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Biocompatibility and biodegradation of a bone composite containing tricalcium phosphate and genipin crosslinked gelatin.
J Biomed Mater Res A. 2004 Jun 15; 69(4):709-17.JB

Abstract

A biodegradable composite (GGT) containing tricalcium phosphate ceramic particles and genipin crosslinked gelatin was developed for use as a bone substitute. The objective of this study was to assess the biocompatibility and the osteoconductivity of the GGT composite on new bone formation in vitro. Additionally, biodegradation and biocompatibility of the GGT composite in animals were investigated. Results of the GGT composites cocultured with osteoblasts showed that the concentration of genipin used as a crosslinking agent should be <0.5 wt % to avoid cytotoxicity. For in vivo degradation studies, we found that when the concentration of genipin in the composite <0.5 wt % was not enough to fully crosslink the gelatin, it results in a rapid degradation of the gelatin-genipin mixture. However, we also found that the foreign body capsule surrounding the GGT composite containing 1.0 wt % of the genipin was much thicker than that in the other three groups, that is, the composites containing 0.05, 0.1, and 0.5 wt % of the genipin. We therefore concluded that the ideal concentration of genipin used in the GGT was 0.5 wt %. Finally, we examined the organ culture units, which were maintained in cultured medium for 5 weeks. Morphology of tissue was observed and the quantitative evaluation of the regenerated bone was determined. We found that the GGT composites containing 0.5 wt % of the genipin had an excellent biocompatibility and could produce osteoconduction for the regenerating bone tissues.

Authors+Show Affiliations

Department of Radiological Technology, Chungtai Institute of Health Science and Technology, Taichung, Taiwan. chyao@ctc.edu.twNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15162413

Citation

Yao, Chun-Hsu, et al. "Biocompatibility and Biodegradation of a Bone Composite Containing Tricalcium Phosphate and Genipin Crosslinked Gelatin." Journal of Biomedical Materials Research. Part A, vol. 69, no. 4, 2004, pp. 709-17.
Yao CH, Liu BS, Hsu SH, et al. Biocompatibility and biodegradation of a bone composite containing tricalcium phosphate and genipin crosslinked gelatin. J Biomed Mater Res A. 2004;69(4):709-17.
Yao, C. H., Liu, B. S., Hsu, S. H., Chen, Y. S., & Tsai, C. C. (2004). Biocompatibility and biodegradation of a bone composite containing tricalcium phosphate and genipin crosslinked gelatin. Journal of Biomedical Materials Research. Part A, 69(4), 709-17.
Yao CH, et al. Biocompatibility and Biodegradation of a Bone Composite Containing Tricalcium Phosphate and Genipin Crosslinked Gelatin. J Biomed Mater Res A. 2004 Jun 15;69(4):709-17. PubMed PMID: 15162413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biocompatibility and biodegradation of a bone composite containing tricalcium phosphate and genipin crosslinked gelatin. AU - Yao,Chun-Hsu, AU - Liu,Bai-Shuan, AU - Hsu,Shan-Hui, AU - Chen,Yueh-Sheng, AU - Tsai,Chin-Chuan, PY - 2004/5/27/pubmed PY - 2004/12/31/medline PY - 2004/5/27/entrez SP - 709 EP - 17 JF - Journal of biomedical materials research. Part A JO - J Biomed Mater Res A VL - 69 IS - 4 N2 - A biodegradable composite (GGT) containing tricalcium phosphate ceramic particles and genipin crosslinked gelatin was developed for use as a bone substitute. The objective of this study was to assess the biocompatibility and the osteoconductivity of the GGT composite on new bone formation in vitro. Additionally, biodegradation and biocompatibility of the GGT composite in animals were investigated. Results of the GGT composites cocultured with osteoblasts showed that the concentration of genipin used as a crosslinking agent should be <0.5 wt % to avoid cytotoxicity. For in vivo degradation studies, we found that when the concentration of genipin in the composite <0.5 wt % was not enough to fully crosslink the gelatin, it results in a rapid degradation of the gelatin-genipin mixture. However, we also found that the foreign body capsule surrounding the GGT composite containing 1.0 wt % of the genipin was much thicker than that in the other three groups, that is, the composites containing 0.05, 0.1, and 0.5 wt % of the genipin. We therefore concluded that the ideal concentration of genipin used in the GGT was 0.5 wt %. Finally, we examined the organ culture units, which were maintained in cultured medium for 5 weeks. Morphology of tissue was observed and the quantitative evaluation of the regenerated bone was determined. We found that the GGT composites containing 0.5 wt % of the genipin had an excellent biocompatibility and could produce osteoconduction for the regenerating bone tissues. SN - 1549-3296 UR - https://www.unboundmedicine.com/medline/citation/15162413/Biocompatibility_and_biodegradation_of_a_bone_composite_containing_tricalcium_phosphate_and_genipin_crosslinked_gelatin_ L2 - https://doi.org/10.1002/jbm.a.30045 DB - PRIME DP - Unbound Medicine ER -