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Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins.
Mol Microbiol. 2004 Jun; 52(5):1413-25.MM

Abstract

The covalently linked cell wall protein Ccw12p of Saccharomyces cerevisiae is a GPI-anchored protein (V. Mrsa et al., 1999, J Bacteriol 181: 3076-3086). Although only 121 amino acids long, the haemagglutinin-tagged protein released by laminarinase from the cell wall possesses an apparent molecular mass of > 300 kDa. A membrane-bound form with an apparent molecular mass of 58 kDa is highly O- and N-glycosylated and contains the GPI anchor. With a half-life of 2 min, the membrane form is transformed to the > 300 kDa form. The deletion mutant ccw12Delta grows slower than the wild type, is highly sensitive to Calcofluor white and contains 2.5 times more chitin. Further, compared with wild-type yeast, significantly more proteins are released from intact cells when treated with dithiothreitol. Interestingly, these defects become less pronounced when further GPI-anchored cell wall proteins are deleted. Mutant DeltaGPI (simultaneous deletion of CCW12, CCW13/DAN1, CCW14, TIP1 and CWP1) is similar in many respects to wild-type yeast. To find out how the cell wall is stabilized in mutant DeltaGPI, a genome-wide transcription analysis was performed. Of 159 significantly regulated genes, 14 encode either known or suspected cell wall-associated proteins. Analysis of genes affected in transcription revealed that SED1 and SRL1 in particular are required to reconstruct cell wall stability in the absence of multiple GPI-anchored mannoproteins.

Authors+Show Affiliations

Hagen I
Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany.
Ecker M
No affiliation info available
Lagorce A
No affiliation info available
Francois JM
No affiliation info available
Sestak S
No affiliation info available
Rachel R
No affiliation info available
Grossmann G
No affiliation info available
Hauser NC
No affiliation info available
Hoheisel JD
No affiliation info available
Tanner W
No affiliation info available
Strahl S
No affiliation info available

MeSH

Cell WallChitinGene Expression ProfilingGene Expression Regulation, FungalGlycosylphosphatidylinositolsMembrane GlycoproteinsMutationOligonucleotide Array Sequence AnalysisPhenotypeProtein Processing, Post-TranslationalSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15165243

Citation

Hagen, Ilja, et al. "Sed1p and Srl1p Are Required to Compensate for Cell Wall Instability in Saccharomyces Cerevisiae Mutants Defective in Multiple GPI-anchored Mannoproteins." Molecular Microbiology, vol. 52, no. 5, 2004, pp. 1413-25.
Hagen I, Ecker M, Lagorce A, et al. Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins. Mol Microbiol. 2004;52(5):1413-25.
Hagen, I., Ecker, M., Lagorce, A., Francois, J. M., Sestak, S., Rachel, R., Grossmann, G., Hauser, N. C., Hoheisel, J. D., Tanner, W., & Strahl, S. (2004). Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins. Molecular Microbiology, 52(5), 1413-25.
Hagen I, et al. Sed1p and Srl1p Are Required to Compensate for Cell Wall Instability in Saccharomyces Cerevisiae Mutants Defective in Multiple GPI-anchored Mannoproteins. Mol Microbiol. 2004;52(5):1413-25. PubMed PMID: 15165243.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins. AU - Hagen,Ilja, AU - Ecker,Margit, AU - Lagorce,Arnaud, AU - Francois,Jean M, AU - Sestak,Sergej, AU - Rachel,Reinhard, AU - Grossmann,Guido, AU - Hauser,Nicole C, AU - Hoheisel,Jörg D, AU - Tanner,Widmar, AU - Strahl,Sabine, PY - 2004/5/29/pubmed PY - 2004/8/7/medline PY - 2004/5/29/entrez SP - 1413 EP - 25 JF - Molecular microbiology JO - Mol Microbiol VL - 52 IS - 5 N2 - The covalently linked cell wall protein Ccw12p of Saccharomyces cerevisiae is a GPI-anchored protein (V. Mrsa et al., 1999, J Bacteriol 181: 3076-3086). Although only 121 amino acids long, the haemagglutinin-tagged protein released by laminarinase from the cell wall possesses an apparent molecular mass of > 300 kDa. A membrane-bound form with an apparent molecular mass of 58 kDa is highly O- and N-glycosylated and contains the GPI anchor. With a half-life of 2 min, the membrane form is transformed to the > 300 kDa form. The deletion mutant ccw12Delta grows slower than the wild type, is highly sensitive to Calcofluor white and contains 2.5 times more chitin. Further, compared with wild-type yeast, significantly more proteins are released from intact cells when treated with dithiothreitol. Interestingly, these defects become less pronounced when further GPI-anchored cell wall proteins are deleted. Mutant DeltaGPI (simultaneous deletion of CCW12, CCW13/DAN1, CCW14, TIP1 and CWP1) is similar in many respects to wild-type yeast. To find out how the cell wall is stabilized in mutant DeltaGPI, a genome-wide transcription analysis was performed. Of 159 significantly regulated genes, 14 encode either known or suspected cell wall-associated proteins. Analysis of genes affected in transcription revealed that SED1 and SRL1 in particular are required to reconstruct cell wall stability in the absence of multiple GPI-anchored mannoproteins. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/15165243/Sed1p_and_Srl1p_are_required_to_compensate_for_cell_wall_instability_in_Saccharomyces_cerevisiae_mutants_defective_in_multiple_GPI_anchored_mannoproteins_ DB - PRIME DP - Unbound Medicine ER -