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Analysis of organic components of smokeless gunpowders: high-performance liquid chromatogaphy vs. micellar electrokinetic capillary chromatography.
Electrophoresis. 2004 Jun; 25(10-11):1543-7.E

Abstract

The aim of the present study was to verify the analytical performances of high-performance liquid chromatography (HPLC) and micellar electrokinetic capillary chromatography (MEKC) for the separation and qualitative determination of a selected group of organic components of smokeless gunpowders. The HPLC method was based on a gradient reversed-phase elution with a mobile phase composed of 0.17 M H(3)PO(4)/methanol; detection was performed by UV absorption at the wavelengths of 220, 254, and 270 nm. The MEKC experiments were carried out by using uncoated fused-silica capillaries (50 microm inside diameter, 50 cm effective length) and a running buffer composed of 10 mM sodium tetraborate at pH 9.24 added with 25 mM sodium dodecyl sulfate (SDS); the applied voltage was 25 kV; detection was either at a fixed wavelength UV of 214 nm or with a diode-array detector operating in the wavelength range from 190 to 350 nm. Both reversed-phase HPLC and MEKC techniques succeeded in resolving the tested standard mixtures of organic components of smokeless powders. Although the sequence of elution of the different analytes was slightly different between HPLC and MEKC, a statistical analysis based on the Spearman's rank correlation test showed that the two separation patterns were highly correlated. HPLC and MEKC were comparable in terms of elution/migration time precision, whereas MEKC showed higher reproducibility of peak areas. The interfacing of capillary electrophoresis with diode array UV detection provided distinct UV spectra of the individual analytes, thus improving, on the detection side, the analytical selectivity and identification power of capillary electrophoresis.

Authors+Show Affiliations

Department of Anatomy, Diagnostic Pathology, Legal Medicine, Hygiene and Public Heath, University of Catania, Catania, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15188239

Citation

Cascio, Orazio, et al. "Analysis of Organic Components of Smokeless Gunpowders: High-performance Liquid Chromatogaphy Vs. Micellar Electrokinetic Capillary Chromatography." Electrophoresis, vol. 25, no. 10-11, 2004, pp. 1543-7.
Cascio O, Trettene M, Bortolotti F, et al. Analysis of organic components of smokeless gunpowders: high-performance liquid chromatogaphy vs. micellar electrokinetic capillary chromatography. Electrophoresis. 2004;25(10-11):1543-7.
Cascio, O., Trettene, M., Bortolotti, F., Milana, G., & Tagliaro, F. (2004). Analysis of organic components of smokeless gunpowders: high-performance liquid chromatogaphy vs. micellar electrokinetic capillary chromatography. Electrophoresis, 25(10-11), 1543-7.
Cascio O, et al. Analysis of Organic Components of Smokeless Gunpowders: High-performance Liquid Chromatogaphy Vs. Micellar Electrokinetic Capillary Chromatography. Electrophoresis. 2004;25(10-11):1543-7. PubMed PMID: 15188239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of organic components of smokeless gunpowders: high-performance liquid chromatogaphy vs. micellar electrokinetic capillary chromatography. AU - Cascio,Orazio, AU - Trettene,Maristella, AU - Bortolotti,Federica, AU - Milana,Gabriella, AU - Tagliaro,Franco, PY - 2004/6/10/pubmed PY - 2004/12/28/medline PY - 2004/6/10/entrez SP - 1543 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 25 IS - 10-11 N2 - The aim of the present study was to verify the analytical performances of high-performance liquid chromatography (HPLC) and micellar electrokinetic capillary chromatography (MEKC) for the separation and qualitative determination of a selected group of organic components of smokeless gunpowders. The HPLC method was based on a gradient reversed-phase elution with a mobile phase composed of 0.17 M H(3)PO(4)/methanol; detection was performed by UV absorption at the wavelengths of 220, 254, and 270 nm. The MEKC experiments were carried out by using uncoated fused-silica capillaries (50 microm inside diameter, 50 cm effective length) and a running buffer composed of 10 mM sodium tetraborate at pH 9.24 added with 25 mM sodium dodecyl sulfate (SDS); the applied voltage was 25 kV; detection was either at a fixed wavelength UV of 214 nm or with a diode-array detector operating in the wavelength range from 190 to 350 nm. Both reversed-phase HPLC and MEKC techniques succeeded in resolving the tested standard mixtures of organic components of smokeless powders. Although the sequence of elution of the different analytes was slightly different between HPLC and MEKC, a statistical analysis based on the Spearman's rank correlation test showed that the two separation patterns were highly correlated. HPLC and MEKC were comparable in terms of elution/migration time precision, whereas MEKC showed higher reproducibility of peak areas. The interfacing of capillary electrophoresis with diode array UV detection provided distinct UV spectra of the individual analytes, thus improving, on the detection side, the analytical selectivity and identification power of capillary electrophoresis. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15188239/Analysis_of_organic_components_of_smokeless_gunpowders:_high_performance_liquid_chromatogaphy_vs__micellar_electrokinetic_capillary_chromatography_ DB - PRIME DP - Unbound Medicine ER -