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Transcriptional regulation in cowpea bruchid guts during adaptation to a plant defence protease inhibitor.
Insect Mol Biol. 2004 Jun; 13(3):283-91.IM

Abstract

Cowpea bruchid, when fed on a diet containing the soybean cysteine protease inhibitor soyacystatin N (scN), activates an array of counter-defence genes to adapt to the negative effects of the inhibitor and regain its normal rate of feeding and development. A collection of 1920 cDNAs was obtained by differential subtraction with cDNAs prepared from guts of the 4th instar larvae of scN-adapted (reared on scN-containing diet) and scN-unadapted (reared on regular scN-free diet) cowpea bruchids. Subsequent expression profiling using DNA microarray and Northern blot analyses identified ninety-four transcript species from this collection that are responsive to dietary scN. scN-adapted insects induced genes encoding protein and carbohydrate digestive enzymes, probably to help meet their carbon and nitrogen requirements. Up-regulation of antimicrobial and detoxification protein genes may represent a generalized defence response. Genes down-regulated by scN reflected physiological adjustments of the cowpea bruchids to scN challenge. A large portion of the responsive genes, presumably involved in carrying out the counter-defence response, were of unknown function. The full-length cDNA of an scN-inducible cathepsin B-like cysteine protease was obtained. Its transcriptional response to scN during larval development contrasts with the pattern of the cathepsin L family, the major digestive enzymes. These results suggest cathepsin B-like cysteine proteases may play a crucial role in cowpea bruchid adaptation to dietary scN.

Authors+Show Affiliations

Moon J
Department of Entomology, Texas A&M University, College Station, TX 77843, USA.
Salzman RA
No affiliation info available
Ahn JE
No affiliation info available
Koiwa H
No affiliation info available
Zhu-Salzman K
No affiliation info available

MeSH

Adaptation, PhysiologicalAmino Acid SequenceAnimalsBase SequenceBlotting, NorthernCathepsin BColeopteraCystatinsCysteine Proteinase InhibitorsDNA, ComplementaryDigestive SystemGene Expression ProfilingGene Expression RegulationLarvaMolecular Sequence DataOligonucleotide Array Sequence AnalysisSequence AlignmentSequence Analysis, DNASoybean Proteins

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15157229

Citation

Moon, J, et al. "Transcriptional Regulation in Cowpea Bruchid Guts During Adaptation to a Plant Defence Protease Inhibitor." Insect Molecular Biology, vol. 13, no. 3, 2004, pp. 283-91.
Moon J, Salzman RA, Ahn JE, et al. Transcriptional regulation in cowpea bruchid guts during adaptation to a plant defence protease inhibitor. Insect Mol Biol. 2004;13(3):283-91.
Moon, J., Salzman, R. A., Ahn, J. E., Koiwa, H., & Zhu-Salzman, K. (2004). Transcriptional regulation in cowpea bruchid guts during adaptation to a plant defence protease inhibitor. Insect Molecular Biology, 13(3), 283-91.
Moon J, et al. Transcriptional Regulation in Cowpea Bruchid Guts During Adaptation to a Plant Defence Protease Inhibitor. Insect Mol Biol. 2004;13(3):283-91. PubMed PMID: 15157229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional regulation in cowpea bruchid guts during adaptation to a plant defence protease inhibitor. AU - Moon,J, AU - Salzman,R A, AU - Ahn,J-E, AU - Koiwa,H, AU - Zhu-Salzman,K, PY - 2004/5/26/pubmed PY - 2004/7/21/medline PY - 2004/5/26/entrez SP - 283 EP - 91 JF - Insect molecular biology JO - Insect Mol Biol VL - 13 IS - 3 N2 - Cowpea bruchid, when fed on a diet containing the soybean cysteine protease inhibitor soyacystatin N (scN), activates an array of counter-defence genes to adapt to the negative effects of the inhibitor and regain its normal rate of feeding and development. A collection of 1920 cDNAs was obtained by differential subtraction with cDNAs prepared from guts of the 4th instar larvae of scN-adapted (reared on scN-containing diet) and scN-unadapted (reared on regular scN-free diet) cowpea bruchids. Subsequent expression profiling using DNA microarray and Northern blot analyses identified ninety-four transcript species from this collection that are responsive to dietary scN. scN-adapted insects induced genes encoding protein and carbohydrate digestive enzymes, probably to help meet their carbon and nitrogen requirements. Up-regulation of antimicrobial and detoxification protein genes may represent a generalized defence response. Genes down-regulated by scN reflected physiological adjustments of the cowpea bruchids to scN challenge. A large portion of the responsive genes, presumably involved in carrying out the counter-defence response, were of unknown function. The full-length cDNA of an scN-inducible cathepsin B-like cysteine protease was obtained. Its transcriptional response to scN during larval development contrasts with the pattern of the cathepsin L family, the major digestive enzymes. These results suggest cathepsin B-like cysteine proteases may play a crucial role in cowpea bruchid adaptation to dietary scN. SN - 0962-1075 UR - https://www.unboundmedicine.com/medline/citation/15157229/Transcriptional_regulation_in_cowpea_bruchid_guts_during_adaptation_to_a_plant_defence_protease_inhibitor_ DB - PRIME DP - Unbound Medicine ER -