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Enterocyte metabolism during early adaptation after extensive intestinal resection in a rat model.
Surgery. 2004 Jun; 135(6):649-56.S

Abstract

OBJECTIVE

A better knowledge of intestinal adaptation after resection is required to improve the nutritional support that is given to patients. The aim of this study was to understand the metabolic changes underlying early adaptation after massive intestinal resection.

METHODS

Rats were assigned to either 80% intestinal resection or transection. All animals received the same intragastric nutrition. On day 8, plasma glutamine turnover was measured. Substrate use was determined on isolated enterocytes that were incubated in the presence of D-[U-(14)C] glucose (2 mmol/L), L-[U-(14)C] glutamine (2 mmol/L), L-[U-(14)C] arginine (1 mmol/L), or L-[1-(14)C] ornithine (1 mmol/L).

RESULTS

Plasma glutamine turnover was similar in both groups. The rate of enterocyte glutamine use was significantly increased in the resection group, although the maximal glutaminase activity was unchanged. Glutathione generation was enhanced 3-fold in remnant intestine as compared with transected intestine (P <.05). L-ornithine decarboxylation was increased markedly in resected animals (P <.05), without any detectable change of maximal ornithine decarboxylase activity.

CONCLUSION

The early phase of intestinal adaptation after resection induces changes in enterocyte glutamine and ornithine metabolism that may be related, in part, to increased de novo polyamine synthesis. This observation suggests that a supplementation of artificial nutrition by nutrients that lead to the generation of trophic agents may be of potential interest.

Authors+Show Affiliations

Laboratoire de Nutrition et Sécurité Alimentaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15179371

Citation

Lardy, Hubert, et al. "Enterocyte Metabolism During Early Adaptation After Extensive Intestinal Resection in a Rat Model." Surgery, vol. 135, no. 6, 2004, pp. 649-56.
Lardy H, Mouillé B, Thomas M, et al. Enterocyte metabolism during early adaptation after extensive intestinal resection in a rat model. Surgery. 2004;135(6):649-56.
Lardy, H., Mouillé, B., Thomas, M., Darcy-Vrillon, B., Vaugelade, P., Blachier, F., Bernard, F., Cherbuy, C., Robert, V., Corriol, O., Ricour, C., Goulet, O., Duée, P. H., & Colomb, V. (2004). Enterocyte metabolism during early adaptation after extensive intestinal resection in a rat model. Surgery, 135(6), 649-56.
Lardy H, et al. Enterocyte Metabolism During Early Adaptation After Extensive Intestinal Resection in a Rat Model. Surgery. 2004;135(6):649-56. PubMed PMID: 15179371.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enterocyte metabolism during early adaptation after extensive intestinal resection in a rat model. AU - Lardy,Hubert, AU - Mouillé,Béatrice, AU - Thomas,Muriel, AU - Darcy-Vrillon,Béatrice, AU - Vaugelade,Pierre, AU - Blachier,François, AU - Bernard,Françoise, AU - Cherbuy,Claire, AU - Robert,Véronique, AU - Corriol,Odile, AU - Ricour,Claude, AU - Goulet,Olivier, AU - Duée,Pierre-Henri, AU - Colomb,Virginie, PY - 2004/6/5/pubmed PY - 2004/7/2/medline PY - 2004/6/5/entrez SP - 649 EP - 56 JF - Surgery JO - Surgery VL - 135 IS - 6 N2 - OBJECTIVE: A better knowledge of intestinal adaptation after resection is required to improve the nutritional support that is given to patients. The aim of this study was to understand the metabolic changes underlying early adaptation after massive intestinal resection. METHODS: Rats were assigned to either 80% intestinal resection or transection. All animals received the same intragastric nutrition. On day 8, plasma glutamine turnover was measured. Substrate use was determined on isolated enterocytes that were incubated in the presence of D-[U-(14)C] glucose (2 mmol/L), L-[U-(14)C] glutamine (2 mmol/L), L-[U-(14)C] arginine (1 mmol/L), or L-[1-(14)C] ornithine (1 mmol/L). RESULTS: Plasma glutamine turnover was similar in both groups. The rate of enterocyte glutamine use was significantly increased in the resection group, although the maximal glutaminase activity was unchanged. Glutathione generation was enhanced 3-fold in remnant intestine as compared with transected intestine (P <.05). L-ornithine decarboxylation was increased markedly in resected animals (P <.05), without any detectable change of maximal ornithine decarboxylase activity. CONCLUSION: The early phase of intestinal adaptation after resection induces changes in enterocyte glutamine and ornithine metabolism that may be related, in part, to increased de novo polyamine synthesis. This observation suggests that a supplementation of artificial nutrition by nutrients that lead to the generation of trophic agents may be of potential interest. SN - 0039-6060 UR - https://www.unboundmedicine.com/medline/citation/15179371/Enterocyte_metabolism_during_early_adaptation_after_extensive_intestinal_resection_in_a_rat_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039606003007001 DB - PRIME DP - Unbound Medicine ER -