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NMDA and AMPA receptors mediate intracellular calcium increase in rat cortical astrocytes.
Acta Pharmacol Sin. 2004 Jun; 25(6):714-20.AP

Abstract

AIM

To study the effect of glutamate on the intracellular calcium signal of pure cultured rat astrocytes and the role of N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors in the procedure.

METHODS

The fluorescence of calcium was measured by Fura-2/AM (F(345)/F(380)).

RESULTS

L-Glutamate induced [Ca(2+)](i) increase in most of the cells in concentration- and time-dependent manner. NMDA 50 mmol/L induced the fluorescence increase by almost three to four times, while the effect of AMPA 50 mmol/L was just half of that of D-(-)-2-amino-5-phosphonopentanoic acid (D-AP-5; a selective antagonist of the NMDA receptor). 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX, a selective antagonist of the AMPA receptor) abolished the effects of NMDA and AMPA, respectively. D-AP-5 and CNQX simultaneously or respectively attenuated the effect of L-glutamate at different degrees, but could not abolish it entirely.

CONCLUSION

Glutamate modulated intracellular Ca(2+) of pure cultured rat astrocytes through different pathways. The activation of NMDA and AMPA receptors took part in the complex mechanisms.

Authors+Show Affiliations

Neurology Department, Union Hospital, Tongji College, Huazhong University of Science and Technology, Wuhan 430022, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15169621

Citation

Hu, Bo, et al. "NMDA and AMPA Receptors Mediate Intracellular Calcium Increase in Rat Cortical Astrocytes." Acta Pharmacologica Sinica, vol. 25, no. 6, 2004, pp. 714-20.
Hu B, Sun SG, Tong ET. NMDA and AMPA receptors mediate intracellular calcium increase in rat cortical astrocytes. Acta Pharmacol Sin. 2004;25(6):714-20.
Hu, B., Sun, S. G., & Tong, E. T. (2004). NMDA and AMPA receptors mediate intracellular calcium increase in rat cortical astrocytes. Acta Pharmacologica Sinica, 25(6), 714-20.
Hu B, Sun SG, Tong ET. NMDA and AMPA Receptors Mediate Intracellular Calcium Increase in Rat Cortical Astrocytes. Acta Pharmacol Sin. 2004;25(6):714-20. PubMed PMID: 15169621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NMDA and AMPA receptors mediate intracellular calcium increase in rat cortical astrocytes. AU - Hu,Bo, AU - Sun,Sheng-Gang, AU - Tong,E-Tang, PY - 2004/6/1/pubmed PY - 2004/8/11/medline PY - 2004/6/1/entrez SP - 714 EP - 20 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 25 IS - 6 N2 - AIM: To study the effect of glutamate on the intracellular calcium signal of pure cultured rat astrocytes and the role of N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors in the procedure. METHODS: The fluorescence of calcium was measured by Fura-2/AM (F(345)/F(380)). RESULTS: L-Glutamate induced [Ca(2+)](i) increase in most of the cells in concentration- and time-dependent manner. NMDA 50 mmol/L induced the fluorescence increase by almost three to four times, while the effect of AMPA 50 mmol/L was just half of that of D-(-)-2-amino-5-phosphonopentanoic acid (D-AP-5; a selective antagonist of the NMDA receptor). 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX, a selective antagonist of the AMPA receptor) abolished the effects of NMDA and AMPA, respectively. D-AP-5 and CNQX simultaneously or respectively attenuated the effect of L-glutamate at different degrees, but could not abolish it entirely. CONCLUSION: Glutamate modulated intracellular Ca(2+) of pure cultured rat astrocytes through different pathways. The activation of NMDA and AMPA receptors took part in the complex mechanisms. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/15169621/NMDA_and_AMPA_receptors_mediate_intracellular_calcium_increase_in_rat_cortical_astrocytes_ L2 - http://www.chinaphar.com/1671-4083/25/714.pdf DB - PRIME DP - Unbound Medicine ER -