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Alkali extraction of beta-d-glucans from Saccharomyces cerevisiae cell wall and study of their adsorptive properties toward zearalenone.
J Agric Food Chem. 2004 Jun 02; 52(11):3666-73.JA

Abstract

The isolated cell wall of Saccharomyces cerevisiae has some capacity to adsorb zearalenone (affinity near 30%) and reduce the bioavailability of toxins in the digestive tract. The adsorption process was quantified in vitro, and the data obtained when plotted with Hill's equation indicated a cooperative process. The model showed that the adsorption capacity was related to the yeast cell wall composition. This work focused on the role of various beta-d-glucan types in the efficacy of zearalenone adsorption by yeast cell wall and sought to elucidate some of the adsorption mechanisms. Zearalenone was mixed at 37 degrees C with a constant quantity of alkali-soluble or alkali-insoluble beta-d-glucans isolated from yeast cell walls, and the amount of adsorbed zearalenone was measured. Given that the alkali solubility of beta-d-glucans is a determining factor for their three-dimensional conformation and that the alkali-insoluble fraction had a greater affinity (up to 50%) than the alkali-soluble fraction (approximately 16%), it was concluded that the three-dimensional structure strongly influences the adsorption process. The alkali insolubility of beta-d-glucans led to the formation of single and/or triple helices, which have been identified as the most favorable structures for zearalenone adsorption efficacy. The beta(1,3)-d-glucan and beta(1,6)-d-glucan compositions of the two alkali-extracted fractions and their involvement in the adsorption process are discussed.

Authors+Show Affiliations

Yiannikouris A
UR1053 INRA, Unité de Recherches sur les Herbivores Centre de Clermont-Theix, 63122 St-Genès Champanelle, France.
François J
No affiliation info available
Poughon L
No affiliation info available
Dussap CG
No affiliation info available
Bertin G
No affiliation info available
Jeminet G
No affiliation info available
Jouany JP
No affiliation info available

MeSH

AdsorptionCell WallGlucansHydrogen-Ion ConcentrationSaccharomyces cerevisiaeSolubilityZearalenone

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15161247

Citation

Yiannikouris, Alexandros, et al. "Alkali Extraction of Beta-d-glucans From Saccharomyces Cerevisiae Cell Wall and Study of Their Adsorptive Properties Toward Zearalenone." Journal of Agricultural and Food Chemistry, vol. 52, no. 11, 2004, pp. 3666-73.
Yiannikouris A, François J, Poughon L, et al. Alkali extraction of beta-d-glucans from Saccharomyces cerevisiae cell wall and study of their adsorptive properties toward zearalenone. J Agric Food Chem. 2004;52(11):3666-73.
Yiannikouris, A., François, J., Poughon, L., Dussap, C. G., Bertin, G., Jeminet, G., & Jouany, J. P. (2004). Alkali extraction of beta-d-glucans from Saccharomyces cerevisiae cell wall and study of their adsorptive properties toward zearalenone. Journal of Agricultural and Food Chemistry, 52(11), 3666-73.
Yiannikouris A, et al. Alkali Extraction of Beta-d-glucans From Saccharomyces Cerevisiae Cell Wall and Study of Their Adsorptive Properties Toward Zearalenone. J Agric Food Chem. 2004 Jun 2;52(11):3666-73. PubMed PMID: 15161247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alkali extraction of beta-d-glucans from Saccharomyces cerevisiae cell wall and study of their adsorptive properties toward zearalenone. AU - Yiannikouris,Alexandros, AU - François,Jean, AU - Poughon,Laurent, AU - Dussap,Claude-Gilles, AU - Bertin,Gérard, AU - Jeminet,Georges, AU - Jouany,Jean-Pierre, PY - 2004/5/27/pubmed PY - 2004/7/10/medline PY - 2004/5/27/entrez SP - 3666 EP - 73 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 52 IS - 11 N2 - The isolated cell wall of Saccharomyces cerevisiae has some capacity to adsorb zearalenone (affinity near 30%) and reduce the bioavailability of toxins in the digestive tract. The adsorption process was quantified in vitro, and the data obtained when plotted with Hill's equation indicated a cooperative process. The model showed that the adsorption capacity was related to the yeast cell wall composition. This work focused on the role of various beta-d-glucan types in the efficacy of zearalenone adsorption by yeast cell wall and sought to elucidate some of the adsorption mechanisms. Zearalenone was mixed at 37 degrees C with a constant quantity of alkali-soluble or alkali-insoluble beta-d-glucans isolated from yeast cell walls, and the amount of adsorbed zearalenone was measured. Given that the alkali solubility of beta-d-glucans is a determining factor for their three-dimensional conformation and that the alkali-insoluble fraction had a greater affinity (up to 50%) than the alkali-soluble fraction (approximately 16%), it was concluded that the three-dimensional structure strongly influences the adsorption process. The alkali insolubility of beta-d-glucans led to the formation of single and/or triple helices, which have been identified as the most favorable structures for zearalenone adsorption efficacy. The beta(1,3)-d-glucan and beta(1,6)-d-glucan compositions of the two alkali-extracted fractions and their involvement in the adsorption process are discussed. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15161247/Alkali_extraction_of_beta_d_glucans_from_Saccharomyces_cerevisiae_cell_wall_and_study_of_their_adsorptive_properties_toward_zearalenone_ DB - PRIME DP - Unbound Medicine ER -