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Capillary electrophoresis-electrospray ionization mass spectrometry of amino acids, peptides, and proteins.
Methods Mol Biol. 2004; 276:253-90.MM

Abstract

Separation in capillary electrophoresis (CE) is based on the movement of charged compounds inside a background electrolyte under an applied potential. Because the mechanism of separation of CE differs from that of conventional high-performance liquid chromatography (HPLC), where separation is based on the analyte's hydrophobic properties, CE is often used as a complementary technique to HPLC. In addition, because CE is performed in narrow capillaries at atmospheric pressure, it is used as an alternative to HPLC, capable of handling small sample volumes while providing shorter analysis times with higher efficiency. For the analysis of amino acid, protein, and peptide mixtures in small volume samples such as in single cells, CE has rapidly evolved as a preferred separation technique. The combination of a high-efficiency separation technique, such as CE, with mass spectrometry (MS) detection provides a powerful system for the analysis of complex biological mixtures. In this chapter, a theoretical and practical approach to achieving high-performance CE-MS is discussed and the utility of CE-MS for the analysis of amino acids, peptides, and proteins is demonstrated.

Authors+Show Affiliations

Moini M
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas, USA.

MeSH

Amino Acid SequenceAmino AcidsChromatography, High Pressure LiquidElectrophoresis, CapillaryHydrophobic and Hydrophilic InteractionsMolecular Sequence DataPeptide MappingPeptidesProteinsSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15163862

Citation

Moini, Mehdi. "Capillary Electrophoresis-electrospray Ionization Mass Spectrometry of Amino Acids, Peptides, and Proteins." Methods in Molecular Biology (Clifton, N.J.), vol. 276, 2004, pp. 253-90.
Moini M. Capillary electrophoresis-electrospray ionization mass spectrometry of amino acids, peptides, and proteins. Methods Mol Biol. 2004;276:253-90.
Moini, M. (2004). Capillary electrophoresis-electrospray ionization mass spectrometry of amino acids, peptides, and proteins. Methods in Molecular Biology (Clifton, N.J.), 276, 253-90.
Moini M. Capillary Electrophoresis-electrospray Ionization Mass Spectrometry of Amino Acids, Peptides, and Proteins. Methods Mol Biol. 2004;276:253-90. PubMed PMID: 15163862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoresis-electrospray ionization mass spectrometry of amino acids, peptides, and proteins. A1 - Moini,Mehdi, PY - 2004/5/28/pubmed PY - 2004/9/3/medline PY - 2004/5/28/entrez SP - 253 EP - 90 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 276 N2 - Separation in capillary electrophoresis (CE) is based on the movement of charged compounds inside a background electrolyte under an applied potential. Because the mechanism of separation of CE differs from that of conventional high-performance liquid chromatography (HPLC), where separation is based on the analyte's hydrophobic properties, CE is often used as a complementary technique to HPLC. In addition, because CE is performed in narrow capillaries at atmospheric pressure, it is used as an alternative to HPLC, capable of handling small sample volumes while providing shorter analysis times with higher efficiency. For the analysis of amino acid, protein, and peptide mixtures in small volume samples such as in single cells, CE has rapidly evolved as a preferred separation technique. The combination of a high-efficiency separation technique, such as CE, with mass spectrometry (MS) detection provides a powerful system for the analysis of complex biological mixtures. In this chapter, a theoretical and practical approach to achieving high-performance CE-MS is discussed and the utility of CE-MS for the analysis of amino acids, peptides, and proteins is demonstrated. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/15163862/Capillary_electrophoresis_electrospray_ionization_mass_spectrometry_of_amino_acids_peptides_and_proteins_ DB - PRIME DP - Unbound Medicine ER -

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