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Surface plasmon resonance immunosensor using self-assembled protein G for the detection of Salmonella paratyphi.
J Biotechnol. 2004 Jul 01; 111(1):1-8.JB

Abstract

A surface plasmon resonance (SPR) based immunosensor using self-assembled protein G was developed for the detection of Salmonella paratyphi. In order to endow a solid substrate binding affinity to protein G, the free amine (-NH2) of protein G was substituted into thiol (-SH) using 2-iminothiolane. Thus, self-assembled protein G was fabricated on gold (Au) substrate. The formation of protein G layer on Au surface, and the binding of antibody and antigen in series were confirmed by SPR spectroscopy. The surface morphology analysis of the protein G layer on Au surface was performed by atomic force microscope (AFM). Consequently, an immunosensor based on SPR for the detection of S. paratyphi using self-assembled protein G was developed with a detection range of 10(2)-10(7) CFU/ml. The current fabrication technique of a SPR immunosensor for the detection of S. paratyphi could be applied to construct other immnosensors or protein chips.

Authors+Show Affiliations

Oh BK
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Shinsu-dong, Mapo-gu, Seoul 121-742, South Korea.
Lee W
No affiliation info available
Kim YK
No affiliation info available
Lee WH
No affiliation info available
Choi JW
No affiliation info available

MeSH

Biosensing TechniquesCoated Materials, BiocompatibleColony Count, MicrobialCrystallizationEquipment DesignEquipment Failure AnalysisImmunoassayMultiprotein ComplexesNerve Tissue ProteinsSalmonella paratyphi ASurface Plasmon Resonance

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15196764

Citation

Oh, Byung-Keun, et al. "Surface Plasmon Resonance Immunosensor Using Self-assembled Protein G for the Detection of Salmonella Paratyphi." Journal of Biotechnology, vol. 111, no. 1, 2004, pp. 1-8.
Oh BK, Lee W, Kim YK, et al. Surface plasmon resonance immunosensor using self-assembled protein G for the detection of Salmonella paratyphi. J Biotechnol. 2004;111(1):1-8.
Oh, B. K., Lee, W., Kim, Y. K., Lee, W. H., & Choi, J. W. (2004). Surface plasmon resonance immunosensor using self-assembled protein G for the detection of Salmonella paratyphi. Journal of Biotechnology, 111(1), 1-8.
Oh BK, et al. Surface Plasmon Resonance Immunosensor Using Self-assembled Protein G for the Detection of Salmonella Paratyphi. J Biotechnol. 2004 Jul 1;111(1):1-8. PubMed PMID: 15196764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface plasmon resonance immunosensor using self-assembled protein G for the detection of Salmonella paratyphi. AU - Oh,Byung-Keun, AU - Lee,Woochang, AU - Kim,Young-Kee, AU - Lee,Won Hong, AU - Choi,Jeong-Woo, PY - 2003/09/10/received PY - 2004/01/21/revised PY - 2004/02/12/accepted PY - 2004/6/16/pubmed PY - 2005/2/11/medline PY - 2004/6/16/entrez SP - 1 EP - 8 JF - Journal of biotechnology JO - J Biotechnol VL - 111 IS - 1 N2 - A surface plasmon resonance (SPR) based immunosensor using self-assembled protein G was developed for the detection of Salmonella paratyphi. In order to endow a solid substrate binding affinity to protein G, the free amine (-NH2) of protein G was substituted into thiol (-SH) using 2-iminothiolane. Thus, self-assembled protein G was fabricated on gold (Au) substrate. The formation of protein G layer on Au surface, and the binding of antibody and antigen in series were confirmed by SPR spectroscopy. The surface morphology analysis of the protein G layer on Au surface was performed by atomic force microscope (AFM). Consequently, an immunosensor based on SPR for the detection of S. paratyphi using self-assembled protein G was developed with a detection range of 10(2)-10(7) CFU/ml. The current fabrication technique of a SPR immunosensor for the detection of S. paratyphi could be applied to construct other immnosensors or protein chips. SN - 0168-1656 UR - https://www.unboundmedicine.com/medline/citation/15196764/Surface_plasmon_resonance_immunosensor_using_self_assembled_protein_G_for_the_detection_of_Salmonella_paratyphi_ DB - PRIME DP - Unbound Medicine ER -