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Screening postmortem whole blood for oxycodone by ELISA response ratios.
J Forensic Sci 2004; 49(3):621-6JF

Abstract

The objective of this study was to investigate the accuracy of screening postmortem whole blood for oxycodone using the ratio of the oxycodone immunoassay response to the response for the specimen obtained with a general opiate-class immunoassay. Fifty eight specimens which were negative for opiates and 158 postmortem whole blood specimens positive for opiates including 66 specimens known to contain oxycodone were assayed. Specimens were diluted 1:5 with assay buffer and analyzed by both the Neogen Oxymorphone/Oxycodone ELISA and the Neogen Opiate Group ELISA (Neogen Corporation, Lexington KY). The oxycodone equivalents in ng/mL from the Oxymorphone/Oxycodone ELISA were divided by the morphine equivalents in ng/mL from the Opiates ELISA to obtain an Oxycodone/Opiates Response Ratio. This ratio was compared with the GC/MS data for all specimens and for opiate positive specimens. Receiver Operating Characteristic (ROC) analysis suggested that optimum relative response ratio was 2.0. The sensitivity of the ELISA response ratio for the presence of oxycodone at a response ratio cutoff of 2.0 was 89.4% +/- 3.8% and the specificity was 88.1% +/- 3.2%. Specimens with a ratio of 2.0 or higher had a greater than 50% probability (positive predictive value) of containing oxycodone in a population with a greater than 15% prevalence of oxycodone.

Authors+Show Affiliations

Spiehler and Associates, Newport Beach, CA, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Validation Studies

Language

eng

PubMed ID

15171187

Citation

Spiehler, Vina R., et al. "Screening Postmortem Whole Blood for Oxycodone By ELISA Response Ratios." Journal of Forensic Sciences, vol. 49, no. 3, 2004, pp. 621-6.
Spiehler VR, DeCicco L, McCutcheon JR, et al. Screening postmortem whole blood for oxycodone by ELISA response ratios. J Forensic Sci. 2004;49(3):621-6.
Spiehler, V. R., DeCicco, L., McCutcheon, J. R., Kupiec, T., & Kemp, P. (2004). Screening postmortem whole blood for oxycodone by ELISA response ratios. Journal of Forensic Sciences, 49(3), pp. 621-6.
Spiehler VR, et al. Screening Postmortem Whole Blood for Oxycodone By ELISA Response Ratios. J Forensic Sci. 2004;49(3):621-6. PubMed PMID: 15171187.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening postmortem whole blood for oxycodone by ELISA response ratios. AU - Spiehler,Vina R, AU - DeCicco,Lacinda, AU - McCutcheon,J Rod, AU - Kupiec,Tom, AU - Kemp,Philip, PY - 2004/6/3/pubmed PY - 2004/7/23/medline PY - 2004/6/3/entrez SP - 621 EP - 6 JF - Journal of forensic sciences JO - J. Forensic Sci. VL - 49 IS - 3 N2 - The objective of this study was to investigate the accuracy of screening postmortem whole blood for oxycodone using the ratio of the oxycodone immunoassay response to the response for the specimen obtained with a general opiate-class immunoassay. Fifty eight specimens which were negative for opiates and 158 postmortem whole blood specimens positive for opiates including 66 specimens known to contain oxycodone were assayed. Specimens were diluted 1:5 with assay buffer and analyzed by both the Neogen Oxymorphone/Oxycodone ELISA and the Neogen Opiate Group ELISA (Neogen Corporation, Lexington KY). The oxycodone equivalents in ng/mL from the Oxymorphone/Oxycodone ELISA were divided by the morphine equivalents in ng/mL from the Opiates ELISA to obtain an Oxycodone/Opiates Response Ratio. This ratio was compared with the GC/MS data for all specimens and for opiate positive specimens. Receiver Operating Characteristic (ROC) analysis suggested that optimum relative response ratio was 2.0. The sensitivity of the ELISA response ratio for the presence of oxycodone at a response ratio cutoff of 2.0 was 89.4% +/- 3.8% and the specificity was 88.1% +/- 3.2%. Specimens with a ratio of 2.0 or higher had a greater than 50% probability (positive predictive value) of containing oxycodone in a population with a greater than 15% prevalence of oxycodone. SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/15171187/Screening_postmortem_whole_blood_for_oxycodone_by_ELISA_response_ratios_ DB - PRIME DP - Unbound Medicine ER -