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Sequential gene promoter interactions of C/EBPbeta, C/EBPalpha, and PPARgamma during adipogenesis.
Biochem Biophys Res Commun. 2004 Jun 18; 319(1):235-9.BB

Abstract

Treatment of 3T3-L1 preadipocytes with differentiation inducers triggers a cascade in which C/EBPbeta is rapidly expressed, followed by C/EBPalpha and PPARgamma. C/EBPalpha and PPARgamma then activate the expression of adipocyte genes that produce the differentiated phenotype. Circumstantial evidence indicates that C/EBPbeta activates transcription of the C/EBPalpha and PPARgamma genes, both of which possess C/EBP regulatory elements in their proximal promoters. Although C/EBPbeta is expressed immediately upon induction of differentiation, acquisition of DNA binding activity is delayed for approximately 14h. Chromatin immunoprecipitation (ChIP) analysis conducted 24h after induction revealed that C/EBPbeta binds to C/EBP regulatory elements in the proximal promoters of the C/EBPalpha and PPARgamma genes. After an additional delay ChIP analysis showed that C/EBPalpha binds to its own promoter and to the promoters of the PPARgamma and 422/aP2 genes. These findings support the view that once expressed, C/EBPalpha is responsible for maintaining the expression of PPARgamma and C/EBPalpha, as well as adipocyte proteins (e.g., 422/aP2) in the terminally differentiated state. Together these findings provide compelling evidence that C/EBPbeta, C/EBPalpha, and PPARgamma participate in a cascade during adipogenesis.

Authors+Show Affiliations

Tang QQ
Biological Chemistry, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, USA. qtang@jhem.jhmi.edu
Zhang JW
No affiliation info available
Daniel Lane M
No affiliation info available

MeSH

3T3-L1 CellsAdipocytesAdipose TissueAnimalsCCAAT-Enhancer-Binding Protein-alphaCCAAT-Enhancer-Binding Protein-betaCell DifferentiationChromatinDNAImmunoblottingMicePhenotypePrecipitin TestsPromoter Regions, GeneticProtein BindingReceptors, Cytoplasmic and NuclearTime FactorsTranscription FactorsTranscription, Genetic

Pub Type(s)

Duplicate Publication
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15158467

Citation

Tang, Qi-Qun, et al. "Sequential Gene Promoter Interactions of C/EBPbeta, C/EBPalpha, and PPARgamma During Adipogenesis." Biochemical and Biophysical Research Communications, vol. 319, no. 1, 2004, pp. 235-9.
Tang QQ, Zhang JW, Daniel Lane M. Sequential gene promoter interactions of C/EBPbeta, C/EBPalpha, and PPARgamma during adipogenesis. Biochem Biophys Res Commun. 2004;319(1):235-9.
Tang, Q. Q., Zhang, J. W., & Daniel Lane, M. (2004). Sequential gene promoter interactions of C/EBPbeta, C/EBPalpha, and PPARgamma during adipogenesis. Biochemical and Biophysical Research Communications, 319(1), 235-9.
Tang QQ, Zhang JW, Daniel Lane M. Sequential Gene Promoter Interactions of C/EBPbeta, C/EBPalpha, and PPARgamma During Adipogenesis. Biochem Biophys Res Commun. 2004 Jun 18;319(1):235-9. PubMed PMID: 15158467.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequential gene promoter interactions of C/EBPbeta, C/EBPalpha, and PPARgamma during adipogenesis. AU - Tang,Qi-Qun, AU - Zhang,Jiang-Wen, AU - Daniel Lane,M, PY - 2004/03/31/received PY - 2004/5/26/pubmed PY - 2004/7/13/medline PY - 2004/5/26/entrez SP - 235 EP - 9 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 319 IS - 1 N2 - Treatment of 3T3-L1 preadipocytes with differentiation inducers triggers a cascade in which C/EBPbeta is rapidly expressed, followed by C/EBPalpha and PPARgamma. C/EBPalpha and PPARgamma then activate the expression of adipocyte genes that produce the differentiated phenotype. Circumstantial evidence indicates that C/EBPbeta activates transcription of the C/EBPalpha and PPARgamma genes, both of which possess C/EBP regulatory elements in their proximal promoters. Although C/EBPbeta is expressed immediately upon induction of differentiation, acquisition of DNA binding activity is delayed for approximately 14h. Chromatin immunoprecipitation (ChIP) analysis conducted 24h after induction revealed that C/EBPbeta binds to C/EBP regulatory elements in the proximal promoters of the C/EBPalpha and PPARgamma genes. After an additional delay ChIP analysis showed that C/EBPalpha binds to its own promoter and to the promoters of the PPARgamma and 422/aP2 genes. These findings support the view that once expressed, C/EBPalpha is responsible for maintaining the expression of PPARgamma and C/EBPalpha, as well as adipocyte proteins (e.g., 422/aP2) in the terminally differentiated state. Together these findings provide compelling evidence that C/EBPbeta, C/EBPalpha, and PPARgamma participate in a cascade during adipogenesis. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/15158467/Sequential_gene_promoter_interactions_of_C/EBPbeta_C/EBPalpha_and_PPARgamma_during_adipogenesis_ DB - PRIME DP - Unbound Medicine ER -