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Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions.
J Biotechnol. 2004 Jun 10; 110(3):287-93.JB

Abstract

A combined bioreactor system, composed of a stirred tank and a three-stage tubular bioreactor in series and with a total working volume of 3260 ml, was established. Continuous ethanol production was carried out using Saccharomyces cerevisiae and a very high gravity (VHG) medium containing 280 g l(-1) glucose. An average ethanol concentration of 124.6 g l(-1) or 15.8% (v) was produced when the bioreactor system was operated at a dilution rate of 0.012 h(-1). The yield of ethanol to glucose consumed was calculated to be 0.484 or 94.7% of its theoretical value of 0.511 when ethanol entrapped in the exhaust gas was incorporated. Meanwhile, quasi-steady states and non-steady oscillations were observed for residual glucose, ethanol and biomass concentrations for all of these bioreactors during their operations. Models that can be used to predict yeast cell lysis and viability loss were developed.

Authors+Show Affiliations

Bai FW
Department of Chemical Engineering, University of Waterloo, Waterloo, Ont., Canada N2L 3G1. fwbai@dlut.edu.cn
Chen LJ
No affiliation info available
Zhang Z
No affiliation info available
Anderson WA
No affiliation info available
Moo-Young M
No affiliation info available

MeSH

BiomassBioreactorsCell Physiological PhenomenaCulture MediaEthanolFermentationGlucoseGravitationKineticsModels, BiologicalPeptonesSaccharomyces cerevisiae

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15163519

Citation

Bai, F W., et al. "Continuous Ethanol Production and Evaluation of Yeast Cell Lysis and Viability Loss Under Very High Gravity Medium Conditions." Journal of Biotechnology, vol. 110, no. 3, 2004, pp. 287-93.
Bai FW, Chen LJ, Zhang Z, et al. Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions. J Biotechnol. 2004;110(3):287-93.
Bai, F. W., Chen, L. J., Zhang, Z., Anderson, W. A., & Moo-Young, M. (2004). Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions. Journal of Biotechnology, 110(3), 287-93.
Bai FW, et al. Continuous Ethanol Production and Evaluation of Yeast Cell Lysis and Viability Loss Under Very High Gravity Medium Conditions. J Biotechnol. 2004 Jun 10;110(3):287-93. PubMed PMID: 15163519.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions. AU - Bai,F W, AU - Chen,L J, AU - Zhang,Z, AU - Anderson,W A, AU - Moo-Young,M, PY - 2003/08/12/received PY - 2003/12/16/revised PY - 2004/01/19/accepted PY - 2004/5/28/pubmed PY - 2004/12/16/medline PY - 2004/5/28/entrez SP - 287 EP - 93 JF - Journal of biotechnology JO - J Biotechnol VL - 110 IS - 3 N2 - A combined bioreactor system, composed of a stirred tank and a three-stage tubular bioreactor in series and with a total working volume of 3260 ml, was established. Continuous ethanol production was carried out using Saccharomyces cerevisiae and a very high gravity (VHG) medium containing 280 g l(-1) glucose. An average ethanol concentration of 124.6 g l(-1) or 15.8% (v) was produced when the bioreactor system was operated at a dilution rate of 0.012 h(-1). The yield of ethanol to glucose consumed was calculated to be 0.484 or 94.7% of its theoretical value of 0.511 when ethanol entrapped in the exhaust gas was incorporated. Meanwhile, quasi-steady states and non-steady oscillations were observed for residual glucose, ethanol and biomass concentrations for all of these bioreactors during their operations. Models that can be used to predict yeast cell lysis and viability loss were developed. SN - 0168-1656 UR - https://www.unboundmedicine.com/medline/citation/15163519/Continuous_ethanol_production_and_evaluation_of_yeast_cell_lysis_and_viability_loss_under_very_high_gravity_medium_conditions_ DB - PRIME DP - Unbound Medicine ER -