Cell wall analysis.
Methods. 2004 Jul; 33(3):245-51.M

Abstract

The cell wall is a rigid structure essential for survival of the fungal cell. Because of its absence in mammalian cells, the cell wall is an attractive target for antifungal agents. Thus, for different reasons, it is important to know how the cell wall is synthesized and how different molecules regulate that synthesis. The Schizosaccharomyces pombe cell wall is mainly formed by glucose polysaccharides and some galactomannoproteins. Here, we describe a fast and reliable method to analyze changes in S. pombe cell wall composition by using specific enzymatic degradation and chemical treatment of purified cell walls. This approach provides a powerful means to analyze changes in (1,3)beta-glucan and (1,3)alpha-glucan, two main polysaccharides present in fungal cell walls. Analysis of cell wall polymers will be useful to search for new antifungal drugs that may inhibit cell wall biosynthesis and/or alter cell wall structure.

Authors+Show Affiliations

Pérez P

Instituto de Microbiología Bioquímica, Departamento de Microbiología y Genética, CSIC/Universidad de Salamanca, Spain. piper@usal.es

Ribas JC

No affiliation info available

MeSH

Cell WallSchizosaccharomyces

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15157892

Citation

Pérez, Pilar, and Juan C. Ribas. "Cell Wall Analysis." Methods (San Diego, Calif.), vol. 33, no. 3, 2004, pp. 245-51.

Pérez P, Ribas JC. Cell wall analysis. Methods. 2004;33(3):245-51.

Pérez, P., & Ribas, J. C. (2004). Cell wall analysis. Methods (San Diego, Calif.), 33(3), 245-51.

Pérez P, Ribas JC. Cell Wall Analysis. Methods. 2004;33(3):245-51. PubMed PMID: 15157892.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Cell wall analysis. AU - Pérez,Pilar, AU - Ribas,Juan C, PY - 2003/11/17/accepted PY - 2004/5/26/pubmed PY - 2005/1/8/medline PY - 2004/5/26/entrez SP - 245 EP - 51 JF - Methods (San Diego, Calif.) JO - Methods VL - 33 IS - 3 N2 - The cell wall is a rigid structure essential for survival of the fungal cell. Because of its absence in mammalian cells, the cell wall is an attractive target for antifungal agents. Thus, for different reasons, it is important to know how the cell wall is synthesized and how different molecules regulate that synthesis. The Schizosaccharomyces pombe cell wall is mainly formed by glucose polysaccharides and some galactomannoproteins. Here, we describe a fast and reliable method to analyze changes in S. pombe cell wall composition by using specific enzymatic degradation and chemical treatment of purified cell walls. This approach provides a powerful means to analyze changes in (1,3)beta-glucan and (1,3)alpha-glucan, two main polysaccharides present in fungal cell walls. Analysis of cell wall polymers will be useful to search for new antifungal drugs that may inhibit cell wall biosynthesis and/or alter cell wall structure. SN - 1046-2023 UR - https://www.unboundmedicine.com/medline/citation/15157892/Cell_wall_analysis_ DB - PRIME DP - Unbound Medicine ER -

Tags

Cell wall analysis.Methods. 2004 Jul; 33(3):245-51.M