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Infiltration by alien predators into invertebrate food webs in Hawaii: a molecular approach.
Mol Ecol. 2004 Jul; 13(7):2077-88.ME

Abstract

Abstract Alien invertebrate predators have been introduced to Hawaii to control pests, particularly in lowland areas where most crops are grown. We developed techniques for assessing the impact of these predators on native food webs in relatively pristine upland areas where, it was hypothesized, few lowland predators might be found. Predator densities were assessed along transects within the Alakaii Swamp on Kaua'i. The most numerous alien biocontrol agents found were Halmus chalybeus (Coccinellidae), a species known to feed on Lepidoptera eggs. Laboratory experiments were conducted using two genera of endemic Lepidoptera, Scotorythra and Eupithecia (Geometridae), that are of considerable conservation value, the former because of its recent speciation across Hawaii, the latter for its unique predatory larvae. Techniques were developed for detecting Lepidoptera DNA within the guts of alien predators using prey-specific PCR primers. General primers amplified fragments of the mitochondrial cytochrome oxidase I gene from beetles and Lepidoptera. The sequences were aligned and used successfully to design target-specific primers for general detection of the remains of Geometridae and for particular species, including Scotorythra rara and Eupithecia monticolans. DNA fragments amplified were short [140-170 base pairs (bp)], optimizing detection periods following prey ingestion. Trials using the introduced biocontrol agent Curinus coeruleus (Coccinellidae) demonstrated detection of Lepidoptera DNA fragments = 151 bp in 85-100% of beetles after 24 h digestion of an early instar larva. This study provides a framework for future use of molecular gut analysis in arthropod conservation ecology and food web research with considerable potential for quantifying threats to endemic species in Hawaii and elsewhere.

Authors+Show Affiliations

Cardiff School of Biosciences, Cardiff University, P.O.Box 915, Cardiff CF10 3TL, UK. SheppardSK1@Cardiff.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15189227

Citation

Sheppard, S K., et al. "Infiltration By Alien Predators Into Invertebrate Food Webs in Hawaii: a Molecular Approach." Molecular Ecology, vol. 13, no. 7, 2004, pp. 2077-88.
Sheppard SK, Henneman ML, Memmott J, et al. Infiltration by alien predators into invertebrate food webs in Hawaii: a molecular approach. Mol Ecol. 2004;13(7):2077-88.
Sheppard, S. K., Henneman, M. L., Memmott, J., & Symondson, W. O. (2004). Infiltration by alien predators into invertebrate food webs in Hawaii: a molecular approach. Molecular Ecology, 13(7), 2077-88.
Sheppard SK, et al. Infiltration By Alien Predators Into Invertebrate Food Webs in Hawaii: a Molecular Approach. Mol Ecol. 2004;13(7):2077-88. PubMed PMID: 15189227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Infiltration by alien predators into invertebrate food webs in Hawaii: a molecular approach. AU - Sheppard,S K, AU - Henneman,M L, AU - Memmott,J, AU - Symondson,W O C, PY - 2004/6/11/pubmed PY - 2005/1/26/medline PY - 2004/6/11/entrez SP - 2077 EP - 88 JF - Molecular ecology JO - Mol. Ecol. VL - 13 IS - 7 N2 - Abstract Alien invertebrate predators have been introduced to Hawaii to control pests, particularly in lowland areas where most crops are grown. We developed techniques for assessing the impact of these predators on native food webs in relatively pristine upland areas where, it was hypothesized, few lowland predators might be found. Predator densities were assessed along transects within the Alakaii Swamp on Kaua'i. The most numerous alien biocontrol agents found were Halmus chalybeus (Coccinellidae), a species known to feed on Lepidoptera eggs. Laboratory experiments were conducted using two genera of endemic Lepidoptera, Scotorythra and Eupithecia (Geometridae), that are of considerable conservation value, the former because of its recent speciation across Hawaii, the latter for its unique predatory larvae. Techniques were developed for detecting Lepidoptera DNA within the guts of alien predators using prey-specific PCR primers. General primers amplified fragments of the mitochondrial cytochrome oxidase I gene from beetles and Lepidoptera. The sequences were aligned and used successfully to design target-specific primers for general detection of the remains of Geometridae and for particular species, including Scotorythra rara and Eupithecia monticolans. DNA fragments amplified were short [140-170 base pairs (bp)], optimizing detection periods following prey ingestion. Trials using the introduced biocontrol agent Curinus coeruleus (Coccinellidae) demonstrated detection of Lepidoptera DNA fragments = 151 bp in 85-100% of beetles after 24 h digestion of an early instar larva. This study provides a framework for future use of molecular gut analysis in arthropod conservation ecology and food web research with considerable potential for quantifying threats to endemic species in Hawaii and elsewhere. SN - 0962-1083 UR - https://www.unboundmedicine.com/medline/citation/15189227/Infiltration_by_alien_predators_into_invertebrate_food_webs_in_Hawaii:_a_molecular_approach_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0962-1083&date=2004&volume=13&issue=7&spage=2077 DB - PRIME DP - Unbound Medicine ER -