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Pronlonged hypothermic machine perfusion preserves hepatocellular function but potentiates endothelial cell dysfunction in rat livers.
Transplantation. 2004 Jun 15; 77(11):1676-82.T

Abstract

BACKGROUND

Although hypothermic machine perfusion (HMP) preservation has been shown to improve organ function and to expand the organ donor pool, problems still exist with the current HMP technology for liver preservation. The present study was conducted to investigate endothelial and hepatocellular functions following extended HMP (> r =24 hr) in rat liver model.

METHODS

Following 24-hour hypothermic HMP with University of Wisconsin (UW) solution or 24-hour simple cold storage (SCS), livers were reperfused with Krebs-Henseleit buffer solution at 37 degree C for 30 minutes. Hepatocyte damage and function were assessed by measuring lactate dehydrogenase (LDH) activity, bile production, and indocyanine green (ICG) extraction. Sinusoidal endothelial cell (SEC) function and permeability were determined by hyaluronic acid (HA) uptake and multiple indicator dilution (MID) method, respectively.

RESULTS

After 24-hour hypothermic preservation, HMP livers showed lower released LDH levels, higher bile flow rate, and greater hepatic ICG uptake compared with SCS livers. However, LDH levels became significantly higher in HMP than in SCS after 30 minutes of warm perfusion. The increased enzyme levels were accompanied by a significant increase in endothelial permeability to albumin and a decrease in hyaluronic acid uptake in HMP compared to SCS. Liver wet/dry weight ratio confirmed a greater edema in HMP livers than SCS livers.

CONCLUSION

These results suggest that 24-hour hypothermic HMP may help preservation of hepatocyte function, but endothelial cell dysfunction during the cold preservation may play a key role in hepatocyte dysfunction and parenchymal cell death upon reperfusion.

Authors+Show Affiliations

Department of Biology, University of North Carolina, Charlotte, NC 28223, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15201666

Citation

Xu, Hongzhi, et al. "Pronlonged Hypothermic Machine Perfusion Preserves Hepatocellular Function but Potentiates Endothelial Cell Dysfunction in Rat Livers." Transplantation, vol. 77, no. 11, 2004, pp. 1676-82.
Xu H, Lee CY, Clemens MG, et al. Pronlonged hypothermic machine perfusion preserves hepatocellular function but potentiates endothelial cell dysfunction in rat livers. Transplantation. 2004;77(11):1676-82.
Xu, H., Lee, C. Y., Clemens, M. G., & Zhang, J. X. (2004). Pronlonged hypothermic machine perfusion preserves hepatocellular function but potentiates endothelial cell dysfunction in rat livers. Transplantation, 77(11), 1676-82.
Xu H, et al. Pronlonged Hypothermic Machine Perfusion Preserves Hepatocellular Function but Potentiates Endothelial Cell Dysfunction in Rat Livers. Transplantation. 2004 Jun 15;77(11):1676-82. PubMed PMID: 15201666.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pronlonged hypothermic machine perfusion preserves hepatocellular function but potentiates endothelial cell dysfunction in rat livers. AU - Xu,Hongzhi, AU - Lee,Charles Y, AU - Clemens,Mark G, AU - Zhang,Jian X, PY - 2004/6/18/pubmed PY - 2004/7/14/medline PY - 2004/6/18/entrez SP - 1676 EP - 82 JF - Transplantation JO - Transplantation VL - 77 IS - 11 N2 - BACKGROUND: Although hypothermic machine perfusion (HMP) preservation has been shown to improve organ function and to expand the organ donor pool, problems still exist with the current HMP technology for liver preservation. The present study was conducted to investigate endothelial and hepatocellular functions following extended HMP (> r =24 hr) in rat liver model. METHODS: Following 24-hour hypothermic HMP with University of Wisconsin (UW) solution or 24-hour simple cold storage (SCS), livers were reperfused with Krebs-Henseleit buffer solution at 37 degree C for 30 minutes. Hepatocyte damage and function were assessed by measuring lactate dehydrogenase (LDH) activity, bile production, and indocyanine green (ICG) extraction. Sinusoidal endothelial cell (SEC) function and permeability were determined by hyaluronic acid (HA) uptake and multiple indicator dilution (MID) method, respectively. RESULTS: After 24-hour hypothermic preservation, HMP livers showed lower released LDH levels, higher bile flow rate, and greater hepatic ICG uptake compared with SCS livers. However, LDH levels became significantly higher in HMP than in SCS after 30 minutes of warm perfusion. The increased enzyme levels were accompanied by a significant increase in endothelial permeability to albumin and a decrease in hyaluronic acid uptake in HMP compared to SCS. Liver wet/dry weight ratio confirmed a greater edema in HMP livers than SCS livers. CONCLUSION: These results suggest that 24-hour hypothermic HMP may help preservation of hepatocyte function, but endothelial cell dysfunction during the cold preservation may play a key role in hepatocyte dysfunction and parenchymal cell death upon reperfusion. SN - 0041-1337 UR - https://www.unboundmedicine.com/medline/citation/15201666/Pronlonged_hypothermic_machine_perfusion_preserves_hepatocellular_function_but_potentiates_endothelial_cell_dysfunction_in_rat_livers_ L2 - https://doi.org/10.1097/01.tp.0000129644.23075.71 DB - PRIME DP - Unbound Medicine ER -