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Androgen receptor mRNA expression in the bovine ovary.
Domest Anim Endocrinol. 2004 Jul; 27(1):81-8.DA

Abstract

Previous studies have shown that androgen receptor (AR) is expressed in granulosa cells of healthy, growing ovarian follicles in rats and primates. However, AR expression in the bovine ovary has not been examined. Therefore, a 346-base pair segment of the bovine AR was cloned and sequenced. Using a ribonuclease protection assay, AR expression was detected in total RNA from bovine ovarian cortex. Expression (absence or presence) of AR mRNA was detected by in situ hybridization in bovine ovarian cortex. Follicles (n = 32) were classified as follows: type 1 (1 layer of flattened granulosa cells), type 2 (1-1.5 layers of cuboidal granulosa cells), type 3 (2-3 layers of granulosa cells), type 4 (4-6 layers of cuboidal granulosa cells and formation of thecal layer), and type 5 (>6 layers of cuboidal granulosa cells, defined theca layer, and antrum formation). Frequency of AR mRNA expression increased (P < 0.001) as follicles entered the growing pool. Expression of AR mRNA was absent in type 1 follicles (n = 8), but present in the granulosa cells of 41% of type 2 follicles (n = 12). In types 3-5 follicles, AR mRNA expression was present in granulosa cells of 100% of follicles examined (n = 4, 4, and 4, respectively) and was greater than type 1 follicles (P = 0.002). These data provide evidence of AR mRNA expression in bovine follicles and suggest that AR mRNA increases during early follicle development.

Authors+Show Affiliations

Department of Animal Sciences, 163 Animal Science Research Center, University of Missouri, East Campus Dr., Columbia, MO 65211, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15158536

Citation

Hampton, J H., et al. "Androgen Receptor mRNA Expression in the Bovine Ovary." Domestic Animal Endocrinology, vol. 27, no. 1, 2004, pp. 81-8.
Hampton JH, Manikkam M, Lubahn DB, et al. Androgen receptor mRNA expression in the bovine ovary. Domest Anim Endocrinol. 2004;27(1):81-8.
Hampton, J. H., Manikkam, M., Lubahn, D. B., Smith, M. F., & Garverick, H. A. (2004). Androgen receptor mRNA expression in the bovine ovary. Domestic Animal Endocrinology, 27(1), 81-8.
Hampton JH, et al. Androgen Receptor mRNA Expression in the Bovine Ovary. Domest Anim Endocrinol. 2004;27(1):81-8. PubMed PMID: 15158536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Androgen receptor mRNA expression in the bovine ovary. AU - Hampton,J H, AU - Manikkam,M, AU - Lubahn,D B, AU - Smith,M F, AU - Garverick,H A, PY - 2003/11/21/received PY - 2004/01/19/accepted PY - 2004/5/26/pubmed PY - 2004/8/4/medline PY - 2004/5/26/entrez SP - 81 EP - 8 JF - Domestic animal endocrinology JO - Domest Anim Endocrinol VL - 27 IS - 1 N2 - Previous studies have shown that androgen receptor (AR) is expressed in granulosa cells of healthy, growing ovarian follicles in rats and primates. However, AR expression in the bovine ovary has not been examined. Therefore, a 346-base pair segment of the bovine AR was cloned and sequenced. Using a ribonuclease protection assay, AR expression was detected in total RNA from bovine ovarian cortex. Expression (absence or presence) of AR mRNA was detected by in situ hybridization in bovine ovarian cortex. Follicles (n = 32) were classified as follows: type 1 (1 layer of flattened granulosa cells), type 2 (1-1.5 layers of cuboidal granulosa cells), type 3 (2-3 layers of granulosa cells), type 4 (4-6 layers of cuboidal granulosa cells and formation of thecal layer), and type 5 (>6 layers of cuboidal granulosa cells, defined theca layer, and antrum formation). Frequency of AR mRNA expression increased (P < 0.001) as follicles entered the growing pool. Expression of AR mRNA was absent in type 1 follicles (n = 8), but present in the granulosa cells of 41% of type 2 follicles (n = 12). In types 3-5 follicles, AR mRNA expression was present in granulosa cells of 100% of follicles examined (n = 4, 4, and 4, respectively) and was greater than type 1 follicles (P = 0.002). These data provide evidence of AR mRNA expression in bovine follicles and suggest that AR mRNA increases during early follicle development. SN - 0739-7240 UR - https://www.unboundmedicine.com/medline/citation/15158536/Androgen_receptor_mRNA_expression_in_the_bovine_ovary_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0739724004000268 DB - PRIME DP - Unbound Medicine ER -