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alphaB-crystallin as a marker of lymph node involvement in breast carcinoma.
Cancer. 2004 Jun 15; 100(12):2543-8.C

Abstract

BACKGROUND

It was found previously that alphaB-crystallin, a small heat-shock protein, was overexpressed in a metastatic variant of the GI101A human breast carcinoma cell line. The objective of the current study was to determine whether the expression of alphaB-crystallin in primary breast carcinomas was associated with lymph node metastasis and survival.

METHODS

Expression of alphaB-crystallin was measured in human breast carcinoma cell lines by immunoblotting. Expression in human breast carcinomas was evaluated by immunohistochemical staining of tissue microarrays that contained samples from 317 patients with lymph node-negative breast carcinoma and 291 patients with lymph node-positive breast carcinoma.

RESULTS

It was found that alphaB-crystallin was expressed constitutively in certain breast carcinoma cell lines, including those that were capable of metastasizing in immunodeficient mice. Expression of alphaB-crystallin in human tissue samples was associated strongly with lymph node involvement (P < 0.0001; chi-square test) and, to a lesser degree, with high nuclear grade (P = 0.05). Increased intensity of expression was correlated with shorter survival (P = 0.0091; log-rank test). Multivariate analysis indicated that alphaB-crystallin expression was not independent of lymph node status as a predictor of survival.

CONCLUSIONS

The data obtained in the current study revealed a strong association between high expression levels of alphaB-crystallin in primary breast carcinoma specimens and lymph node involvement. Further studies will be needed to prospectively elucidate the role of this novel tumor marker as a clinical prognostic marker in local and locally advanced breast carcinoma as well as its potential status as a new target for therapy in patients with breast carcinoma.

Authors+Show Affiliations

Department of Cancer Biology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15197794

Citation

Chelouche-Lev, Dina, et al. "AlphaB-crystallin as a Marker of Lymph Node Involvement in Breast Carcinoma." Cancer, vol. 100, no. 12, 2004, pp. 2543-8.
Chelouche-Lev D, Kluger HM, Berger AJ, et al. AlphaB-crystallin as a marker of lymph node involvement in breast carcinoma. Cancer. 2004;100(12):2543-8.
Chelouche-Lev, D., Kluger, H. M., Berger, A. J., Rimm, D. L., & Price, J. E. (2004). AlphaB-crystallin as a marker of lymph node involvement in breast carcinoma. Cancer, 100(12), 2543-8.
Chelouche-Lev D, et al. AlphaB-crystallin as a Marker of Lymph Node Involvement in Breast Carcinoma. Cancer. 2004 Jun 15;100(12):2543-8. PubMed PMID: 15197794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - alphaB-crystallin as a marker of lymph node involvement in breast carcinoma. AU - Chelouche-Lev,Dina, AU - Kluger,Harriet M, AU - Berger,Aaron J, AU - Rimm,David L, AU - Price,Janet E, PY - 2004/6/16/pubmed PY - 2004/6/24/medline PY - 2004/6/16/entrez SP - 2543 EP - 8 JF - Cancer JO - Cancer VL - 100 IS - 12 N2 - BACKGROUND: It was found previously that alphaB-crystallin, a small heat-shock protein, was overexpressed in a metastatic variant of the GI101A human breast carcinoma cell line. The objective of the current study was to determine whether the expression of alphaB-crystallin in primary breast carcinomas was associated with lymph node metastasis and survival. METHODS: Expression of alphaB-crystallin was measured in human breast carcinoma cell lines by immunoblotting. Expression in human breast carcinomas was evaluated by immunohistochemical staining of tissue microarrays that contained samples from 317 patients with lymph node-negative breast carcinoma and 291 patients with lymph node-positive breast carcinoma. RESULTS: It was found that alphaB-crystallin was expressed constitutively in certain breast carcinoma cell lines, including those that were capable of metastasizing in immunodeficient mice. Expression of alphaB-crystallin in human tissue samples was associated strongly with lymph node involvement (P < 0.0001; chi-square test) and, to a lesser degree, with high nuclear grade (P = 0.05). Increased intensity of expression was correlated with shorter survival (P = 0.0091; log-rank test). Multivariate analysis indicated that alphaB-crystallin expression was not independent of lymph node status as a predictor of survival. CONCLUSIONS: The data obtained in the current study revealed a strong association between high expression levels of alphaB-crystallin in primary breast carcinoma specimens and lymph node involvement. Further studies will be needed to prospectively elucidate the role of this novel tumor marker as a clinical prognostic marker in local and locally advanced breast carcinoma as well as its potential status as a new target for therapy in patients with breast carcinoma. SN - 0008-543X UR - https://www.unboundmedicine.com/medline/citation/15197794/alphaB_crystallin_as_a_marker_of_lymph_node_involvement_in_breast_carcinoma_ L2 - https://doi.org/10.1002/cncr.20304 DB - PRIME DP - Unbound Medicine ER -