAT3-IR were found in horizontal cell dendrites innervating rod spherules. alpha1-IR and alpha3-IR were seen in wide bands in the OPL, whereas rho1-IR appeared as a narrow band in the OPL. Most alpha1-IR was intracellular in rod and cone terminals. Membrane-associated alpha1-IR was observed in cone pedicles but not in rod spherules; postsynaptic elements were also labeled. alpha3-IR was concentrated in the lateral elements of horizontal cell dendrites in cone pedicles. In contrast, rho1-IR was found mainly on the spinules of the horizontal cell dendrites in cone pedicles. In addition, in another type of cone pedicle, rho1-IR was found at the position of OFF-bipolar cell dendrites. alpha3-IR and rho1-IR were rarely found in horizontal cell dendrites innervating rods. We suggest that two GABAergic pathways exist in the outer retina- first, a GABAergic positive loop with GABA receptors mainly on the horizontal cell dendrites and spinules and, second, a GABAergic feedback pathway involving GABA receptors on cone pedicles and GABA transporters on horizontal cells and that this pathway presumably modulates feedback strength from horizontal cells to cones.

Authors+Show Affiliations

Department of Retinal Signal Processing, Netherlands Ophthalmic Research Institute-KNAW, 1105 BA Amsterdam, The Netherlands. j.klooster@ioi.knaw.nlNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15156579

Citation

Klooster, Jan, et al. "Postsynaptic Localization of Gamma-aminobutyric Acid Transporters and Receptors in the Outer Plexiform Layer of the Goldfish Retina: an Ultrastructural Study." The Journal of Comparative Neurology, vol. 474, no. 1, 2004, pp. 58-74.
Klooster J, Nunes Cardozo B, Yazulla S, et al. Postsynaptic localization of gamma-aminobutyric acid transporters and receptors in the outer plexiform layer of the goldfish retina: An ultrastructural study. J Comp Neurol. 2004;474(1):58-74.
Klooster, J., Nunes Cardozo, B., Yazulla, S., & Kamermans, M. (2004). Postsynaptic localization of gamma-aminobutyric acid transporters and receptors in the outer plexiform layer of the goldfish retina: An ultrastructural study. The Journal of Comparative Neurology, 474(1), pp. 58-74.
Klooster J, et al. Postsynaptic Localization of Gamma-aminobutyric Acid Transporters and Receptors in the Outer Plexiform Layer of the Goldfish Retina: an Ultrastructural Study. J Comp Neurol. 2004 Jun 14;474(1):58-74. PubMed PMID: 15156579.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Postsynaptic localization of gamma-aminobutyric acid transporters and receptors in the outer plexiform layer of the goldfish retina: An ultrastructural study. AU - Klooster,Jan, AU - Nunes Cardozo,Bob, AU - Yazulla,Stephen, AU - Kamermans,Maarten, PY - 2004/5/25/pubmed PY - 2004/7/9/medline PY - 2004/5/25/entrez SP - 58 EP - 74 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 474 IS - 1 N2 - The gamma-aminobutyric acid (GABA)-ergic system in the outer plexiform layer (OPL) of the goldfish retina was studied via light and electron immunohistochemistry. The subcellular distributions of immunoreactivity (-IR) of plasma membrane GABA transporters GAT2 and GAT3, the alpha1 and alpha3 subunits of the ionotropic GABA(A) receptor, and the rho1 subunit of the ionotropic GABA(C) receptor were determined. The localization of the GAT2-IR and GAT3-IR to horizontal cell dendrites at the base of the cone synaptic complex was the main characteristic at the ultrastructural level. Very rarely, GAT2-IR and GAT3-IR were found in horizontal cell dendrites innervating rod spherules. alpha1-IR and alpha3-IR were seen in wide bands in the OPL, whereas rho1-IR appeared as a narrow band in the OPL. Most alpha1-IR was intracellular in rod and cone terminals. Membrane-associated alpha1-IR was observed in cone pedicles but not in rod spherules; postsynaptic elements were also labeled. alpha3-IR was concentrated in the lateral elements of horizontal cell dendrites in cone pedicles. In contrast, rho1-IR was found mainly on the spinules of the horizontal cell dendrites in cone pedicles. In addition, in another type of cone pedicle, rho1-IR was found at the position of OFF-bipolar cell dendrites. alpha3-IR and rho1-IR were rarely found in horizontal cell dendrites innervating rods. We suggest that two GABAergic pathways exist in the outer retina- first, a GABAergic positive loop with GABA receptors mainly on the horizontal cell dendrites and spinules and, second, a GABAergic feedback pathway involving GABA receptors on cone pedicles and GABA transporters on horizontal cells and that this pathway presumably modulates feedback strength from horizontal cells to cones. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/15156579/Postsynaptic_localization_of_gamma_aminobutyric_acid_transporters_and_receptors_in_the_outer_plexiform_layer_of_the_goldfish_retina:_An_ultrastructural_study_ L2 - https://doi.org/10.1002/cne.20114 DB - PRIME DP - Unbound Medicine ER -