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Dengue virus serotyping based on envelope and membrane and nonstructural protein NS1 serotype-specific capture immunoglobulin M enzyme-linked immunosorbent assays.
J Clin Microbiol. 2004 Jun; 42(6):2489-94.JC

Abstract

Envelope and membrane (E/M) and nonstructural protein NS1 serotype-specific capture Immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) were developed to differentiate four dengue virus serotypes. A total of 93 anti-dengue virus IgM-positive serum samples collected between days 5 and 45 of illness from 59 confirmed dengue patients were analyzed. The results showed that positive serotype specificity could be identified for 86.1 and 47.6% of serum samples tested for E/M-specific IgM antibodies versus 83.3 and 42.9% of serum samples tested for NS1-specific IgM antibodies from patients with primary and secondary dengue virus infections, respectively. Dual analyses with both E/M and NS1 serotype-specific capture IgM ELISAs showed that positive serotype specificity could be correctly identified for 98.6 and 61.9% of all of the primary and secondary serum samples tested, respectively. These findings suggested that E/M and NS1 serotype-specific capture IgM ELISAs have the potential to be of use in dengue virus serotyping.

Authors+Show Affiliations

Division of Laboratory Research and Development, Center for Disease Control, Department of Health, 161, Kun-Yang St., Taipei, Taiwan, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15184425

Citation

Shu, Pei-Yun, et al. "Dengue Virus Serotyping Based On Envelope and Membrane and Nonstructural Protein NS1 Serotype-specific Capture Immunoglobulin M Enzyme-linked Immunosorbent Assays." Journal of Clinical Microbiology, vol. 42, no. 6, 2004, pp. 2489-94.
Shu PY, Chen LK, Chang SF, et al. Dengue virus serotyping based on envelope and membrane and nonstructural protein NS1 serotype-specific capture immunoglobulin M enzyme-linked immunosorbent assays. J Clin Microbiol. 2004;42(6):2489-94.
Shu, P. Y., Chen, L. K., Chang, S. F., Su, C. L., Chien, L. J., Chin, C., Lin, T. H., & Huang, J. H. (2004). Dengue virus serotyping based on envelope and membrane and nonstructural protein NS1 serotype-specific capture immunoglobulin M enzyme-linked immunosorbent assays. Journal of Clinical Microbiology, 42(6), 2489-94.
Shu PY, et al. Dengue Virus Serotyping Based On Envelope and Membrane and Nonstructural Protein NS1 Serotype-specific Capture Immunoglobulin M Enzyme-linked Immunosorbent Assays. J Clin Microbiol. 2004;42(6):2489-94. PubMed PMID: 15184425.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dengue virus serotyping based on envelope and membrane and nonstructural protein NS1 serotype-specific capture immunoglobulin M enzyme-linked immunosorbent assays. AU - Shu,Pei-Yun, AU - Chen,Li-Kuang, AU - Chang,Shu-Fen, AU - Su,Chien-Ling, AU - Chien,Li-Jung, AU - Chin,Chuan, AU - Lin,Ting-Hsiang, AU - Huang,Jyh-Hsiung, PY - 2004/6/9/pubmed PY - 2004/7/30/medline PY - 2004/6/9/entrez SP - 2489 EP - 94 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 42 IS - 6 N2 - Envelope and membrane (E/M) and nonstructural protein NS1 serotype-specific capture Immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) were developed to differentiate four dengue virus serotypes. A total of 93 anti-dengue virus IgM-positive serum samples collected between days 5 and 45 of illness from 59 confirmed dengue patients were analyzed. The results showed that positive serotype specificity could be identified for 86.1 and 47.6% of serum samples tested for E/M-specific IgM antibodies versus 83.3 and 42.9% of serum samples tested for NS1-specific IgM antibodies from patients with primary and secondary dengue virus infections, respectively. Dual analyses with both E/M and NS1 serotype-specific capture IgM ELISAs showed that positive serotype specificity could be correctly identified for 98.6 and 61.9% of all of the primary and secondary serum samples tested, respectively. These findings suggested that E/M and NS1 serotype-specific capture IgM ELISAs have the potential to be of use in dengue virus serotyping. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/15184425/Dengue_virus_serotyping_based_on_envelope_and_membrane_and_nonstructural_protein_NS1_serotype_specific_capture_immunoglobulin_M_enzyme_linked_immunosorbent_assays_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=15184425 DB - PRIME DP - Unbound Medicine ER -