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[Construction of human phage-displayed scFv library and selection of the ScFv against rabies virus].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Mar; 20(2):243-7.XB

Abstract

AIM

To construct a human phage displayed single chain Fv antibody (scFv) library and screen specific scFv against rabies virus.

METHODS

Using a phage-display technique, IgVH and IgVL genes were cloned from peripheral blood lymphocytes from three donors immunized with the WISTAR PM strain vaccine. Genes encoding scFv fragments were constructed by random linkage of VH gene and VL gene by SOE PCR, and then the constructed scFv genes were cloned into phagemid vector and transfected into E. coli TG1. The recombinant phages were screened by four rounds of panning with rabies virus vaccine. The positive recombinant phages were sequenced and antigen-binding activity of recombinant scFv was detected by competition ELISA.

RESULTS

A human phage-displayed scFv library with about 7 x 10(8) sink size was constructed. A new human scFv was selected, which bound specifically to rabies virus and had higher affinity.

CONCLUSION

The results lay a solid foundation for preparation of human engineering antibody to rabies virus reported herein with higher affinity.

Authors+Show Affiliations

Institute of Microbiology and Epidemiology, the Academy of Military Medical Sciences, Beijing 100071, China. xlzhao985@sohu.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

15191736

Citation

Zhao, Xiao-ling, et al. "[Construction of Human Phage-displayed scFv Library and Selection of the ScFv Against Rabies Virus]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 20, no. 2, 2004, pp. 243-7.
Zhao XL, Yin J, Wang H, et al. [Construction of human phage-displayed scFv library and selection of the ScFv against rabies virus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004;20(2):243-7.
Zhao, X. L., Yin, J., Wang, H., Jiang, M., & Hou, X. J. (2004). [Construction of human phage-displayed scFv library and selection of the ScFv against rabies virus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 20(2), 243-7.
Zhao XL, et al. [Construction of Human Phage-displayed scFv Library and Selection of the ScFv Against Rabies Virus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004;20(2):243-7. PubMed PMID: 15191736.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Construction of human phage-displayed scFv library and selection of the ScFv against rabies virus]. AU - Zhao,Xiao-ling, AU - Yin,Jun, AU - Wang,Hui, AU - Jiang,Ming, AU - Hou,Xiao-jun, PY - 2004/6/12/pubmed PY - 2006/4/1/medline PY - 2004/6/12/entrez SP - 243 EP - 7 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 20 IS - 2 N2 - AIM: To construct a human phage displayed single chain Fv antibody (scFv) library and screen specific scFv against rabies virus. METHODS: Using a phage-display technique, IgVH and IgVL genes were cloned from peripheral blood lymphocytes from three donors immunized with the WISTAR PM strain vaccine. Genes encoding scFv fragments were constructed by random linkage of VH gene and VL gene by SOE PCR, and then the constructed scFv genes were cloned into phagemid vector and transfected into E. coli TG1. The recombinant phages were screened by four rounds of panning with rabies virus vaccine. The positive recombinant phages were sequenced and antigen-binding activity of recombinant scFv was detected by competition ELISA. RESULTS: A human phage-displayed scFv library with about 7 x 10(8) sink size was constructed. A new human scFv was selected, which bound specifically to rabies virus and had higher affinity. CONCLUSION: The results lay a solid foundation for preparation of human engineering antibody to rabies virus reported herein with higher affinity. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/15191736/[Construction_of_human_phage_displayed_scFv_library_and_selection_of_the_ScFv_against_rabies_virus]_ L2 - http://www.diseaseinfosearch.org/result/6131 DB - PRIME DP - Unbound Medicine ER -