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The involvement of p53 in paraquat-induced apoptosis in human lung epithelial-like cells.
Int J Toxicol. 2004 Jan-Feb; 23(1):33-40.IJ

Abstract

To investigate the possible role of p53 in the progression of paraquat-induced apoptosis, the authors used two cell lines that were wild-type p53-expressing human lung epithelial-like cell line (L132) and a p53-deficient human promyelocytic leukemia cell line (U937) and explored the linkage between p53, DNA damage, and apoptosis. Following paraquat exposure to L132 cells, the percentage of S-phase cells decreased significantly and the expression of p53 protein increased, suggesting that entry into S phase from G1 phase was blocked. U937 cells showed complete resistance to paraquat, although paraquat-evoked initial single-stranded DNA breaks was shown equally in either L132 or U937 cells, as assessed by single-cell gel electrophoresis. U937 and L132 cells die normally with similar kinetics when exposed to tumor necrosis factor in the presence of cycloheximide, indicating that their capacity to undergo p53-independent mechanisms of inducing apoptosis has an equal rate. These results suggest that paraquat-induced DNA damage caused G1 arrest and apoptosis only in L132 cells, and that p53 protein accumulation is required for the induction of apoptosis by paraquat.

Authors+Show Affiliations

Department of Emergency and Critical Care Medicine, Kansai Medical University, Osaka, Japan. takeyama@takii.kmu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15162845

Citation

Takeyama, Naoshi, et al. "The Involvement of P53 in Paraquat-induced Apoptosis in Human Lung Epithelial-like Cells." International Journal of Toxicology, vol. 23, no. 1, 2004, pp. 33-40.
Takeyama N, Tanaka T, Yabuki T, et al. The involvement of p53 in paraquat-induced apoptosis in human lung epithelial-like cells. Int J Toxicol. 2004;23(1):33-40.
Takeyama, N., Tanaka, T., Yabuki, T., & Nakatani, T. (2004). The involvement of p53 in paraquat-induced apoptosis in human lung epithelial-like cells. International Journal of Toxicology, 23(1), 33-40.
Takeyama N, et al. The Involvement of P53 in Paraquat-induced Apoptosis in Human Lung Epithelial-like Cells. Int J Toxicol. 2004 Jan-Feb;23(1):33-40. PubMed PMID: 15162845.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The involvement of p53 in paraquat-induced apoptosis in human lung epithelial-like cells. AU - Takeyama,Naoshi, AU - Tanaka,Takaya, AU - Yabuki,Teru, AU - Nakatani,Toshio, PY - 2004/5/28/pubmed PY - 2004/9/29/medline PY - 2004/5/28/entrez SP - 33 EP - 40 JF - International journal of toxicology JO - Int J Toxicol VL - 23 IS - 1 N2 - To investigate the possible role of p53 in the progression of paraquat-induced apoptosis, the authors used two cell lines that were wild-type p53-expressing human lung epithelial-like cell line (L132) and a p53-deficient human promyelocytic leukemia cell line (U937) and explored the linkage between p53, DNA damage, and apoptosis. Following paraquat exposure to L132 cells, the percentage of S-phase cells decreased significantly and the expression of p53 protein increased, suggesting that entry into S phase from G1 phase was blocked. U937 cells showed complete resistance to paraquat, although paraquat-evoked initial single-stranded DNA breaks was shown equally in either L132 or U937 cells, as assessed by single-cell gel electrophoresis. U937 and L132 cells die normally with similar kinetics when exposed to tumor necrosis factor in the presence of cycloheximide, indicating that their capacity to undergo p53-independent mechanisms of inducing apoptosis has an equal rate. These results suggest that paraquat-induced DNA damage caused G1 arrest and apoptosis only in L132 cells, and that p53 protein accumulation is required for the induction of apoptosis by paraquat. SN - 1091-5818 UR - https://www.unboundmedicine.com/medline/citation/15162845/The_involvement_of_p53_in_paraquat_induced_apoptosis_in_human_lung_epithelial_like_cells_ L2 - https://journals.sagepub.com/doi/10.1080/10915810490265432?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -