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Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay.
Mar Environ Res. 2004 Aug-Dec; 58(2-5):701-5.ME

Abstract

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.

Authors+Show Affiliations

RF-Akvamiljø, Mekjarvik 12, 4070 Randaberg, Norway. ingrid.christina.taban@rf.noNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15178101

Citation

Taban, Ingrid Christina, et al. "Detection of DNA Damage in Mussels and Sea Urchins Exposed to Crude Oil Using Comet Assay." Marine Environmental Research, vol. 58, no. 2-5, 2004, pp. 701-5.
Taban IC, Bechmann RK, Torgrimsen S, et al. Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay. Mar Environ Res. 2004;58(2-5):701-5.
Taban, I. C., Bechmann, R. K., Torgrimsen, S., Baussant, T., & Sanni, S. (2004). Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay. Marine Environmental Research, 58(2-5), 701-5.
Taban IC, et al. Detection of DNA Damage in Mussels and Sea Urchins Exposed to Crude Oil Using Comet Assay. Mar Environ Res. 2004 Aug-Dec;58(2-5):701-5. PubMed PMID: 15178101.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay. AU - Taban,Ingrid Christina, AU - Bechmann,Renée Katrin, AU - Torgrimsen,Sigfryd, AU - Baussant,Thierry, AU - Sanni,Steinar, PY - 2004/6/5/pubmed PY - 2004/8/31/medline PY - 2004/6/5/entrez SP - 701 EP - 5 JF - Marine environmental research JO - Mar Environ Res VL - 58 IS - 2-5 N2 - The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination. SN - 0141-1136 UR - https://www.unboundmedicine.com/medline/citation/15178101/Detection_of_DNA_damage_in_mussels_and_sea_urchins_exposed_to_crude_oil_using_comet_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-1136(04)00115-1 DB - PRIME DP - Unbound Medicine ER -