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Constitution of stable artificial oil bodies with triacylglycerol, phospholipid, and caleosin.
J Agric Food Chem. 2004 Jun 16; 52(12):3982-7.JA

Abstract

Seed oil bodies are lipid storage organelles of 0.5-2 microm in diameter and comprise a triacylglycerol matrix shielded by a monolayer of phospholipids and proteins. These proteins include abundant structural proteins, oleosins, and at least two minor proteins termed caleosin and steroleosin. This study examined if artificial oil bodies (AOBs) composed of triacylglycerol and phospholipid could be stabilized by oleosin, caleosin, or steroleosin. Our results showed that stabilization effects could be realized by oleosin or caleosin but not by steroleosin. The sizes of the AOBs constituted with oleosin (0.5-2 microm) or caleosin (50-200 nm) were similar to or 10 times smaller than those of the native oil bodies. Recombinant caleosin expressed in Escherichia coli also encapsulated AOBs with a size, topology, and stability comparable to those encapsulated with native caleosin. A proteinase K digestion indicated that caleosin anchored the AOBs via its central hydrophobic domain of approximately 4 kDa. Isoelectrofocusing revealed that the isoelectric point of the caleosin-stabilized AOBs was pH 4.0. Aggregation of AOBs was observed at a pH lower than 4.5; thus, their stability and integrity were presumably contributed by surface caleosin via electronegative repulsion and steric hindrance. The caleosin-stabilized AOBs were thermostable up to 70 degrees C and potentially useful for biotechnological applications.

Authors+Show Affiliations

Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15186126

Citation

Chen, Miles C M., et al. "Constitution of Stable Artificial Oil Bodies With Triacylglycerol, Phospholipid, and Caleosin." Journal of Agricultural and Food Chemistry, vol. 52, no. 12, 2004, pp. 3982-7.
Chen MC, Chyan CL, Lee TT, et al. Constitution of stable artificial oil bodies with triacylglycerol, phospholipid, and caleosin. J Agric Food Chem. 2004;52(12):3982-7.
Chen, M. C., Chyan, C. L., Lee, T. T., Huang, S. H., & Tzen, J. T. (2004). Constitution of stable artificial oil bodies with triacylglycerol, phospholipid, and caleosin. Journal of Agricultural and Food Chemistry, 52(12), 3982-7.
Chen MC, et al. Constitution of Stable Artificial Oil Bodies With Triacylglycerol, Phospholipid, and Caleosin. J Agric Food Chem. 2004 Jun 16;52(12):3982-7. PubMed PMID: 15186126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Constitution of stable artificial oil bodies with triacylglycerol, phospholipid, and caleosin. AU - Chen,Miles C M, AU - Chyan,Chia-Lin, AU - Lee,Tiger T T, AU - Huang,Shin-Hung, AU - Tzen,Jason T C, PY - 2004/6/10/pubmed PY - 2004/8/4/medline PY - 2004/6/10/entrez SP - 3982 EP - 7 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 52 IS - 12 N2 - Seed oil bodies are lipid storage organelles of 0.5-2 microm in diameter and comprise a triacylglycerol matrix shielded by a monolayer of phospholipids and proteins. These proteins include abundant structural proteins, oleosins, and at least two minor proteins termed caleosin and steroleosin. This study examined if artificial oil bodies (AOBs) composed of triacylglycerol and phospholipid could be stabilized by oleosin, caleosin, or steroleosin. Our results showed that stabilization effects could be realized by oleosin or caleosin but not by steroleosin. The sizes of the AOBs constituted with oleosin (0.5-2 microm) or caleosin (50-200 nm) were similar to or 10 times smaller than those of the native oil bodies. Recombinant caleosin expressed in Escherichia coli also encapsulated AOBs with a size, topology, and stability comparable to those encapsulated with native caleosin. A proteinase K digestion indicated that caleosin anchored the AOBs via its central hydrophobic domain of approximately 4 kDa. Isoelectrofocusing revealed that the isoelectric point of the caleosin-stabilized AOBs was pH 4.0. Aggregation of AOBs was observed at a pH lower than 4.5; thus, their stability and integrity were presumably contributed by surface caleosin via electronegative repulsion and steric hindrance. The caleosin-stabilized AOBs were thermostable up to 70 degrees C and potentially useful for biotechnological applications. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15186126/Constitution_of_stable_artificial_oil_bodies_with_triacylglycerol_phospholipid_and_caleosin_ L2 - https://doi.org/10.1021/jf035533g DB - PRIME DP - Unbound Medicine ER -