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Interleukin-18 levels in induced sputum are reduced in asthmatic and normal smokers.
Clin Exp Allergy. 2004 Jun; 34(6):904-10.CE

Abstract

BACKGROUND

IL-18 is a cytokine which is known to have an important role in the development of a Th1 lymphocyte response. As such, it may have a regulatory role in asthma by modifying Th2 lymphocyte responses. Cigarette smoking may amplify the airway inflammation associated with asthma.

OBJECTIVE

This study investigated if IL-18 could be detected in induced sputum from asthmatics and normal subjects and if smoking altered IL-18 levels.

METHODS

Induced sputum was obtained from asthmatic (31 smokers, 35 non-smokers) and normal (20 smokers, 20 non-smokers) subjects. All smokers had a smoking history of > or =15 pack years. IL-18 levels in sputum supernatant were measured by ELISA. IL-18 mRNA expression and cellular localization were assessed by quantitative PCR and immunocytochemistry, respectively.

RESULTS

Smoking was associated with a significant reduction in IL-18 levels (median (interquartile range) - smokers 20 (0-102) pg/mL vs. non-smokers 358 (50-876) pg/mL, P<0.001). This was more pronounced in asthmatics (smokers, 47 (40-64) pg/mL vs. non-smokers, 530 (30-1484) pg/mL; P<0.001) than in normal subjects (smokers, 25 (0-78) pg/mL vs. non-smokers, 247 (50-656) pg/mL; P<0.01). Within each of the smoking and non-smoking groups there was no significant difference in IL-18 levels between asthmatic and normal subjects. There was no correlation between sputum IL-18 levels and any specific cell type in the sputum samples nor serum IgE levels. IL-18 mRNA expression was reduced in asthmatic smokers compared with non-smokers. IL-18 production was localized to sputum macrophages by immunocytochemistry.

CONCLUSIONS

IL-18 is detectable in induced sputum samples from both asthmatic and normal subjects. Cigarette smoking significantly reduces sputum IL-18 levels. This effect is more pronounced in asthmatics than in normal subjects.

Authors+Show Affiliations

Division of Immunology, Infection and Inflammation, University of Glasgow, Glasgow, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15196278

Citation

McKay, A, et al. "Interleukin-18 Levels in Induced Sputum Are Reduced in Asthmatic and Normal Smokers." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 34, no. 6, 2004, pp. 904-10.
McKay A, Komai-Koma M, MacLeod KJ, et al. Interleukin-18 levels in induced sputum are reduced in asthmatic and normal smokers. Clin Exp Allergy. 2004;34(6):904-10.
McKay, A., Komai-Koma, M., MacLeod, K. J., Campbell, C. C., Kitson, S. M., Chaudhuri, R., Thomson, L., McSharry, C., Liew, F. Y., & Thomson, N. C. (2004). Interleukin-18 levels in induced sputum are reduced in asthmatic and normal smokers. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 34(6), 904-10.
McKay A, et al. Interleukin-18 Levels in Induced Sputum Are Reduced in Asthmatic and Normal Smokers. Clin Exp Allergy. 2004;34(6):904-10. PubMed PMID: 15196278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interleukin-18 levels in induced sputum are reduced in asthmatic and normal smokers. AU - McKay,A, AU - Komai-Koma,M, AU - MacLeod,K J, AU - Campbell,C C, AU - Kitson,S M, AU - Chaudhuri,R, AU - Thomson,L, AU - McSharry,C, AU - Liew,F Y, AU - Thomson,N C, PY - 2004/6/16/pubmed PY - 2004/9/21/medline PY - 2004/6/16/entrez SP - 904 EP - 10 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 34 IS - 6 N2 - BACKGROUND: IL-18 is a cytokine which is known to have an important role in the development of a Th1 lymphocyte response. As such, it may have a regulatory role in asthma by modifying Th2 lymphocyte responses. Cigarette smoking may amplify the airway inflammation associated with asthma. OBJECTIVE: This study investigated if IL-18 could be detected in induced sputum from asthmatics and normal subjects and if smoking altered IL-18 levels. METHODS: Induced sputum was obtained from asthmatic (31 smokers, 35 non-smokers) and normal (20 smokers, 20 non-smokers) subjects. All smokers had a smoking history of > or =15 pack years. IL-18 levels in sputum supernatant were measured by ELISA. IL-18 mRNA expression and cellular localization were assessed by quantitative PCR and immunocytochemistry, respectively. RESULTS: Smoking was associated with a significant reduction in IL-18 levels (median (interquartile range) - smokers 20 (0-102) pg/mL vs. non-smokers 358 (50-876) pg/mL, P<0.001). This was more pronounced in asthmatics (smokers, 47 (40-64) pg/mL vs. non-smokers, 530 (30-1484) pg/mL; P<0.001) than in normal subjects (smokers, 25 (0-78) pg/mL vs. non-smokers, 247 (50-656) pg/mL; P<0.01). Within each of the smoking and non-smoking groups there was no significant difference in IL-18 levels between asthmatic and normal subjects. There was no correlation between sputum IL-18 levels and any specific cell type in the sputum samples nor serum IgE levels. IL-18 mRNA expression was reduced in asthmatic smokers compared with non-smokers. IL-18 production was localized to sputum macrophages by immunocytochemistry. CONCLUSIONS: IL-18 is detectable in induced sputum samples from both asthmatic and normal subjects. Cigarette smoking significantly reduces sputum IL-18 levels. This effect is more pronounced in asthmatics than in normal subjects. SN - 0954-7894 UR - https://www.unboundmedicine.com/medline/citation/15196278/Interleukin_18_levels_in_induced_sputum_are_reduced_in_asthmatic_and_normal_smokers_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=0954-7894&amp;date=2004&amp;volume=34&amp;issue=6&amp;spage=904 DB - PRIME DP - Unbound Medicine ER -