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[Effect of lung protective ventilation strategy on pulmonary inflammatory response in a rabbit model of acute respiratory distress syndrome].
Zhonghua Jie He He Hu Xi Za Zhi. 2004 May; 27(5):298-301.ZJ

Abstract

OBJECTIVE

To evaluate the effect of lung protective ventilation strategy on pulmonary inflammatory response in acute respiratory distress syndrome (ARDS).

METHODS

The ARDS rabbit model was duplicated by saline alveolar-lavage. The rabbits were divided into six groups: (1) normal control group (N group); (2) ARDS group (M group); (3) low-volume with best end-expiratory pressure (PEEP, A group) group: tidal volume (V(T)) 6 ml/kg, PEEP 2 cm H(2)O greater than the pressure of lower inflection point in pressure-volume curve (P(LIP)); (4) normal-volume with best PEEP group (B group): V(T) 6 ml/kg, and PEEP P(LIP) + 2 cm H(2)O; (5) low-volume with high PEEP group (C group): V(T) 6 ml/kg, and PEEP 15 cm H(2)O; and (6) high-volume with zero PEEP group (D group): V(T) 20 ml/kg. Lung wet/dry weight ratios (W/D) were recorded to evaluate lung injury. After 4 h of ventilation, lung homogenates were prepared to detect nuclear factor-kappaB (NF-kappaB) activity by electrophoretic mobility gel shift assay (EMSA), tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) levels by enzyme-linked immunosorbent assay (ELISA) and their mRNA expression by reverse transcriptase-polymerase chain reaction (RT-PCR). Myeloperoxidase (MPO) and malondialdehyde (MDA) in lung homogenates were also assessed.

RESULTS

After 4 h ventilation, W/D in A group (5.6 +/- 1.1) were significantly lower than those in B group, C group and D group (6.6 +/- 0.8, 6.6 +/- 1.0, 6.9 +/- 1.0, all P < 0.05). But there was no difference between A group and M group (5.8 +/- 0.5). NF-kappaB activity was the highest in D group, and that in A group was 331 +/- 113, which was decreased significantly as compared with B, C and D groups (455 +/- 63, 478 +/- 74, 645 +/- 162, all P < 0.05). The mRNA expression of TNF-alpha and IL-10 and their concentrations in lung homogenates in A group were lower than those in B, C and D groups. In A group, the concentrations of MPO and MDA in lung homogenates were significantly lower than those in B, C and D groups.

CONCLUSION

Lung protective ventilation strategy can inhibit lung inflammation and may improve lung injury in ARDS, but low tidal volume with high PEEP may increase lung inflammation.

Authors+Show Affiliations

Department of Critical Care Medicine, Zhong-Da Hospital and School of Clinical Medicine, Southeast University, Nanjing 210009, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15196336

Citation

Qiu, Hai-bo, et al. "[Effect of Lung Protective Ventilation Strategy On Pulmonary Inflammatory Response in a Rabbit Model of Acute Respiratory Distress Syndrome]." Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, vol. 27, no. 5, 2004, pp. 298-301.
Qiu HB, Yan YL, Yang Y, et al. [Effect of lung protective ventilation strategy on pulmonary inflammatory response in a rabbit model of acute respiratory distress syndrome]. Zhonghua Jie He He Hu Xi Za Zhi. 2004;27(5):298-301.
Qiu, H. B., Yan, Y. L., Yang, Y., Zhou, S. X., Xu, H. Y., & Wang, L. (2004). [Effect of lung protective ventilation strategy on pulmonary inflammatory response in a rabbit model of acute respiratory distress syndrome]. Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, 27(5), 298-301.
Qiu HB, et al. [Effect of Lung Protective Ventilation Strategy On Pulmonary Inflammatory Response in a Rabbit Model of Acute Respiratory Distress Syndrome]. Zhonghua Jie He He Hu Xi Za Zhi. 2004;27(5):298-301. PubMed PMID: 15196336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of lung protective ventilation strategy on pulmonary inflammatory response in a rabbit model of acute respiratory distress syndrome]. AU - Qiu,Hai-bo, AU - Yan,Yan-li, AU - Yang,Yi, AU - Zhou,Shao-xia, AU - Xu,Hong-yang, AU - Wang,Li, PY - 2004/6/16/pubmed PY - 2006/7/22/medline PY - 2004/6/16/entrez SP - 298 EP - 301 JF - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases JO - Zhonghua Jie He He Hu Xi Za Zhi VL - 27 IS - 5 N2 - OBJECTIVE: To evaluate the effect of lung protective ventilation strategy on pulmonary inflammatory response in acute respiratory distress syndrome (ARDS). METHODS: The ARDS rabbit model was duplicated by saline alveolar-lavage. The rabbits were divided into six groups: (1) normal control group (N group); (2) ARDS group (M group); (3) low-volume with best end-expiratory pressure (PEEP, A group) group: tidal volume (V(T)) 6 ml/kg, PEEP 2 cm H(2)O greater than the pressure of lower inflection point in pressure-volume curve (P(LIP)); (4) normal-volume with best PEEP group (B group): V(T) 6 ml/kg, and PEEP P(LIP) + 2 cm H(2)O; (5) low-volume with high PEEP group (C group): V(T) 6 ml/kg, and PEEP 15 cm H(2)O; and (6) high-volume with zero PEEP group (D group): V(T) 20 ml/kg. Lung wet/dry weight ratios (W/D) were recorded to evaluate lung injury. After 4 h of ventilation, lung homogenates were prepared to detect nuclear factor-kappaB (NF-kappaB) activity by electrophoretic mobility gel shift assay (EMSA), tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) levels by enzyme-linked immunosorbent assay (ELISA) and their mRNA expression by reverse transcriptase-polymerase chain reaction (RT-PCR). Myeloperoxidase (MPO) and malondialdehyde (MDA) in lung homogenates were also assessed. RESULTS: After 4 h ventilation, W/D in A group (5.6 +/- 1.1) were significantly lower than those in B group, C group and D group (6.6 +/- 0.8, 6.6 +/- 1.0, 6.9 +/- 1.0, all P < 0.05). But there was no difference between A group and M group (5.8 +/- 0.5). NF-kappaB activity was the highest in D group, and that in A group was 331 +/- 113, which was decreased significantly as compared with B, C and D groups (455 +/- 63, 478 +/- 74, 645 +/- 162, all P < 0.05). The mRNA expression of TNF-alpha and IL-10 and their concentrations in lung homogenates in A group were lower than those in B, C and D groups. In A group, the concentrations of MPO and MDA in lung homogenates were significantly lower than those in B, C and D groups. CONCLUSION: Lung protective ventilation strategy can inhibit lung inflammation and may improve lung injury in ARDS, but low tidal volume with high PEEP may increase lung inflammation. SN - 1001-0939 UR - https://www.unboundmedicine.com/medline/citation/15196336/[Effect_of_lung_protective_ventilation_strategy_on_pulmonary_inflammatory_response_in_a_rabbit_model_of_acute_respiratory_distress_syndrome]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=1001-0939&amp;year=2004&amp;vol=27&amp;issue=5&amp;fpage=298 DB - PRIME DP - Unbound Medicine ER -