On-column labeling reaction for analysis of protein contents of a single cell using capillary electrophoresis with laser-induced fluorescence detection.
Methods Mol Biol. 2004; 276:29-38.MM

Abstract

This chapter presents methods for capillary electrophoresis (CE) fingerprinting of proteins in a cell extract and in single cells. A custom-made CE instrument with laser-induced fluorescence (LIF) detection, used for the analyses, is described. Detailed procedures are given for: (1) on column labeling of proteins with a fluorogenic reagent, 5-furoyl quinoline-3-carboxaldehyde, (2) CE separation of labeled proteins, (3) preparation of a protein extract from cultured cells, and (4) manipulations associated with analyses of proteins in single cells. More than 20 relevant publications are cited in this chapter to assist the reader with adopting the presented methods.

Authors+Show Affiliations

Ahmadzadeh H

Department of Chemistry, University of Minnesota, Minneapolis, MN., USA.

Krylov S

No affiliation info available

MeSH

AnimalsCell ExtractsElectrophoresis, CapillaryFluorescenceFluorescent DyesHumansLasersProteins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15163852

Citation

Ahmadzadeh, Hossein, and Sergey Krylov. "On-column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-induced Fluorescence Detection." Methods in Molecular Biology (Clifton, N.J.), vol. 276, 2004, pp. 29-38.

Ahmadzadeh H, Krylov S. On-column labeling reaction for analysis of protein contents of a single cell using capillary electrophoresis with laser-induced fluorescence detection. Methods Mol Biol. 2004;276:29-38.

Ahmadzadeh, H., & Krylov, S. (2004). On-column labeling reaction for analysis of protein contents of a single cell using capillary electrophoresis with laser-induced fluorescence detection. Methods in Molecular Biology (Clifton, N.J.), 276, 29-38.

Ahmadzadeh H, Krylov S. On-column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-induced Fluorescence Detection. Methods Mol Biol. 2004;276:29-38. PubMed PMID: 15163852.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - On-column labeling reaction for analysis of protein contents of a single cell using capillary electrophoresis with laser-induced fluorescence detection. AU - Ahmadzadeh,Hossein, AU - Krylov,Sergey, PY - 2004/5/28/pubmed PY - 2004/9/3/medline PY - 2004/5/28/entrez SP - 29 EP - 38 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 276 N2 - This chapter presents methods for capillary electrophoresis (CE) fingerprinting of proteins in a cell extract and in single cells. A custom-made CE instrument with laser-induced fluorescence (LIF) detection, used for the analyses, is described. Detailed procedures are given for: (1) on column labeling of proteins with a fluorogenic reagent, 5-furoyl quinoline-3-carboxaldehyde, (2) CE separation of labeled proteins, (3) preparation of a protein extract from cultured cells, and (4) manipulations associated with analyses of proteins in single cells. More than 20 relevant publications are cited in this chapter to assist the reader with adopting the presented methods. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/15163852/On_column_labeling_reaction_for_analysis_of_protein_contents_of_a_single_cell_using_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -

Tags

On-column labeling reaction for analysis of protein contents of a single cell using capillary electrophoresis with laser-induced fluorescence detection.Methods Mol Biol. 2004; 276:29-38.MM