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Genomic constitution and variation in five partial amphiploids of wheat--Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis.
Theor Appl Genet. 2004 Sep; 109(5):1070-6.TA

Abstract

Genomic in situ hybridization (GISH) and multicolor GISH (mcGISH) methodology were used to establish the cytogenetic constitution of five partial amphiploid lines obtained from wheat x Thinopyrum intermedium hybridizations. Line Zhong 1, 2 n=52, contained 14 chromosomes from each of the wheat genomes plus ten Th. intermedium chromosomes, with one pair of A-genome chromosomes having a Th. intermedium chromosomal segment translocated to the short arm. Line Zhong 2, 2 n=54, had intact ABD wheat genome chromosomes plus 12 Th. intermedium chromosomes. The multicolor GISH results, using different fluorochrome labeled Th. intermedium and the various diploid wheat genomic DNAs as probes, indicated that both Zhong 1 and Zhong 2 contained one pair of Th. intermedium chromosomes with a significant homology to the wheat D genome. High-molecular-weight (HMW) glutenin and gliadin analysis revealed that Zhong 1 and Zhong 2 had identical banding patterns that contained all of the wheat bands and a specific HMW band from Th. intermedium. Zhong 1 and Zhong 2 had good HMW subunits for wheat breeding. Zhong 3 and Zhong 5, both 2 n=56, possessed no gross chromosomal aberrations or translocations that were detectable at the GISH level. Zhong 4 also had a chromosome number of 2 n=56 and contained the complete wheat ABD-genome chromosomes plus 14 Th. intermedium chromosomes, with one pair of Th. intermedium chromosomes being markedly smaller. Multicolor GISH results indicated that Zhong 4 also contained two pairs of reciprocally translocated chromosomes involving the A and D genomes. Zhong 3, Zhong 4 and Zhong 5 contained a specific gliadin band from Th. intermedium. Based on the above data, it was concluded that inter-genomic transfer of chromosomal segments and/or sequence introgression had occurred in these newly synthesized partial amphiploids despite their diploid-like meiotic behavior and disomic inheritance.

Authors+Show Affiliations

Han F
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, 960 Carling Ave, Bldg 50, Ottawa, ON, Canada, K1A 0C6. hanf@missouri.edu
Liu B
No affiliation info available
Fedak G
No affiliation info available
Liu Z
No affiliation info available

MeSH

BreedingChromosomes, PlantFluorescenceGene Transfer TechniquesGenetic VariationGenome, PlantGliadinGlutensHybridization, GeneticIn Situ HybridizationPloidiesSeedsTriticum

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15197444

Citation

Han, Fangpu, et al. "Genomic Constitution and Variation in Five Partial Amphiploids of wheat--Thinopyrum Intermedium as Revealed By GISH, Multicolor GISH and Seed Storage Protein Analysis." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 109, no. 5, 2004, pp. 1070-6.
Han F, Liu B, Fedak G, et al. Genomic constitution and variation in five partial amphiploids of wheat--Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis. Theor Appl Genet. 2004;109(5):1070-6.
Han, F., Liu, B., Fedak, G., & Liu, Z. (2004). Genomic constitution and variation in five partial amphiploids of wheat--Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 109(5), 1070-6.
Han F, et al. Genomic Constitution and Variation in Five Partial Amphiploids of wheat--Thinopyrum Intermedium as Revealed By GISH, Multicolor GISH and Seed Storage Protein Analysis. Theor Appl Genet. 2004;109(5):1070-6. PubMed PMID: 15197444.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic constitution and variation in five partial amphiploids of wheat--Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis. AU - Han,Fangpu, AU - Liu,Bao, AU - Fedak,George, AU - Liu,Zhaohui, Y1 - 2004/06/10/ PY - 2004/02/16/received PY - 2004/05/01/accepted PY - 2004/6/16/pubmed PY - 2004/12/22/medline PY - 2004/6/16/entrez SP - 1070 EP - 6 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 109 IS - 5 N2 - Genomic in situ hybridization (GISH) and multicolor GISH (mcGISH) methodology were used to establish the cytogenetic constitution of five partial amphiploid lines obtained from wheat x Thinopyrum intermedium hybridizations. Line Zhong 1, 2 n=52, contained 14 chromosomes from each of the wheat genomes plus ten Th. intermedium chromosomes, with one pair of A-genome chromosomes having a Th. intermedium chromosomal segment translocated to the short arm. Line Zhong 2, 2 n=54, had intact ABD wheat genome chromosomes plus 12 Th. intermedium chromosomes. The multicolor GISH results, using different fluorochrome labeled Th. intermedium and the various diploid wheat genomic DNAs as probes, indicated that both Zhong 1 and Zhong 2 contained one pair of Th. intermedium chromosomes with a significant homology to the wheat D genome. High-molecular-weight (HMW) glutenin and gliadin analysis revealed that Zhong 1 and Zhong 2 had identical banding patterns that contained all of the wheat bands and a specific HMW band from Th. intermedium. Zhong 1 and Zhong 2 had good HMW subunits for wheat breeding. Zhong 3 and Zhong 5, both 2 n=56, possessed no gross chromosomal aberrations or translocations that were detectable at the GISH level. Zhong 4 also had a chromosome number of 2 n=56 and contained the complete wheat ABD-genome chromosomes plus 14 Th. intermedium chromosomes, with one pair of Th. intermedium chromosomes being markedly smaller. Multicolor GISH results indicated that Zhong 4 also contained two pairs of reciprocally translocated chromosomes involving the A and D genomes. Zhong 3, Zhong 4 and Zhong 5 contained a specific gliadin band from Th. intermedium. Based on the above data, it was concluded that inter-genomic transfer of chromosomal segments and/or sequence introgression had occurred in these newly synthesized partial amphiploids despite their diploid-like meiotic behavior and disomic inheritance. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/15197444/Genomic_constitution_and_variation_in_five_partial_amphiploids_of_wheat__Thinopyrum_intermedium_as_revealed_by_GISH_multicolor_GISH_and_seed_storage_protein_analysis_ DB - PRIME DP - Unbound Medicine ER -