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Polyphenolic compounds, antioxidant capacity, and quinone reductase activity of an aqueous extract of Ardisia compressa in comparison to mate (Ilex paraguariensis) and green (Camellia sinensis) teas.
J Agric Food Chem. 2004 Jun 02; 52(11):3583-9.JA

Abstract

Aqueous extracts of the leaves of Ardisia compressa (AC) have been used in folk medicine to treat various liver disorders including liver cancer. The objective of this study was to partially characterize and determine the total polyphenol content, antioxidant capacity, and quinone reductase activity of A. compressa tea in comparison to mate (Ilex paraguariensis, MT) and green (Camellia sinensis,GT) teas. Total polyphenol content, antioxidant capacity, and phase II enzyme induction capacity were measured by the modified Folin-Ciocalteu, ORAC, and quinone reductase (QR) assays, respectively. The major polyphenols in AC were not catechins. HPLC retention times and standard spikes of AC indicated the presence of gallic acid, epicatechin gallate, ardisin and kaempferol. Using catechin as standard, the total polyphenol value of AC (36.8 +/- 1.1 mg/mg DL) was significantly lower than GT (137.2 +/- 5.8 mg equivalent of (+)-catechin/mg dried leaves, DL) and MT (82.1 +/- 3.8 mg/mg DL) (P < 0.001). Antioxidant capacity (AC, 333; GT, 1346; MT, 1239 mmol Trolox equivalents/g DL) correlated with total polyphenol values (r(2) = 0.86, P < 0.01). AC (4.5-12.5 microg/mL) induced QR enzyme, in Hepa1c1c7 cells, up to 15%. MT and GT showed no induction at the concentrations tested (0.5-10.5 and 0.5-12.5 mg/mL, respectively). These results suggest that AC has a different mechanism of protection against cytotoxicity that is not related to its antioxidant capacity. Further studies are needed to determine such mechanisms and to explore its potential as a chemopreventive or therapeutic agent.

Authors+Show Affiliations

Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 228 ERML, MC-051, 1201 W Gregory Drive, Urbana, Illinois 61801, USA.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15161234

Citation

Chandra, Sonia, and Elvira De Mejia Gonzalez. "Polyphenolic Compounds, Antioxidant Capacity, and Quinone Reductase Activity of an Aqueous Extract of Ardisia Compressa in Comparison to Mate (Ilex Paraguariensis) and Green (Camellia Sinensis) Teas." Journal of Agricultural and Food Chemistry, vol. 52, no. 11, 2004, pp. 3583-9.
Chandra S, De Mejia Gonzalez E. Polyphenolic compounds, antioxidant capacity, and quinone reductase activity of an aqueous extract of Ardisia compressa in comparison to mate (Ilex paraguariensis) and green (Camellia sinensis) teas. J Agric Food Chem. 2004;52(11):3583-9.
Chandra, S., & De Mejia Gonzalez, E. (2004). Polyphenolic compounds, antioxidant capacity, and quinone reductase activity of an aqueous extract of Ardisia compressa in comparison to mate (Ilex paraguariensis) and green (Camellia sinensis) teas. Journal of Agricultural and Food Chemistry, 52(11), 3583-9.
Chandra S, De Mejia Gonzalez E. Polyphenolic Compounds, Antioxidant Capacity, and Quinone Reductase Activity of an Aqueous Extract of Ardisia Compressa in Comparison to Mate (Ilex Paraguariensis) and Green (Camellia Sinensis) Teas. J Agric Food Chem. 2004 Jun 2;52(11):3583-9. PubMed PMID: 15161234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyphenolic compounds, antioxidant capacity, and quinone reductase activity of an aqueous extract of Ardisia compressa in comparison to mate (Ilex paraguariensis) and green (Camellia sinensis) teas. AU - Chandra,Sonia, AU - De Mejia Gonzalez,Elvira, PY - 2004/5/27/pubmed PY - 2004/7/10/medline PY - 2004/5/27/entrez SP - 3583 EP - 9 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 52 IS - 11 N2 - Aqueous extracts of the leaves of Ardisia compressa (AC) have been used in folk medicine to treat various liver disorders including liver cancer. The objective of this study was to partially characterize and determine the total polyphenol content, antioxidant capacity, and quinone reductase activity of A. compressa tea in comparison to mate (Ilex paraguariensis, MT) and green (Camellia sinensis,GT) teas. Total polyphenol content, antioxidant capacity, and phase II enzyme induction capacity were measured by the modified Folin-Ciocalteu, ORAC, and quinone reductase (QR) assays, respectively. The major polyphenols in AC were not catechins. HPLC retention times and standard spikes of AC indicated the presence of gallic acid, epicatechin gallate, ardisin and kaempferol. Using catechin as standard, the total polyphenol value of AC (36.8 +/- 1.1 mg/mg DL) was significantly lower than GT (137.2 +/- 5.8 mg equivalent of (+)-catechin/mg dried leaves, DL) and MT (82.1 +/- 3.8 mg/mg DL) (P < 0.001). Antioxidant capacity (AC, 333; GT, 1346; MT, 1239 mmol Trolox equivalents/g DL) correlated with total polyphenol values (r(2) = 0.86, P < 0.01). AC (4.5-12.5 microg/mL) induced QR enzyme, in Hepa1c1c7 cells, up to 15%. MT and GT showed no induction at the concentrations tested (0.5-10.5 and 0.5-12.5 mg/mL, respectively). These results suggest that AC has a different mechanism of protection against cytotoxicity that is not related to its antioxidant capacity. Further studies are needed to determine such mechanisms and to explore its potential as a chemopreventive or therapeutic agent. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15161234/Polyphenolic_compounds_antioxidant_capacity_and_quinone_reductase_activity_of_an_aqueous_extract_of_Ardisia_compressa_in_comparison_to_mate__Ilex_paraguariensis__and_green__Camellia_sinensis__teas_ L2 - https://dx.doi.org/10.1021/jf0352632 DB - PRIME DP - Unbound Medicine ER -