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Characterization of low-molecular-weight glutenin genes in Aegilops tauschii.
Theor Appl Genet. 2004 Sep; 109(5):1028-40.TA

Abstract

This paper reports the characterization of the low-molecular-weight (LMW) glutenin gene family of Aegilops tauschii (syn. Triticum tauschii), the D-genome donor of hexaploid wheat. By analysis of bacterial artificial chromosome (BAC) clones positive for hybridization with an LMW glutenin probe, seven unique LMW glutenin genes were identified. These genes were sequenced, including their untranslated 3' and 5' flanking regions. The deduced amino acid sequences of the genes revealed four putative active genes and three pseudogenes. All these genes had a very high level of similarity to LMW glutenins characterized in hexaploid wheat. The predicted molecular weights of the mature proteins were between 32.2 kDa and 39.6 kDa, and the predicted isoelectric points of the proteins were between 7.53 and 8.06. All the deduced proteins were of the LMW-m type. The organization of the seven LMW glutenin genes appears to be interspersed over at least several hundred kilo base pairs, as indicated by the presence of only one gene or pseudogene per BAC clone. Southern blot analysis of genomic DNA of Ae. tauschii and the BAC clones containing the seven LMW glutenin genes indicated that the BAC clones contained all LMW glutenin-hybridizing bands present in the genome. Two-dimensional gel electrophoresis of an LMW glutenin extract from Ae. tauschii was conducted and showed the presence of at least 11 distinct proteins. Further analysis indicated that some of the observed proteins were modified gliadins. These results suggest that the actual number of typical LMW glutenins may in fact be much lower than previously thought, with a number of modified gliadins also being present in the polymeric fraction.

Authors+Show Affiliations

Johal J
Commonwealth Scientific and Industrial Research Organisation, Plant Industry, Canberra, ACT, Australia.
Gianibelli MC
No affiliation info available
Rahman S
No affiliation info available
Morell MK
No affiliation info available
Gale KR
No affiliation info available

MeSH

Amino Acid SequenceBase SequenceBlotting, SouthernChromosomes, Artificial, BacterialDNA PrimersElectrophoresis, Gel, Two-DimensionalGliadinGlutensMolecular Sequence DataMultigene FamilyPlant ProteinsPoaceaeRestriction MappingSequence AlignmentSequence Analysis, DNATriticum

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15164175

Citation

Johal, J, et al. "Characterization of Low-molecular-weight Glutenin Genes in Aegilops Tauschii." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 109, no. 5, 2004, pp. 1028-40.
Johal J, Gianibelli MC, Rahman S, et al. Characterization of low-molecular-weight glutenin genes in Aegilops tauschii. Theor Appl Genet. 2004;109(5):1028-40.
Johal, J., Gianibelli, M. C., Rahman, S., Morell, M. K., & Gale, K. R. (2004). Characterization of low-molecular-weight glutenin genes in Aegilops tauschii. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 109(5), 1028-40.
Johal J, et al. Characterization of Low-molecular-weight Glutenin Genes in Aegilops Tauschii. Theor Appl Genet. 2004;109(5):1028-40. PubMed PMID: 15164175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of low-molecular-weight glutenin genes in Aegilops tauschii. AU - Johal,J, AU - Gianibelli,M C, AU - Rahman,S, AU - Morell,M K, AU - Gale,K R, PY - 2004/5/28/pubmed PY - 2004/12/22/medline PY - 2004/5/28/entrez SP - 1028 EP - 40 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 109 IS - 5 N2 - This paper reports the characterization of the low-molecular-weight (LMW) glutenin gene family of Aegilops tauschii (syn. Triticum tauschii), the D-genome donor of hexaploid wheat. By analysis of bacterial artificial chromosome (BAC) clones positive for hybridization with an LMW glutenin probe, seven unique LMW glutenin genes were identified. These genes were sequenced, including their untranslated 3' and 5' flanking regions. The deduced amino acid sequences of the genes revealed four putative active genes and three pseudogenes. All these genes had a very high level of similarity to LMW glutenins characterized in hexaploid wheat. The predicted molecular weights of the mature proteins were between 32.2 kDa and 39.6 kDa, and the predicted isoelectric points of the proteins were between 7.53 and 8.06. All the deduced proteins were of the LMW-m type. The organization of the seven LMW glutenin genes appears to be interspersed over at least several hundred kilo base pairs, as indicated by the presence of only one gene or pseudogene per BAC clone. Southern blot analysis of genomic DNA of Ae. tauschii and the BAC clones containing the seven LMW glutenin genes indicated that the BAC clones contained all LMW glutenin-hybridizing bands present in the genome. Two-dimensional gel electrophoresis of an LMW glutenin extract from Ae. tauschii was conducted and showed the presence of at least 11 distinct proteins. Further analysis indicated that some of the observed proteins were modified gliadins. These results suggest that the actual number of typical LMW glutenins may in fact be much lower than previously thought, with a number of modified gliadins also being present in the polymeric fraction. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/15164175/Characterization_of_low_molecular_weight_glutenin_genes_in_Aegilops_tauschii_ DB - PRIME DP - Unbound Medicine ER -