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Flavonol glycosides from the aerial parts of Aceriphyllum rossii and their antioxidant activities.
Arch Pharm Res. 2004 Apr; 27(4):390-5.AP

Abstract

The methanol extract obtained from the aerial parts of Aceriphyllum rossii (Saxifragaceae) was fractionated into ethyl acetate (EtOAc), n-BuOH and H2O layers through solvent fractionation. Repeated silica gel column chromatography of EtOAc and n-BuOH layers afforded six flavonol glycosides. They were identified as kaempferol 3-O-beta-D-glucopyranoside (astragalin, 1), quercetin 3-O-beta-D-glucopyranoside (isoquercitrin, 2), kaempferol 3-O-alpha-L-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside (3), quercetin 3-O-alpha-L-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside (rutin, 4), kaempferol 3-O-[alpha-L-rhamnopyranosyl (1-->4)-alpha-L-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside] (5) and quercetin 3-O-[alpha-L-rhamnopyranosyl (1-->4)-alpha-L-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside] (6) on the basis of several spectral data. The antioxidant activity of the six compounds was investigated using two free radicals such as the ABTS free radical and superoxide anion radical. Compound 1 exhibited the highest antioxidant activity in the ABTS [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] radical scavenging method. 100 mg/L of compound 1 was equivalent to 72.1+/-1.4 mg/L of vitamin C, and those of compounds 3 and 5 were equivalent to 62.7+/-0.5 mg/L and 54.3+/-1.3 mg/L of vitamin C, respectively. And in the superoxide anion radical scavenging method, compound 5 exhibited the highest activity with an IC50 value of 17.6+/-0.3 microM. In addition, some physical and spectral data of the flavonoids were confirmed.

Authors+Show Affiliations

Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, Suwon 449-701, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15180303

Citation

Han, Jae-Taek, et al. "Flavonol Glycosides From the Aerial Parts of Aceriphyllum Rossii and Their Antioxidant Activities." Archives of Pharmacal Research, vol. 27, no. 4, 2004, pp. 390-5.
Han JT, Bang MH, Chun OK, et al. Flavonol glycosides from the aerial parts of Aceriphyllum rossii and their antioxidant activities. Arch Pharm Res. 2004;27(4):390-5.
Han, J. T., Bang, M. H., Chun, O. K., Kim, D. O., Lee, C. Y., & Baek, N. I. (2004). Flavonol glycosides from the aerial parts of Aceriphyllum rossii and their antioxidant activities. Archives of Pharmacal Research, 27(4), 390-5.
Han JT, et al. Flavonol Glycosides From the Aerial Parts of Aceriphyllum Rossii and Their Antioxidant Activities. Arch Pharm Res. 2004;27(4):390-5. PubMed PMID: 15180303.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flavonol glycosides from the aerial parts of Aceriphyllum rossii and their antioxidant activities. AU - Han,Jae-Taek, AU - Bang,Myun-Ho, AU - Chun,Ock-Kyoung, AU - Kim,Dae-Ok, AU - Lee,Chang-Yong, AU - Baek,Nam-In, PY - 2004/6/8/pubmed PY - 2004/12/16/medline PY - 2004/6/8/entrez SP - 390 EP - 5 JF - Archives of pharmacal research JO - Arch Pharm Res VL - 27 IS - 4 N2 - The methanol extract obtained from the aerial parts of Aceriphyllum rossii (Saxifragaceae) was fractionated into ethyl acetate (EtOAc), n-BuOH and H2O layers through solvent fractionation. Repeated silica gel column chromatography of EtOAc and n-BuOH layers afforded six flavonol glycosides. They were identified as kaempferol 3-O-beta-D-glucopyranoside (astragalin, 1), quercetin 3-O-beta-D-glucopyranoside (isoquercitrin, 2), kaempferol 3-O-alpha-L-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside (3), quercetin 3-O-alpha-L-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside (rutin, 4), kaempferol 3-O-[alpha-L-rhamnopyranosyl (1-->4)-alpha-L-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside] (5) and quercetin 3-O-[alpha-L-rhamnopyranosyl (1-->4)-alpha-L-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside] (6) on the basis of several spectral data. The antioxidant activity of the six compounds was investigated using two free radicals such as the ABTS free radical and superoxide anion radical. Compound 1 exhibited the highest antioxidant activity in the ABTS [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] radical scavenging method. 100 mg/L of compound 1 was equivalent to 72.1+/-1.4 mg/L of vitamin C, and those of compounds 3 and 5 were equivalent to 62.7+/-0.5 mg/L and 54.3+/-1.3 mg/L of vitamin C, respectively. And in the superoxide anion radical scavenging method, compound 5 exhibited the highest activity with an IC50 value of 17.6+/-0.3 microM. In addition, some physical and spectral data of the flavonoids were confirmed. SN - 0253-6269 UR - https://www.unboundmedicine.com/medline/citation/15180303/Flavonol_glycosides_from_the_aerial_parts_of_Aceriphyllum_rossii_and_their_antioxidant_activities_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=15180303.ui DB - PRIME DP - Unbound Medicine ER -