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Isolation and phylogenetic analysis of novel viruses infecting the phytoplankton Phaeocystis globosa (Prymnesiophyceae).
Appl Environ Microbiol. 2004 Jun; 70(6):3700-5.AE

Abstract

Viruses infecting the harmful bloom-causing alga Phaeocystis globosa (Prymnesiophyceae) were readily isolated from Dutch coastal waters (southern North Sea) in 2000 and 2001. Our data show a large increase in the abundance of putative P. globosa viruses during blooms of P. globosa, suggesting that viruses are an important source of mortality for this alga. In order to examine genetic relatedness among viruses infecting P. globosa and other phytoplankton, DNA polymerase gene (pol) fragments were amplified and the inferred amino acid sequences were phylogenetically analyzed. The results demonstrated that viruses infecting P. globosa formed a closely related monophyletic group within the family Phycodnaviridae, with at least 96.9% similarity to each other. The sequences grouped most closely with others from viruses that infect the prymnesiophyte algae Chrysochromulina brevifilum and Chrysochromulina strobilus. Whether the P. globosa viruses belong to the genus Prymnesiovirus or form a separate group needs further study. Our data suggest that, like their phytoplankton hosts, the Chrysochromulina and Phaeocystis viruses share a common ancestor and that these prymnesioviruses and their algal host have coevolved.

Authors+Show Affiliations

Department of Biological Oceanography, Royal Netherlands Institute for Sea Research, P.O. Box 59, NL-1790 AB Den Burg, The Netherlands. corina.brussaard@nioz.nlNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15184176

Citation

Brussaard, C P D., et al. "Isolation and Phylogenetic Analysis of Novel Viruses Infecting the Phytoplankton Phaeocystis Globosa (Prymnesiophyceae)." Applied and Environmental Microbiology, vol. 70, no. 6, 2004, pp. 3700-5.
Brussaard CP, Short SM, Frederickson CM, et al. Isolation and phylogenetic analysis of novel viruses infecting the phytoplankton Phaeocystis globosa (Prymnesiophyceae). Appl Environ Microbiol. 2004;70(6):3700-5.
Brussaard, C. P., Short, S. M., Frederickson, C. M., & Suttle, C. A. (2004). Isolation and phylogenetic analysis of novel viruses infecting the phytoplankton Phaeocystis globosa (Prymnesiophyceae). Applied and Environmental Microbiology, 70(6), 3700-5.
Brussaard CP, et al. Isolation and Phylogenetic Analysis of Novel Viruses Infecting the Phytoplankton Phaeocystis Globosa (Prymnesiophyceae). Appl Environ Microbiol. 2004;70(6):3700-5. PubMed PMID: 15184176.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and phylogenetic analysis of novel viruses infecting the phytoplankton Phaeocystis globosa (Prymnesiophyceae). AU - Brussaard,C P D, AU - Short,S M, AU - Frederickson,C M, AU - Suttle,C A, PY - 2004/6/9/pubmed PY - 2004/8/11/medline PY - 2004/6/9/entrez SP - 3700 EP - 5 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 70 IS - 6 N2 - Viruses infecting the harmful bloom-causing alga Phaeocystis globosa (Prymnesiophyceae) were readily isolated from Dutch coastal waters (southern North Sea) in 2000 and 2001. Our data show a large increase in the abundance of putative P. globosa viruses during blooms of P. globosa, suggesting that viruses are an important source of mortality for this alga. In order to examine genetic relatedness among viruses infecting P. globosa and other phytoplankton, DNA polymerase gene (pol) fragments were amplified and the inferred amino acid sequences were phylogenetically analyzed. The results demonstrated that viruses infecting P. globosa formed a closely related monophyletic group within the family Phycodnaviridae, with at least 96.9% similarity to each other. The sequences grouped most closely with others from viruses that infect the prymnesiophyte algae Chrysochromulina brevifilum and Chrysochromulina strobilus. Whether the P. globosa viruses belong to the genus Prymnesiovirus or form a separate group needs further study. Our data suggest that, like their phytoplankton hosts, the Chrysochromulina and Phaeocystis viruses share a common ancestor and that these prymnesioviruses and their algal host have coevolved. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/15184176/Isolation_and_phylogenetic_analysis_of_novel_viruses_infecting_the_phytoplankton_Phaeocystis_globosa__Prymnesiophyceae__ L2 - https://journals.asm.org/doi/10.1128/AEM.70.6.3700-3705.2004?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -