Tags

Type your tag names separated by a space and hit enter

Application of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis).
Aquat Toxicol. 2004 Mar 10; 66(4):381-92.AT

Abstract

Green-lipped mussels (Perna viridis) were exposed to water-borne benzo[a]pyrene (B[a]P) at nominal concentrations of 0, 0.3, 3 and 30 microg l(-1) for up to 12 days, and both the relative levels of DNA strand breaks (assessed using an alkaline comet assay) and the proportion of micronucleus (MN) formation were monitored in mussel haemocytes at days 0, 1, 3, 6 and 12. The results of the comet assay indicated that an increase in the proportion of strand breaks occurred generally with increasing B[a]P concentration, but a significant decrease in the levels of DNA damage was observed after exposure for 12 days at all concentrations tested, suggesting that the patterns of changes in the levels of DNA strand breakage can be explained by the threshold dependent DNA repair theory. Moreover, the relatively slow development and recovery of the DNA damage response in mussel haemocytes in comparison with previous findings utilizing P. viridis hepatopancreas suggests that the response of DNA alteration upon exposure to B[a]P may be tissue-specific in this species. Monitoring the frequency of micronucleus development in mussel haemocytes indicated both dose- and time-response relationships within the exposure period. Furthermore, the levels of DNA strand breakage correlated well with the levels of micronucleus induction, suggesting a possible cause and effect relationship between the two damage types. We suggest that DNA strand breakage and micronucleus formation in mussel haemocytes can potentially be used as convenient biomarkers of exposure to genotoxicants in the marine environment.

Authors+Show Affiliations

Department of Biology and Chemistry, Centre for Coastal Pollution and Conservation, City University of Hong Kong, Kowloon, Hong Kong SAR, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15168946

Citation

Siu, W H L., et al. "Application of the Comet and Micronucleus Assays to the Detection of B[a]P Genotoxicity in Haemocytes of the Green-lipped Mussel (Perna Viridis)." Aquatic Toxicology (Amsterdam, Netherlands), vol. 66, no. 4, 2004, pp. 381-92.
Siu WH, Cao J, Jack RW, et al. Application of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis). Aquat Toxicol. 2004;66(4):381-92.
Siu, W. H., Cao, J., Jack, R. W., Wu, R. S., Richardson, B. J., Xu, L., & Lam, P. K. (2004). Application of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis). Aquatic Toxicology (Amsterdam, Netherlands), 66(4), 381-92.
Siu WH, et al. Application of the Comet and Micronucleus Assays to the Detection of B[a]P Genotoxicity in Haemocytes of the Green-lipped Mussel (Perna Viridis). Aquat Toxicol. 2004 Mar 10;66(4):381-92. PubMed PMID: 15168946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of the comet and micronucleus assays to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis). AU - Siu,W H L, AU - Cao,J, AU - Jack,R W, AU - Wu,R S S, AU - Richardson,B J, AU - Xu,L, AU - Lam,P K S, PY - 2003/04/16/received PY - 2003/09/02/revised PY - 2003/10/24/accepted PY - 2004/6/1/pubmed PY - 2004/7/2/medline PY - 2004/6/1/entrez SP - 381 EP - 92 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 66 IS - 4 N2 - Green-lipped mussels (Perna viridis) were exposed to water-borne benzo[a]pyrene (B[a]P) at nominal concentrations of 0, 0.3, 3 and 30 microg l(-1) for up to 12 days, and both the relative levels of DNA strand breaks (assessed using an alkaline comet assay) and the proportion of micronucleus (MN) formation were monitored in mussel haemocytes at days 0, 1, 3, 6 and 12. The results of the comet assay indicated that an increase in the proportion of strand breaks occurred generally with increasing B[a]P concentration, but a significant decrease in the levels of DNA damage was observed after exposure for 12 days at all concentrations tested, suggesting that the patterns of changes in the levels of DNA strand breakage can be explained by the threshold dependent DNA repair theory. Moreover, the relatively slow development and recovery of the DNA damage response in mussel haemocytes in comparison with previous findings utilizing P. viridis hepatopancreas suggests that the response of DNA alteration upon exposure to B[a]P may be tissue-specific in this species. Monitoring the frequency of micronucleus development in mussel haemocytes indicated both dose- and time-response relationships within the exposure period. Furthermore, the levels of DNA strand breakage correlated well with the levels of micronucleus induction, suggesting a possible cause and effect relationship between the two damage types. We suggest that DNA strand breakage and micronucleus formation in mussel haemocytes can potentially be used as convenient biomarkers of exposure to genotoxicants in the marine environment. SN - 0166-445X UR - https://www.unboundmedicine.com/medline/citation/15168946/Application_of_the_comet_and_micronucleus_assays_to_the_detection_of_B[a]P_genotoxicity_in_haemocytes_of_the_green_lipped_mussel__Perna_viridis__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166445X03002200 DB - PRIME DP - Unbound Medicine ER -