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Acute reduction of circulating arginine in mice does not compromise whole body NO production.
Clin Nutr. 2004 Jun; 23(3):383-90.CN

Abstract

The amino acid arginine is the sole precursor for nitric oxide (NO) synthesis. We have now studied the role of acutely reducing circulating arginine on whole body NO production in mice. Measurements were performed in 4 groups of mice, treated with saline (SAL) or arginase (ASE), and SAL or bacterial endotoxin (LPS). After 5 h, a 57% reduction in circulating arginine was obtained by intravenous injections of arginase (SAL/SAL: 138+/-7; ASE/SAL: 59+/-10 microM, P<0.05). Reduced circulating arginine caused a reduction in plasma arginine flux (SAL/SAL: 82+/-6; ASE/SAL: 63+/-5 nmol/(10 g b.w. min), P<0.05), but did not change whole body NO production. LPS treatment caused an increase in NO production (SAL/SAL: 1.3+/-0.3 SAL/LPS 2.3+/-0.4 nmol/(10 g b.w. min), P<0.05), presumably by NOS-2 and was unaffected by reducing circulating arginine. Also, intestinal citrulline and renal arginine production were not increased in LPS-challenged mice with reduced circulating arginine levels. The present study indicates that an acute decrease in circulating arginine does not compromise whole body NO production and provides evidence against a role for renal arginine production to counteract an acute reduction of circulating arginine.

Authors+Show Affiliations

Department of Surgery, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands. mm_hallemeesch@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15158302

Citation

Hallemeesch, Marcella M., et al. "Acute Reduction of Circulating Arginine in Mice Does Not Compromise Whole Body NO Production." Clinical Nutrition (Edinburgh, Scotland), vol. 23, no. 3, 2004, pp. 383-90.
Hallemeesch MM, Vissers YL, Soeters PB, et al. Acute reduction of circulating arginine in mice does not compromise whole body NO production. Clin Nutr. 2004;23(3):383-90.
Hallemeesch, M. M., Vissers, Y. L., Soeters, P. B., & Deutz, N. E. (2004). Acute reduction of circulating arginine in mice does not compromise whole body NO production. Clinical Nutrition (Edinburgh, Scotland), 23(3), 383-90.
Hallemeesch MM, et al. Acute Reduction of Circulating Arginine in Mice Does Not Compromise Whole Body NO Production. Clin Nutr. 2004;23(3):383-90. PubMed PMID: 15158302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acute reduction of circulating arginine in mice does not compromise whole body NO production. AU - Hallemeesch,Marcella M, AU - Vissers,Yvonne L J, AU - Soeters,Peter B, AU - Deutz,Nicolaas E P, PY - 2003/05/23/received PY - 2003/09/01/accepted PY - 2004/5/26/pubmed PY - 2004/10/1/medline PY - 2004/5/26/entrez SP - 383 EP - 90 JF - Clinical nutrition (Edinburgh, Scotland) JO - Clin Nutr VL - 23 IS - 3 N2 - The amino acid arginine is the sole precursor for nitric oxide (NO) synthesis. We have now studied the role of acutely reducing circulating arginine on whole body NO production in mice. Measurements were performed in 4 groups of mice, treated with saline (SAL) or arginase (ASE), and SAL or bacterial endotoxin (LPS). After 5 h, a 57% reduction in circulating arginine was obtained by intravenous injections of arginase (SAL/SAL: 138+/-7; ASE/SAL: 59+/-10 microM, P<0.05). Reduced circulating arginine caused a reduction in plasma arginine flux (SAL/SAL: 82+/-6; ASE/SAL: 63+/-5 nmol/(10 g b.w. min), P<0.05), but did not change whole body NO production. LPS treatment caused an increase in NO production (SAL/SAL: 1.3+/-0.3 SAL/LPS 2.3+/-0.4 nmol/(10 g b.w. min), P<0.05), presumably by NOS-2 and was unaffected by reducing circulating arginine. Also, intestinal citrulline and renal arginine production were not increased in LPS-challenged mice with reduced circulating arginine levels. The present study indicates that an acute decrease in circulating arginine does not compromise whole body NO production and provides evidence against a role for renal arginine production to counteract an acute reduction of circulating arginine. SN - 0261-5614 UR - https://www.unboundmedicine.com/medline/citation/15158302/Acute_reduction_of_circulating_arginine_in_mice_does_not_compromise_whole_body_NO_production_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0261561403001973 DB - PRIME DP - Unbound Medicine ER -