Tags

Type your tag names separated by a space and hit enter

Determination of multiple drugs of abuse in human urine using capillary electrophoresis with fluorescence detection.
Electrophoresis. 2004 Jun; 25(10-11):1592-600.E

Abstract

Methods for separation and determination of multiple drugs of abuse in biological fluids using capillary electrophoresis (CE) with native fluorescence and laser-induced fluorescence (LIF) detection are described herein. Using native fluorescence, normorphine, morphine, 6-acetyl morphine (6-AM), and codeine were analyzed by CE without any derivatization procedure and detected at an excitation wavelength of 245 nm with a cut-off emission filter of 320 nm, providing a rapid and simple analysis. The detection limits were in the range of 200 ng/mL. For a highly sensitive analysis, LIF detection was also examined using a two-step precolumn derivatization procedure. In this case, drugs extracted from human urine were first subjected to an N-demethylation reaction involving the use of 1-chloroethyl chloroformate (ACE-Cl) and then derivatized using fluorescein isothiocyanate isomer I (FITC) and analyzed by CE coupled to a LIF detector. Variables affecting this derivatization: yield of demethylation reaction, FITC concentration, reaction time and temperature, were studied. The estimated instrumental detection limits of the FITC derivatives were in the range of 50-100 pg/mL, using LIF detection with excitation and emission wavelengths of 488 nm and 520 nm, respectively. The linearity, reproducibility and reliability of the methods were evaluated. In addition, a comparison of the characteristics for both native fluorescence and LIF detections was also discussed.

Authors+Show Affiliations

Department of Chemistry and Biochemistry, Clippinger Laboratories, Ohio University, Athens, OH 45701, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15188246

Citation

Alnajjar, Ahmed, et al. "Determination of Multiple Drugs of Abuse in Human Urine Using Capillary Electrophoresis With Fluorescence Detection." Electrophoresis, vol. 25, no. 10-11, 2004, pp. 1592-600.
Alnajjar A, Butcher JA, McCord B. Determination of multiple drugs of abuse in human urine using capillary electrophoresis with fluorescence detection. Electrophoresis. 2004;25(10-11):1592-600.
Alnajjar, A., Butcher, J. A., & McCord, B. (2004). Determination of multiple drugs of abuse in human urine using capillary electrophoresis with fluorescence detection. Electrophoresis, 25(10-11), 1592-600.
Alnajjar A, Butcher JA, McCord B. Determination of Multiple Drugs of Abuse in Human Urine Using Capillary Electrophoresis With Fluorescence Detection. Electrophoresis. 2004;25(10-11):1592-600. PubMed PMID: 15188246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of multiple drugs of abuse in human urine using capillary electrophoresis with fluorescence detection. AU - Alnajjar,Ahmed, AU - Butcher,Jared A, AU - McCord,Bruce, PY - 2004/6/10/pubmed PY - 2004/12/28/medline PY - 2004/6/10/entrez SP - 1592 EP - 600 JF - Electrophoresis JO - Electrophoresis VL - 25 IS - 10-11 N2 - Methods for separation and determination of multiple drugs of abuse in biological fluids using capillary electrophoresis (CE) with native fluorescence and laser-induced fluorescence (LIF) detection are described herein. Using native fluorescence, normorphine, morphine, 6-acetyl morphine (6-AM), and codeine were analyzed by CE without any derivatization procedure and detected at an excitation wavelength of 245 nm with a cut-off emission filter of 320 nm, providing a rapid and simple analysis. The detection limits were in the range of 200 ng/mL. For a highly sensitive analysis, LIF detection was also examined using a two-step precolumn derivatization procedure. In this case, drugs extracted from human urine were first subjected to an N-demethylation reaction involving the use of 1-chloroethyl chloroformate (ACE-Cl) and then derivatized using fluorescein isothiocyanate isomer I (FITC) and analyzed by CE coupled to a LIF detector. Variables affecting this derivatization: yield of demethylation reaction, FITC concentration, reaction time and temperature, were studied. The estimated instrumental detection limits of the FITC derivatives were in the range of 50-100 pg/mL, using LIF detection with excitation and emission wavelengths of 488 nm and 520 nm, respectively. The linearity, reproducibility and reliability of the methods were evaluated. In addition, a comparison of the characteristics for both native fluorescence and LIF detections was also discussed. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15188246/Determination_of_multiple_drugs_of_abuse_in_human_urine_using_capillary_electrophoresis_with_fluorescence_detection_ L2 - https://doi.org/10.1002/elps.200305847 DB - PRIME DP - Unbound Medicine ER -