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Expression pattern of UidA gene under the control of rice glutelin GluA-2 gene upstream sequence in transgenic rice endosperm.
Yi Chuan Xue Bao. 2004 Mar; 31(3):281-6.YC

Abstract

In order to study the expression pattern of rice glutelin endosperm specific promoter in Chinese cultivar Zhonghua 8 (Oryza sativa L. subsp japonica), UidA gene was fused with rice glutelin GluA-2 gene 5' upstream sequence 2.3 kb and 750 bp upstream respectively and transferred into rice by Agrobacterium mediated transformation. Southern blot indicated that UidA gene was integrated into the genome of transgenic plants as single copy. Northern blot demonstrated that the expression of UidA gene and endogenous GluA-2 gene reached their highest level at 13-15 days and 11-13 days after pollination respectively, and then declined. Histochemical staining of immature transgenic rice seeds showed UidA gene was specifically expressed in endosperm and the highest level GUS expression was observed in aleurone layer. Quantitative analysis of GUS activity showed seeds GUS activity of that 2.3 kb transgenic plant was about two to three folds of those of 750 bp transgenic plant. Sequence analysis suggested that the G-box located in the -2,170 bp (from transcription start site) may be a quantitative cis-element.

Authors+Show Affiliations

Chen Y
State Key Laboratory of Plant Cell & Chromosome Engineering, Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing 100101, China.
Qu LQ
No affiliation info available
Jia X
No affiliation info available

MeSH

Blotting, SouthernGene Expression ProfilingGene Expression Regulation, PlantGenes, PlantGlutensOryzaPlants, Genetically Modified

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15195568

Citation

Chen, Yu, et al. "Expression Pattern of UidA Gene Under the Control of Rice Glutelin GluA-2 Gene Upstream Sequence in Transgenic Rice Endosperm." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 31, no. 3, 2004, pp. 281-6.
Chen Y, Qu LQ, Jia X. Expression pattern of UidA gene under the control of rice glutelin GluA-2 gene upstream sequence in transgenic rice endosperm. Yi Chuan Xue Bao. 2004;31(3):281-6.
Chen, Y., Qu, L. Q., & Jia, X. (2004). Expression pattern of UidA gene under the control of rice glutelin GluA-2 gene upstream sequence in transgenic rice endosperm. Yi Chuan Xue Bao = Acta Genetica Sinica, 31(3), 281-6.
Chen Y, Qu LQ, Jia X. Expression Pattern of UidA Gene Under the Control of Rice Glutelin GluA-2 Gene Upstream Sequence in Transgenic Rice Endosperm. Yi Chuan Xue Bao. 2004;31(3):281-6. PubMed PMID: 15195568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression pattern of UidA gene under the control of rice glutelin GluA-2 gene upstream sequence in transgenic rice endosperm. AU - Chen,Yu, AU - Qu,Le-Qing, AU - Jia,Xu, PY - 2004/6/16/pubmed PY - 2004/7/1/medline PY - 2004/6/16/entrez SP - 281 EP - 6 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 31 IS - 3 N2 - In order to study the expression pattern of rice glutelin endosperm specific promoter in Chinese cultivar Zhonghua 8 (Oryza sativa L. subsp japonica), UidA gene was fused with rice glutelin GluA-2 gene 5' upstream sequence 2.3 kb and 750 bp upstream respectively and transferred into rice by Agrobacterium mediated transformation. Southern blot indicated that UidA gene was integrated into the genome of transgenic plants as single copy. Northern blot demonstrated that the expression of UidA gene and endogenous GluA-2 gene reached their highest level at 13-15 days and 11-13 days after pollination respectively, and then declined. Histochemical staining of immature transgenic rice seeds showed UidA gene was specifically expressed in endosperm and the highest level GUS expression was observed in aleurone layer. Quantitative analysis of GUS activity showed seeds GUS activity of that 2.3 kb transgenic plant was about two to three folds of those of 750 bp transgenic plant. Sequence analysis suggested that the G-box located in the -2,170 bp (from transcription start site) may be a quantitative cis-element. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/15195568/Expression_pattern_of_UidA_gene_under_the_control_of_rice_glutelin_GluA_2_gene_upstream_sequence_in_transgenic_rice_endosperm_ DB - PRIME DP - Unbound Medicine ER -