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IgA and IgG tissue transglutaminase antibodies in systemic lupus erythematosus.
Lupus 2004; 13(4):241-4L

Abstract

Systemic lupus erythematosus (SLE) and coeliac disease (CD) are diseases of an autoimmune origin that share the human leukocyte HLA-B8 and HLA-DR3 histocompatibility antigens, yet the co-association of CD with SLE is mainly based on case reports. Thus, the real prevalence of CD in SLE is unclear. The aim of this study was to determine the prevalence of antitissue transglutaminase (anti-tTG) in SLE and the relation between SLE and CD. In this case-control study, 100 patients with SLE, and 120 healthy subjects were studied. Sera from all participants were analysed for the presence of IgA and IgG anti-tTG antibodies using a human recombinant tissue transglutaminase (tTG) immuno-enzymatic assay. Anti-tTG positive patients and controls were further tested for antiendomysial (EMA) antibodies by an indirect immunofluorescence and HLA typing (DQalpha1*0501-DQbeta1*0201 allele determination). Subjects who had EMA or the mentioned allele, underwent duodenal biopsy to confirm a possible diagnosis of CD. Anti-tTG antibodies (IgA or IgG isotypes) were found in three of the 100 SLE patients (overall prevalence of 3%): one had the IgA and two the IgG isotypes. Only 1 of 120 healthy subjects (0.8%) had a low positive reaction for IgA anti-tTG. Only the IgA anti-tTG positive SLE patient was diagnosed as having CD based on a positive IgA-EMA and small bowel biopsy findings. The two IgG anti-tTG positive SLE patients and the IgA anti-tTG positive healthy subject were classified as false positives (EMA negative and HLA DQalpha1*0501-DQbeta1*0201 allele negative). In conclusion, anti-tTG antibodies were found at a low rate in SLE patients and mostly did not indicate the presence of CD. Thus, serological screening for CD is not recommended in SLE, unless a clinical suspicion of CD is present.

Authors+Show Affiliations

Department of Medicine B and Center for Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer, Israel.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15176659

Citation

Marai, I, et al. "IgA and IgG Tissue Transglutaminase Antibodies in Systemic Lupus Erythematosus." Lupus, vol. 13, no. 4, 2004, pp. 241-4.
Marai I, Shoenfeld Y, Bizzaro N, et al. IgA and IgG tissue transglutaminase antibodies in systemic lupus erythematosus. Lupus. 2004;13(4):241-4.
Marai, I., Shoenfeld, Y., Bizzaro, N., Villalta, D., Doria, A., Tonutti, E., & Tozzoli, R. (2004). IgA and IgG tissue transglutaminase antibodies in systemic lupus erythematosus. Lupus, 13(4), pp. 241-4.
Marai I, et al. IgA and IgG Tissue Transglutaminase Antibodies in Systemic Lupus Erythematosus. Lupus. 2004;13(4):241-4. PubMed PMID: 15176659.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IgA and IgG tissue transglutaminase antibodies in systemic lupus erythematosus. AU - Marai,I, AU - Shoenfeld,Y, AU - Bizzaro,N, AU - Villalta,D, AU - Doria,A, AU - Tonutti,E, AU - Tozzoli,R, PY - 2004/6/5/pubmed PY - 2004/8/20/medline PY - 2004/6/5/entrez SP - 241 EP - 4 JF - Lupus JO - Lupus VL - 13 IS - 4 N2 - Systemic lupus erythematosus (SLE) and coeliac disease (CD) are diseases of an autoimmune origin that share the human leukocyte HLA-B8 and HLA-DR3 histocompatibility antigens, yet the co-association of CD with SLE is mainly based on case reports. Thus, the real prevalence of CD in SLE is unclear. The aim of this study was to determine the prevalence of antitissue transglutaminase (anti-tTG) in SLE and the relation between SLE and CD. In this case-control study, 100 patients with SLE, and 120 healthy subjects were studied. Sera from all participants were analysed for the presence of IgA and IgG anti-tTG antibodies using a human recombinant tissue transglutaminase (tTG) immuno-enzymatic assay. Anti-tTG positive patients and controls were further tested for antiendomysial (EMA) antibodies by an indirect immunofluorescence and HLA typing (DQalpha1*0501-DQbeta1*0201 allele determination). Subjects who had EMA or the mentioned allele, underwent duodenal biopsy to confirm a possible diagnosis of CD. Anti-tTG antibodies (IgA or IgG isotypes) were found in three of the 100 SLE patients (overall prevalence of 3%): one had the IgA and two the IgG isotypes. Only 1 of 120 healthy subjects (0.8%) had a low positive reaction for IgA anti-tTG. Only the IgA anti-tTG positive SLE patient was diagnosed as having CD based on a positive IgA-EMA and small bowel biopsy findings. The two IgG anti-tTG positive SLE patients and the IgA anti-tTG positive healthy subject were classified as false positives (EMA negative and HLA DQalpha1*0501-DQbeta1*0201 allele negative). In conclusion, anti-tTG antibodies were found at a low rate in SLE patients and mostly did not indicate the presence of CD. Thus, serological screening for CD is not recommended in SLE, unless a clinical suspicion of CD is present. SN - 0961-2033 UR - https://www.unboundmedicine.com/medline/citation/15176659/IgA_and_IgG_tissue_transglutaminase_antibodies_in_systemic_lupus_erythematosus_ L2 - http://journals.sagepub.com/doi/full/10.1191/0961203304lu1004oa?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -