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Effects of high-fat feeding and fasting on ghrelin expression in the mouse stomach.
Regul Pept. 2004 Aug 15; 120(1-3):261-7.RP

Abstract

Ghrelin is a peptide identified as an endogenous ligand for the growth hormone secretagogue receptor. Studies have shown that ghrelin stimulates growth hormone, promotes food intake and decreases energy expenditure. Furthermore, feeding status seems to influence plasma ghrelin levels, as these are increased during fasting, whereas feeding and oral glucose intake reduce plasma ghrelin. This study examined whether standardized obesity and fasting affect cellular expression of ghrelin. Specimens from the gastrointestinal tract of fed or 18-h fasted, low-fat or high-fat fed (10 weeks on diet) C57BL/6J mice were studied by immunocytochemistry (ICC) for ghrelin and in situ hybridization (ISH) for ghrelin mRNA. Ghrelin was expressed in especially the corpus but also the antrum of the stomach of all groups studied. Cells positive for ghrelin and ghrelin mRNA in the stomach were reduced in high-fat fed mice. In contrast, ghrelin expression was not affected by fasting. The reduction in ghrelin expression in the high-fat fed mice was associated with a reduction in plasma levels of ghrelin, whereas after fasting, when expression rate was not altered, there was an increase in plasma ghrelin. In conclusion, ghrelin is highly expressed in the corpus and antrum of the stomach of C57BL/6J mice. This expression is reduced in obesity, whereas fasting has no effect.

Authors+Show Affiliations

Department of Medicine, Lund University, Sweden. som@kvl.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15177945

Citation

Moesgaard, Sophia G., et al. "Effects of High-fat Feeding and Fasting On Ghrelin Expression in the Mouse Stomach." Regulatory Peptides, vol. 120, no. 1-3, 2004, pp. 261-7.
Moesgaard SG, Ahrén B, Carr RD, et al. Effects of high-fat feeding and fasting on ghrelin expression in the mouse stomach. Regul Pept. 2004;120(1-3):261-7.
Moesgaard, S. G., Ahrén, B., Carr, R. D., Gram, D. X., Brand, C. L., & Sundler, F. (2004). Effects of high-fat feeding and fasting on ghrelin expression in the mouse stomach. Regulatory Peptides, 120(1-3), 261-7.
Moesgaard SG, et al. Effects of High-fat Feeding and Fasting On Ghrelin Expression in the Mouse Stomach. Regul Pept. 2004 Aug 15;120(1-3):261-7. PubMed PMID: 15177945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of high-fat feeding and fasting on ghrelin expression in the mouse stomach. AU - Moesgaard,Sophia G, AU - Ahrén,Bo, AU - Carr,Richard D, AU - Gram,Dorte X, AU - Brand,Christian L, AU - Sundler,Frank, PY - 2003/10/24/received PY - 2004/03/18/revised PY - 2004/03/26/accepted PY - 2004/6/5/pubmed PY - 2005/1/12/medline PY - 2004/6/5/entrez SP - 261 EP - 7 JF - Regulatory peptides JO - Regul. Pept. VL - 120 IS - 1-3 N2 - Ghrelin is a peptide identified as an endogenous ligand for the growth hormone secretagogue receptor. Studies have shown that ghrelin stimulates growth hormone, promotes food intake and decreases energy expenditure. Furthermore, feeding status seems to influence plasma ghrelin levels, as these are increased during fasting, whereas feeding and oral glucose intake reduce plasma ghrelin. This study examined whether standardized obesity and fasting affect cellular expression of ghrelin. Specimens from the gastrointestinal tract of fed or 18-h fasted, low-fat or high-fat fed (10 weeks on diet) C57BL/6J mice were studied by immunocytochemistry (ICC) for ghrelin and in situ hybridization (ISH) for ghrelin mRNA. Ghrelin was expressed in especially the corpus but also the antrum of the stomach of all groups studied. Cells positive for ghrelin and ghrelin mRNA in the stomach were reduced in high-fat fed mice. In contrast, ghrelin expression was not affected by fasting. The reduction in ghrelin expression in the high-fat fed mice was associated with a reduction in plasma levels of ghrelin, whereas after fasting, when expression rate was not altered, there was an increase in plasma ghrelin. In conclusion, ghrelin is highly expressed in the corpus and antrum of the stomach of C57BL/6J mice. This expression is reduced in obesity, whereas fasting has no effect. SN - 0167-0115 UR - https://www.unboundmedicine.com/medline/citation/15177945/Effects_of_high_fat_feeding_and_fasting_on_ghrelin_expression_in_the_mouse_stomach_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167011504001004 DB - PRIME DP - Unbound Medicine ER -