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Simultaneous determination of vigabatrin and amino acid neurotransmitters in brain microdialysates by capillary electrophoresis with laser-induced fluorescence detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jul 05; 806(2):237-44.JC

Abstract

Capillary electrophoresis with laser-induced fluorescence detection (CE-LIFD) coupled to in vivo microdialysis sampling was used in order to monitor simultaneously a drug and several neurotransmitters in the brain extracellular fluid. Determination of the antiepileptic drug vigabatrin and the amino acid neurotransmitters glutamate (Glu), l-aspartate (l-Asp) and gamma-aminobutyric acid (GABA) was performed on low-concentration samples which were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) and separated using a pH 9.2 75 mM sodium borate running buffer containing 60 mM sodium dodecyl sulfate (SDS) and 5mM hydroxypropyl-beta-cyclodextrin (HP-beta-CD). Glu, l-Asp and vigabatrin derivatized at a concentration of 1.0 x 10(-9) M, and GABA derivatized at a concentration of 5.0 x 10(-9) M, produced peaks with signal-to-noise ratios of 8:1, 8:1, 4:1 and 5:1, respectively. The nature of the neurotransmitter peaks found in rat brain microdialysates was confirmed by both electrophoretic and pharmacological validations. This method was used for monitoring vigabatrin and amino acid neurotransmitters in microdialysates from the rat striatum during intracerebral infusion of the drug and revealed rapid vigabatrin-induced changes in GABA and Glu levels. This original application of CE-LIFD coupled to microdialysis represents a powerful tool for pharmacokinetic/pharmacodynamic investigations.

Authors+Show Affiliations

Laboratoire de Neuropharmacologie et Neurochimie, INSERM U512, Faculté de Pharmacie, et Institut Fédératif des Neurosciences de Lyon, IFR 19, Université Claude Bernard, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15171934

Citation

Benturquia, Nadia, et al. "Simultaneous Determination of Vigabatrin and Amino Acid Neurotransmitters in Brain Microdialysates By Capillary Electrophoresis With Laser-induced Fluorescence Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 806, no. 2, 2004, pp. 237-44.
Benturquia N, Parrot S, Sauvinet V, et al. Simultaneous determination of vigabatrin and amino acid neurotransmitters in brain microdialysates by capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;806(2):237-44.
Benturquia, N., Parrot, S., Sauvinet, V., Renaud, B., & Denoroy, L. (2004). Simultaneous determination of vigabatrin and amino acid neurotransmitters in brain microdialysates by capillary electrophoresis with laser-induced fluorescence detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 806(2), 237-44.
Benturquia N, et al. Simultaneous Determination of Vigabatrin and Amino Acid Neurotransmitters in Brain Microdialysates By Capillary Electrophoresis With Laser-induced Fluorescence Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jul 5;806(2):237-44. PubMed PMID: 15171934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of vigabatrin and amino acid neurotransmitters in brain microdialysates by capillary electrophoresis with laser-induced fluorescence detection. AU - Benturquia,Nadia, AU - Parrot,Sandrine, AU - Sauvinet,Valérie, AU - Renaud,Bernard, AU - Denoroy,Luc, PY - 2004/01/12/received PY - 2004/03/31/revised PY - 2004/03/31/accepted PY - 2004/6/3/pubmed PY - 2005/2/25/medline PY - 2004/6/3/entrez SP - 237 EP - 44 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 806 IS - 2 N2 - Capillary electrophoresis with laser-induced fluorescence detection (CE-LIFD) coupled to in vivo microdialysis sampling was used in order to monitor simultaneously a drug and several neurotransmitters in the brain extracellular fluid. Determination of the antiepileptic drug vigabatrin and the amino acid neurotransmitters glutamate (Glu), l-aspartate (l-Asp) and gamma-aminobutyric acid (GABA) was performed on low-concentration samples which were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) and separated using a pH 9.2 75 mM sodium borate running buffer containing 60 mM sodium dodecyl sulfate (SDS) and 5mM hydroxypropyl-beta-cyclodextrin (HP-beta-CD). Glu, l-Asp and vigabatrin derivatized at a concentration of 1.0 x 10(-9) M, and GABA derivatized at a concentration of 5.0 x 10(-9) M, produced peaks with signal-to-noise ratios of 8:1, 8:1, 4:1 and 5:1, respectively. The nature of the neurotransmitter peaks found in rat brain microdialysates was confirmed by both electrophoretic and pharmacological validations. This method was used for monitoring vigabatrin and amino acid neurotransmitters in microdialysates from the rat striatum during intracerebral infusion of the drug and revealed rapid vigabatrin-induced changes in GABA and Glu levels. This original application of CE-LIFD coupled to microdialysis represents a powerful tool for pharmacokinetic/pharmacodynamic investigations. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15171934/Simultaneous_determination_of_vigabatrin_and_amino_acid_neurotransmitters_in_brain_microdialysates_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570023204003137 DB - PRIME DP - Unbound Medicine ER -