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Surveillance of influenza isolates for susceptibility to neuraminidase inhibitors during the 2000-2002 influenza seasons.

Abstract

Neuraminidase (NA) inhibitors (NI) have recently been licensed for the prophylaxis and treatment of influenza virus infection in humans. This study has utilized a new chemiluminescent (CL) neuraminidase assay to routinely monitor more than a thousand influenza field isolates collected worldwide during the 2000-2002 seasons for susceptibility to both licensed NIs, zanamivir, and oseltamivir by determining the 50% inhibitory concentration (IC50). Our data demonstrated that influenza A viruses of the N2 subtype were less susceptible to zanamivir, but not oseltamivir, than those of the N1 subtype such that 41 of 45 confirmed H1N2 isolates could be reliably differentiated from H1N1 viruses based on their zanamivir susceptibility. Pre-titration of influenza A viruses appeared to have no effect on IC50 determined for either NI, while pre-titration of influenza B viruses significantly reduced oseltamivir IC50 and increased zanamivir IC50. Influenza B viruses were less susceptible to either compound than type A isolates. The CL assay is a rapid and reliable method for screening large numbers of influenza isolates for NI susceptibility. Reassortant viruses of the H1N2 subtype that started to circulate worldwide during the 2001-2002 season can be reliably separated from H1N1 viruses based on their zanamivir susceptibility, enabling large scale screening of H1 isolates for determining the prevalence of such reassortants.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Influenza Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Mailstop G-16, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.

    ,

    Source

    Virus research 103:1-2 2004 Jul pg 195-7

    MeSH

    Acetamides
    Antiviral Agents
    Enzyme Inhibitors
    Guanidines
    Humans
    Influenza A virus
    Influenza B virus
    Influenza, Human
    Luminescent Measurements
    Microbial Sensitivity Tests
    Neuraminidase
    Oseltamivir
    Population Surveillance
    Pyrans
    Sialic Acids
    Zanamivir

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    15163509

    Citation

    Mungall, Bruce A., et al. "Surveillance of Influenza Isolates for Susceptibility to Neuraminidase Inhibitors During the 2000-2002 Influenza Seasons." Virus Research, vol. 103, no. 1-2, 2004, pp. 195-7.
    Mungall BA, Xu X, Klimov A. Surveillance of influenza isolates for susceptibility to neuraminidase inhibitors during the 2000-2002 influenza seasons. Virus Res. 2004;103(1-2):195-7.
    Mungall, B. A., Xu, X., & Klimov, A. (2004). Surveillance of influenza isolates for susceptibility to neuraminidase inhibitors during the 2000-2002 influenza seasons. Virus Research, 103(1-2), pp. 195-7.
    Mungall BA, Xu X, Klimov A. Surveillance of Influenza Isolates for Susceptibility to Neuraminidase Inhibitors During the 2000-2002 Influenza Seasons. Virus Res. 2004;103(1-2):195-7. PubMed PMID: 15163509.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Surveillance of influenza isolates for susceptibility to neuraminidase inhibitors during the 2000-2002 influenza seasons. AU - Mungall,Bruce A, AU - Xu,Xiyan, AU - Klimov,Alexander, PY - 2004/5/28/pubmed PY - 2004/9/24/medline PY - 2004/5/28/entrez SP - 195 EP - 7 JF - Virus research JO - Virus Res. VL - 103 IS - 1-2 N2 - Neuraminidase (NA) inhibitors (NI) have recently been licensed for the prophylaxis and treatment of influenza virus infection in humans. This study has utilized a new chemiluminescent (CL) neuraminidase assay to routinely monitor more than a thousand influenza field isolates collected worldwide during the 2000-2002 seasons for susceptibility to both licensed NIs, zanamivir, and oseltamivir by determining the 50% inhibitory concentration (IC50). Our data demonstrated that influenza A viruses of the N2 subtype were less susceptible to zanamivir, but not oseltamivir, than those of the N1 subtype such that 41 of 45 confirmed H1N2 isolates could be reliably differentiated from H1N1 viruses based on their zanamivir susceptibility. Pre-titration of influenza A viruses appeared to have no effect on IC50 determined for either NI, while pre-titration of influenza B viruses significantly reduced oseltamivir IC50 and increased zanamivir IC50. Influenza B viruses were less susceptible to either compound than type A isolates. The CL assay is a rapid and reliable method for screening large numbers of influenza isolates for NI susceptibility. Reassortant viruses of the H1N2 subtype that started to circulate worldwide during the 2001-2002 season can be reliably separated from H1N1 viruses based on their zanamivir susceptibility, enabling large scale screening of H1 isolates for determining the prevalence of such reassortants. SN - 0168-1702 UR - https://www.unboundmedicine.com/medline/citation/15163509/Surveillance_of_influenza_isolates_for_susceptibility_to_neuraminidase_inhibitors_during_the_2000_2002_influenza_seasons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168170204001327 DB - PRIME DP - Unbound Medicine ER -