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A PCR-based method that permits specific detection of Paenibacillus larvae subsp. larvae, the cause of American Foulbrood of honey bees, at the subspecies level.
Lett Appl Microbiol. 2004; 39(1):25-33.LA

Abstract

AIMS

A reliable procedure for the identification of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood disease of honey bees (Apis mellifera L.) based on the polymerase chain reaction (PCR) and subspecies - specific primers is described.

METHODS AND RESULTS

By using ERIC-PCR, an amplicon of ca 970 bp was found among P. l. larvae strains but not in other closely related species. Based on the nucleotide sequence data of this amplicon, we designed the pair of oligonucleotides KAT 1 and KAT 2, which were assayed as primers in a PCR reaction. A PCR amplicon of the expected size ca 550 bp was only found in P. l. larvae strains.

CONCLUSIONS

This PCR assay provides a specific detection for P. l. larvae.

SIGNIFICANCE AND IMPACT OF THE STUDY

The developed PCR assay is highly specific because can differentiate Paenibacillus larvae subsp. larvae from the closely related Paenibacillus larvae subsp. pulvifaciens. The technique can be directly used to detect presence or absence of P. l. larvae spores in honey bee brood samples and contaminated honeys.

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Authors+Show Affiliations

Alippi AM
Facultad de Ciencias Agrarias y Forestales, Centro de Investigaciones de Fitopatología (CIDEFI), Universidad Nacional de La Plata, La Plata, Argentina. alippi@biol.unlp.edu.ar
López AC
No affiliation info available
Aguilar OM
No affiliation info available

MeSH

AnimalsBacillaceaeBeesDNA PrimersHoneyLarvaPolymerase Chain ReactionSpecies SpecificitySpores, Bacterial

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15189284

Citation

Alippi, A M., et al. "A PCR-based Method That Permits Specific Detection of Paenibacillus Larvae Subsp. Larvae, the Cause of American Foulbrood of Honey Bees, at the Subspecies Level." Letters in Applied Microbiology, vol. 39, no. 1, 2004, pp. 25-33.
Alippi AM, López AC, Aguilar OM. A PCR-based method that permits specific detection of Paenibacillus larvae subsp. larvae, the cause of American Foulbrood of honey bees, at the subspecies level. Lett Appl Microbiol. 2004;39(1):25-33.
Alippi, A. M., López, A. C., & Aguilar, O. M. (2004). A PCR-based method that permits specific detection of Paenibacillus larvae subsp. larvae, the cause of American Foulbrood of honey bees, at the subspecies level. Letters in Applied Microbiology, 39(1), 25-33.
Alippi AM, López AC, Aguilar OM. A PCR-based Method That Permits Specific Detection of Paenibacillus Larvae Subsp. Larvae, the Cause of American Foulbrood of Honey Bees, at the Subspecies Level. Lett Appl Microbiol. 2004;39(1):25-33. PubMed PMID: 15189284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A PCR-based method that permits specific detection of Paenibacillus larvae subsp. larvae, the cause of American Foulbrood of honey bees, at the subspecies level. AU - Alippi,A M, AU - López,A C, AU - Aguilar,O M, PY - 2004/6/11/pubmed PY - 2004/9/4/medline PY - 2004/6/11/entrez SP - 25 EP - 33 JF - Letters in applied microbiology JO - Lett Appl Microbiol VL - 39 IS - 1 N2 - AIMS: A reliable procedure for the identification of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood disease of honey bees (Apis mellifera L.) based on the polymerase chain reaction (PCR) and subspecies - specific primers is described. METHODS AND RESULTS: By using ERIC-PCR, an amplicon of ca 970 bp was found among P. l. larvae strains but not in other closely related species. Based on the nucleotide sequence data of this amplicon, we designed the pair of oligonucleotides KAT 1 and KAT 2, which were assayed as primers in a PCR reaction. A PCR amplicon of the expected size ca 550 bp was only found in P. l. larvae strains. CONCLUSIONS: This PCR assay provides a specific detection for P. l. larvae. SIGNIFICANCE AND IMPACT OF THE STUDY: The developed PCR assay is highly specific because can differentiate Paenibacillus larvae subsp. larvae from the closely related Paenibacillus larvae subsp. pulvifaciens. The technique can be directly used to detect presence or absence of P. l. larvae spores in honey bee brood samples and contaminated honeys. SN - 0266-8254 UR - https://www.unboundmedicine.com/medline/citation/15189284/A_PCR_based_method_that_permits_specific_detection_of_Paenibacillus_larvae_subsp__larvae_the_cause_of_American_Foulbrood_of_honey_bees_at_the_subspecies_level_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0266-8254&date=2004&volume=39&issue=1&spage=25 DB - PRIME DP - Unbound Medicine ER -