Tags

Type your tag names separated by a space and hit enter

Validated semiquantitative/quantitative screening of 51 drugs in whole blood as silylated derivatives by gas chromatography-selected ion monitoring mass spectrometry and gas chromatography electron capture detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jul 05; 806(2):205-19.JC

Abstract

A comprehensively validated procedure is presented for simultaneous semiquantitative/quantitative screening of 51 drugs of abuse or drugs potentially hazardous for traffic safety in serum, plasma or whole blood. Benzodiazepines (12), cannabinoids (3), opioids (8), cocaine, antidepressants (13), antipsychotics (5) and antiepileptics (2) as well as zolpidem, zaleplon, zopiclone, meprobamate, carisoprodol, tizanidine and orphenadrine and internal standard flurazepam, were isolated by high-yield liquid-liquid extraction (LLE). The dried extracts were derivatized by two-step silylation and analyzed by the combination of two different gas chromatographic (GC) separations with both electron capture detection (ECD) and mass spectrometry (MS) operating in a selected ion-monitoring (SIM) mode. Quantitative or semiquantitative results were obtained for each substance based on four-point calibration. In the validation tests, accuracy, reproducibility, linearity, limit of detection (LOD) and limit of quantitation (LOQ), selectivity, as well as extraction efficiency and stability of standard stock solutions were tested, and derivatization was optimized in detail. Intra- and inter-day precisions were within 2.5-21.8 and 6.0-22.5%, and square of correlation coefficients of linearity ranged from 0.9896 to 0.9999. The limit of quantitation (LOQ) varied from 2 to 2000 ng/ml due to a variety of the relevant concentrations of the analyzed substances in blood. The method is feasible for highly sensitive, reliable and possibly routinely performed clinical and forensic toxicological analyses.

Authors+Show Affiliations

Laboratory of Substance Abuse, National Public Health Institute, Mannerheimintie 166, Helsinki 00300, Finland. teemu.gunnar@ktl.fiNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

15171931

Citation

Gunnar, Teemu, et al. "Validated Semiquantitative/quantitative Screening of 51 Drugs in Whole Blood as Silylated Derivatives By Gas Chromatography-selected Ion Monitoring Mass Spectrometry and Gas Chromatography Electron Capture Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 806, no. 2, 2004, pp. 205-19.
Gunnar T, Mykkänen S, Ariniemi K, et al. Validated semiquantitative/quantitative screening of 51 drugs in whole blood as silylated derivatives by gas chromatography-selected ion monitoring mass spectrometry and gas chromatography electron capture detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;806(2):205-19.
Gunnar, T., Mykkänen, S., Ariniemi, K., & Lillsunde, P. (2004). Validated semiquantitative/quantitative screening of 51 drugs in whole blood as silylated derivatives by gas chromatography-selected ion monitoring mass spectrometry and gas chromatography electron capture detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 806(2), 205-19.
Gunnar T, et al. Validated Semiquantitative/quantitative Screening of 51 Drugs in Whole Blood as Silylated Derivatives By Gas Chromatography-selected Ion Monitoring Mass Spectrometry and Gas Chromatography Electron Capture Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jul 5;806(2):205-19. PubMed PMID: 15171931.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validated semiquantitative/quantitative screening of 51 drugs in whole blood as silylated derivatives by gas chromatography-selected ion monitoring mass spectrometry and gas chromatography electron capture detection. AU - Gunnar,Teemu, AU - Mykkänen,Sirpa, AU - Ariniemi,Kari, AU - Lillsunde,Pirjo, PY - 2003/09/03/received PY - 2004/03/31/revised PY - 2004/03/31/accepted PY - 2004/6/3/pubmed PY - 2005/2/25/medline PY - 2004/6/3/entrez SP - 205 EP - 19 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 806 IS - 2 N2 - A comprehensively validated procedure is presented for simultaneous semiquantitative/quantitative screening of 51 drugs of abuse or drugs potentially hazardous for traffic safety in serum, plasma or whole blood. Benzodiazepines (12), cannabinoids (3), opioids (8), cocaine, antidepressants (13), antipsychotics (5) and antiepileptics (2) as well as zolpidem, zaleplon, zopiclone, meprobamate, carisoprodol, tizanidine and orphenadrine and internal standard flurazepam, were isolated by high-yield liquid-liquid extraction (LLE). The dried extracts were derivatized by two-step silylation and analyzed by the combination of two different gas chromatographic (GC) separations with both electron capture detection (ECD) and mass spectrometry (MS) operating in a selected ion-monitoring (SIM) mode. Quantitative or semiquantitative results were obtained for each substance based on four-point calibration. In the validation tests, accuracy, reproducibility, linearity, limit of detection (LOD) and limit of quantitation (LOQ), selectivity, as well as extraction efficiency and stability of standard stock solutions were tested, and derivatization was optimized in detail. Intra- and inter-day precisions were within 2.5-21.8 and 6.0-22.5%, and square of correlation coefficients of linearity ranged from 0.9896 to 0.9999. The limit of quantitation (LOQ) varied from 2 to 2000 ng/ml due to a variety of the relevant concentrations of the analyzed substances in blood. The method is feasible for highly sensitive, reliable and possibly routinely performed clinical and forensic toxicological analyses. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15171931/Validated_semiquantitative/quantitative_screening_of_51_drugs_in_whole_blood_as_silylated_derivatives_by_gas_chromatography_selected_ion_monitoring_mass_spectrometry_and_gas_chromatography_electron_capture_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570023204003241 DB - PRIME DP - Unbound Medicine ER -